FastQCFastQC Report
Tue 8 Oct 2019
HVNVGAFXY_n01_undetermined.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHVNVGAFXY_n01_undetermined.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences27182409
Sequences flagged as poor quality0
Sequence length151
%GC78

[WARN]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TAAGTAGAGTCTTGTGGAAAGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG11478494.222764067746902No Hit
GGGGGGCGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG9979583.6713375918962883No Hit
GGGGGGCGGGGGGGGGCGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG5700172.097006928267469No Hit
CGATCAAGATCGTCTTGTGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG5562572.0463859549755137No Hit
ATACACGATCTCTTGTGGAAGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG5119721.883468091441049No Hit
CGATCAAGATCGTCTTCTGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG5059481.8613067002266064No Hit
CGATCAAGATCGTCTACTGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG4784541.7601604037375789No Hit
GATCGCGCGGTTCTTGTGGAGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG4219661.552349536054733No Hit
TCGATCCTTACCATCTAGTGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG3781421.391127622279541No Hit
GATCGCGCGGTTCTTGAGGAGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG3768611.386415015681649No Hit
TAAGTAGAGTCTTGTGGAAAGGACGAAACACCGAACCCCTACCAACTGGT3213511.1822020631063275No Hit
ATACACGATCTCTTGTCGAAGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG3046601.1207983810412094No Hit
CGATCAAGATCGTCTAGTGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG2625850.9660107755718045No Hit
ATACACGATCTCTTGACGAAGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG2441600.8982279679479476No Hit
TCGATCCTTACCATCAAGTGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG2408910.886201807941305No Hit
ATACAGGATCTCTTGTGGAAGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG2404830.8847008372216016No Hit
GATCGGGCGGTTCTTGTGGAGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG2321160.8539199009182741No Hit
CGATCAAGATCGTCTACTGGAAAGGACGAAACACCGAACCCCTACCAACT2058340.7572323703907184No Hit
TAAGTTGAGTCTTGTGGAAAGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG2038190.7498194880372818No Hit
ATACACGATCTCTTGAGGAAGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG1982610.7293724408311273No Hit
CGATCAAGATCGTCTTCTGGAAAGGACGAAACACCGAACCCCTACCAACT1961790.7217130755408765No Hit
TCGATCCTTACCATCTTGTGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG1873530.6892435471778826No Hit
CGATCTAGATCGTCTAGTGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG1771100.6515610886437622No Hit
GATCGCGCGGTTCTTGAGGAAAGGACGAAACACCGAACCCCTACCAACTG1754340.6453953363736084No Hit
TCGATCCATACCATCAAGTGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG1739480.6399285655660615No Hit
CGATCTAGATCGTCTTGTGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG1713760.6304665638722454No Hit
TAAGTAGAGTCTTGTGCAAAGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG1644860.605119288728236No Hit
TAAGTAGAGTCTTGTCGAAAGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG1593080.5860702044472953No Hit
ATACACGATCTCTTGTCGAAAGGACGAAACACCGAACCCCTACCAACTGG1569220.5772924688168735No Hit
ATACACGATCTCTTGACGAAAGGACGAAACACCGAACCCCTACCAACTGG1412060.5194756653098701No Hit
TAAGTAGAGTCTTGTCGAAAGGACGAAACACCGAACCCCTACCAACTGGT1246380.45852448177054506No Hit
GATCGGGCGGTTCTTGAGGAGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG1195530.43981753052130146No Hit
TCGATCCTTACCATCTAGTGGAAAGGACGAAACACCGAACCCCTACCAAC1195160.4396814130785832No Hit
TCGATCCATACCATCTAGTGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG1176420.4327872485473969No Hit
CGATCATGATCGTCTTGTGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG1077160.39627098540088923No Hit
ATACACGATCTCTTGTGGAAAGGACGAAACACCGAACCCCTACCAACTGG1069280.39337205175597206No Hit
GATCGCGCGGTTCTTCAGGAGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG1013350.3727962448067057No Hit
ATACAGGATCTCTTGTCGAAGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG1002150.3686759330271279No Hit
TAAGTACAGTCTTGTGGAAAGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG988150.3635255433026558No Hit
CGATCAAGATCGTCTTGTGGAAAGGACGAAACACCGAACCCCTACCAACT977310.359537670115993No Hit
GATCGCGCGGTTCTTCAGGAAAGGACGAAACACCGAACCCCTACCAACTG950320.3496084544971713No Hit
ATACAGGATCTCTTGAGGAAGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG912790.3358017311857827No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG898540.33055937021623066No Hit
GATCGCGCGGTTCTTGTGGAAAGGACGAAACACCGAACCCCTACCAACTG861380.31688876434756025No Hit
GATAGCGCGGTTCTTGAGGAGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG758030.278867851631546No Hit
ATACACGATCTCTTGAGGAGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG750000.27591373523958085No Hit
TCGATCCTTACCATCAAGTGGAAAGGACGAAACACCGAACCCCTACCAAC747160.2748689418954737No Hit
TAAGTAGAGTCTTGTGGAAAGGACGAAACACCGAACCCGTACCAACTGGT725450.2668821589727386No Hit
TCGATCCATACCATCTTGTGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG664600.24449635792030058No Hit
TCGATGCTTACCATCAAGTGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG636000.23397484748316458No Hit
GGGGGGCGGGGGGGGCCGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG618160.22741177943426577No Hit
TCGATCGTTACCATCAAGTGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG590560.2172581539774492No Hit
GATCGCGCGGTTCTTGAGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG577110.21231010099215267No Hit
TCGATGCATACCATCTTGTGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG567830.20889612837478827No Hit
GATCGCTCGGTTCTTGAGGAGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG566950.20857238959210717No Hit
TAAGTAGAGTCTTGTGGAAAGGACGAAACACCGAACCGCTACCAACTGGT556820.2048457147414712No Hit
ATACAGGATCTCTTGACGAAGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG546750.20114111298965442No Hit
TCGATCGTTACCATCTTGTGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG528880.19456700839134605No Hit
CGATCAAGATCGTCTTGTGGAAAGGACGAAACACCGATCCCCTACCAACT528830.19454861414233005No Hit
TAAGTAGAGTCTTGTGCAAAGGACGAAACACCGAACCCCTACCAACTGGT526520.19369879983779217No Hit
CGATCATGATCGTCTAGTGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG498380.18334651649160308No Hit
TAAGTAGAGTCTTGTGGAAAGGACGAAACACCGAACCGGTACCATCTGGT492940.1813452221986653No Hit
TCGATCGTTACCATCTAGTGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG484870.17837639040748743No Hit
ATAAACGATCTCTTGTGGAAGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG450850.16586094337702004No Hit
GATAGCGCGGTTCTTGTGGAGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG450140.16559974504099323No Hit
ATAAACGATCTCTTGACGAAGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG445010.1637124950919545No Hit
TCGATCCATACCATCTAGTGGAAAGGACGAAACACCGAACCCCTACCAAC441670.16248375925768757No Hit
TAAGTAGAGTCTTGTGGAAAGGACGAAACACCGAACCCCTACCATCTGGT441120.16228142251851188No Hit
CGATCTTGATCGTCTAGTGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG433050.159312590727334No Hit
TAAGTAGAGTCTTGTGGAAAGGACGAAACACCGAACCCGTACCATCTGGT428530.1576497506162901No Hit
CGATCTAGATCGTCTACTGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG420090.15454480138239404No Hit
CGATCATGATCGTCTTCTGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG417520.15359933698297307No Hit
TCGATCCATACCATCTAGTGGAAAGGACGATTCACCGATCCCCTACCAAC416750.1533160655481271No Hit
CGATCTAGATCGTCTTCTGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG411640.15143617329869474No Hit
TAAGTAGAGTCTTGTGGAAAGGACGAAACACCGAACCGCTACCATCTGGT398720.14668309935296758No Hit
GGGGGGGGGGGGGGGGCGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG390970.1438319907554919No Hit
TCGATGCTTACCATCTAGTGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG384240.14135612483794205No Hit
CGATCAAGATCGTCTAGTGGAAAGGACGAAACACCGAACCCCTACCAACT384210.14134508828853248No Hit
TCGATGCTTACCATCTTGTGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG382340.14065714337533514No Hit
TAAGTAGAGTCTTGTGGAAAGGACGAAACACCGAACCCCTACCAACAGGT370560.13632345830717213No Hit
ATACACCATCTCTTGTGGAAGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG361180.13287269719177575No Hit
GGGGGGCGGGGGGGGCGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG355710.1308603663494284No Hit
GATAGCGCGGTTCTTCAGGAGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG350060.12878181621062357No Hit
ATACACGATCTCTTGAGGAAAGGACGAAACACCGAACCCCTACCAACTGG349590.12860891026987342No Hit
ATACAGCATCTCTTGTGGAAGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG342690.1260705039056693No Hit
TAAGTTGAGTCTTGTGGACAGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG339230.12479762187376402No Hit
CGATCAAGATCGACTACTGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG334850.12318628565996487No Hit
GATCGCGCGGTTCTTGAGGAAAGGACGAAACTCCGAACCCCTACCAACTG333430.12266388898791127No Hit
TAAGTAGAGTCTTGTGGAAGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG323130.11887467369062102No Hit
TAAGTAGAGTCTTGTGGAGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG311870.11473228881222412No Hit
TCGATCCTTACCATCATGTGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG310350.11417310364213856No Hit
CGATCTTGATCGTCTTGTGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG307520.11313198914783454No Hit
TAAGTTCAGTCTTGTGGAAAGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG306340.1126978848710576No Hit
CGATCAAGATCGTCTACTGGAAAGGACGAAACACCGATCCCCTACCAACT306240.11266109637302565No Hit
GATCGCGCGGTTCTTCAGGAAAGGACGAAACTCCGAACCCCTACCAACTG305080.11223434979585512No Hit
TCGATCCATACCATCAAGTGGAAAGGACGAAACACCGAACCCCTACCAAC304240.11192532641238677No Hit
ATAAACGATCTCTTGAGGAAGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG303760.11174874162183344No Hit
TAAGTAGAGTCTTGTGGAAAGGACGAAACACCGAACCGGTACCAACTGGT302810.11139925089052997No Hit
TAAGTAGAGTCTTGTCCAAAGGACGAAACACCGAACCCCTACCAACTGGT296300.1090043196686504No Hit
CGATCAAGATCGTCTTCTGGAAAGGACGAAACACCGATCCCCTACCAACT295650.10876519443144277No Hit
CGATCAAGATCGTCTAGTGGAAAGGACGAAACACCGATCCCCTACCAACT292560.10762842984225571No Hit
TCGATGCATACCATCTAGTGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG291070.10708028122157973No Hit
ATAAACGATCTCTTGACGAAAGGACGAAACACCGAACCCCTACCAACTGG282050.10376195869909839No Hit
ATAAACGATCTCTTGTCGAAGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG279920.10297836369101795No Hit
TAAGTAGAGTCTAGTGGAAAGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG278380.10241182082132604No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACACGA3138100.0144.264532
TAGAGTC3951800.0144.213785
TAAGTAG3903350.0144.208391
GTAGAGT3946850.0144.122044
GATCAAG4219050.0144.090072
GATCGCG2446650.0144.076951
AAGTAGA3925350.0144.047122
TCCTTAC1835150.0144.044625
GCGGTTC3775950.0144.012397
AGTAGAG3947850.0143.986773
AGTCTTG4922300.0143.98638
ATCAAGA4259950.0143.982793
CACGATC3173250.0143.954214
GATCGTC5823750.0143.948878
ACACGAT3177000.0143.929763
CCTTACC1837500.0143.915426
TCTCTTG5359000.0143.906989
TTACCAT2834050.0143.891048
ATCGTCT5830300.0143.889929
ATCGGGC1002050.0143.863822
Produced by FastQC (version 0.11.5)