FastQCFastQC Report
Tue 8 Oct 2019
HVNVGAFXY_n01_PCR2_R6_rc.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHVNVGAFXY_n01_PCR2_R6_rc.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences27614302
Sequences flagged as poor quality0
Sequence length151
%GC49

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TAAGTAGAGTCTTGTGGAAAGGACGAAACACCGAACCCCTACCAACTGGT12272184.444139127615827No Hit
CGATCAAGATCGTCTTCTGGAAAGGACGAAACACCGAACCCCTACCAACT8736293.1636830798765074No Hit
ATACACGATCTCTTGTCGAAAGGACGAAACACCGAACCCCTACCAACTGG7168112.5957961928568754No Hit
GATCGCGCGGTTCTTGAGGAAAGGACGAAACACCGAACCCCTACCAACTG6578962.382446603213074No Hit
ATACACGATCTCTTGTGGAAAGGACGAAACACCGAACCCCTACCAACTGG6084592.203419807605494No Hit
CGATCAAGATCGTCTTGTGGAAAGGACGAAACACCGAACCCCTACCAACT5956222.1569330269510343No Hit
CGATCAAGATCGTCTACTGGAAAGGACGAAACACCGAACCCCTACCAACT5327651.9293082258606427No Hit
TCGATCCTTACCATCTAGTGGAAAGGACGAAACACCGAACCCCTACCAAC5273311.9096300170831766No Hit
GATCGCGCGGTTCTTGTGGAAAGGACGAAACACCGAACCCCTACCAACTG4859741.7598634214980338No Hit
ATACACGATCTCTTGACGAAAGGACGAAACACCGAACCCCTACCAACTGG3353671.2144685025897086No Hit
TCGATCCATACCATCTAGTGGAAAGGACGAAACACCGAACCCCTACCAAC2994661.0844597846434794No Hit
TAAGTAGAGTCTTGTCGAAAGGACGAAACACCGAACCCCTACCAACTGGT2687140.9730972015877859No Hit
TAAGTAGAGTCTTGTGCAAAGGACGAAACACCGAACCCCTACCAACTGGT2615160.9470309986470055No Hit
GATCGCGCGGTTCTTCAGGAAAGGACGAAACACCGAACCCCTACCAACTG2442010.8843279833761505No Hit
TAAGTAGAGTCTTGTGGAAAGGACGAAACACCGAACCCCTACCATCTGGT2025530.7335075860327739No Hit
TCGATCCTTACCATCAAGTGGAAAGGACGAAACACCGAACCCCTACCAAC1701420.6161372465615824No Hit
TCGATCCTTACCATCTTGTGGAAAGGACGAAACACCGAACCCCTACCAAC1573340.569755483951758No Hit
TAAGTAGAGTCTTGTGGAAAGGACGAAACACCGAACCCGTACCATCTGGT1414870.5123685545265638No Hit
CGATCAAGATCGTCTAGTGGAAAGGACGAAACACCGAACCCCTACCAACT1184390.4289045582249372No Hit
TAAGTAGAGTCTTGTGGAAAGGACGAAACACCGAACCGCTACCATCTGGT944260.3419459959552843No Hit
TAAGTAGAGTCTTGTGGAAAGGACGAAACACCGAACCGCTACCAACTGGT916340.3318352931752539No Hit
TAAGTAGAGTCTTGTGGAAAGGACGAAACACCGAACCCGTACCAACTGGT884050.32014207710193077No Hit
ATACACGATCTCTTGAGGAAAGGACGAAACACCGAACCCCTACCAACTGG799180.2894080031427193No Hit
TCGATCCATACCATCAAGTGGAAAGGACGAAACACCGAACCCCTACCAAC773650.2801627939029565No Hit
TCGATCCATACCATCTTGTGGAAAGGACGAAACACCGAACCCCTACCAAC755940.27374945055645444No Hit
TAAGTAGAGTCTTGTGGAAAGGACGAAACACCGAACCGGTACCATCTGGT740950.2683211040423908No Hit
GATAGCGCGGTTCTTGAGGAAAGGACGAAACACCGAACCCCTACCAACTG740220.2680567482748613No Hit
TCGATCCTTACCATCTAGTGGAAAGGACGATTCACCGATCCCCTTCCAAC715370.25905778824320813No Hit
ATAAACGATCTCTTGTCGAAAGGACGAAACACCGAACCCCTACCAACTGG696680.2522895563320775No Hit
GATAGCGCGGTTCTTGTGGAAAGGACGAAACACCGAACCCCTACCAACTG696350.25217005303990664No Hit
CGATCAAGATCGTCTACTGGAAAGGACGAAACACCGATCCCCTACCAACT674690.24432629149923835No Hit
CGATCAAGATCGTCTTGTGGAAAGGACGAAACACCGATCCCCTACCAACT669750.2425373634285596No Hit
GATCGCGCGGTTCTTGAGGAAAGGACGAATCTCCGAACGCCTACCTACTG653560.2366744594884202No Hit
ATACACGATCTCTTGTGGAAAGGACGAAACTCCGAACCGCTACCTTCTGG638430.23119541460798104No Hit
GATCGGGCGGTTCTTGTGGAAAGGACGAAACTCCGAACGCCTACCTACTG627520.22724456334257517No Hit
ATAAACGATCTCTTGTGGAAAGGACGAAACACCGAACCCCTACCAACTGG624980.22632475012404804No Hit
ATACACGATCTCTTGTCGAAAGGACGAAACTCCGAACCGCTACCTTCTGG622740.22551357626203988No Hit
TAAGTTGAGTCTTGTGGAAAGGACGAAACACCGAACCCCTACCAACTGGT618640.22402883838961418No Hit
CGATCAAGATCGTCTTCTGGAAAGGACGAAACACCGATCCCCTACCAACT600630.21750685568659314No Hit
GATCGCGCGGTTCTTGAGGAAAGGACGAAACTCCGAACCCCTACCTACTG583860.21143391565718372No Hit
GATCGGGCGGTTCTTGAGGAAAGGACGAATCTCCGAACGCCTACCTACTG576550.2087867366700053No Hit
CGATCAAGATCGTCTTGTGGAAAGGACGATTCACCGATCCCCTACCAACT571440.20693624629729915No Hit
GATCGCGCGGTTCTTGAGGAAAGGACGAAACTCCGAACGCCTACCTACTG555630.20121095220875038No Hit
CGATCAAGATCGTCTAGTGGAAAGGACGAAACACCGATCCCCTACCAACT549060.19883175030098532No Hit
CGATCAAGATCGTCTAGTGGAAAGGACGATTCACCGATCCCCTACCAACT538670.19506920725354562No Hit
ATACACGATCTCTTGTGGAAAGGACGAAACACCGAACCGCTACCTTCTGG519630.18817422942647616No Hit
GATCGGGCGGTTCTTGAGGAAAGGACGAAACTCCGAACGCCTACCTACTG510550.1848860782358359No Hit
CGATCATGATCGTCTTGTGGAAAGGACGAAACACCGAACCCCTACCAACT495670.1794975661524959No Hit
GATCGCGCGGTTCTTGTGGAAAGGACGAAACTCCGAACCCCTACCTACTG493980.1788855644441058No Hit
TAAGTACAGTCTTGTGGAAAGGACGAAACACCGAACCCCTACCAACTGGT483220.1749890328569594No Hit
TCGATCCTTACCATCTAGTGGAAAGGACGAAACACCGATCCCCTACCAAC479820.1737577868164113No Hit
CGATCAAGATCGTCTTCTGGAAAGGACGAATCACCGAACCCCTACCAACT477790.1730226605039664No Hit
TAAGTAGAGTCTTGTGGAAAGGACGAAACACCGAACCGGTACCAACTGGT474370.17178417183965033No Hit
CGATCAAGATCGTCTTGTGGAAAGGACGAATCACCGAACCCCTACCAACT467370.1692492535208748No Hit
GATCGCGCGGTTCTTGAGGAAAGGACGAATCTCCGAACCCCTACCTACTG458090.16588867609255523No Hit
TAAGTAGAGTCTTGTGGAAAGGACGAAACTCCGAACCGGTACCATCTGGT455020.16477693334417795No Hit
GATCGCGCGGTTCTTGAGGAAAGGACGAAACACCGAACCCCTACCTACTG451770.1636000069818893No Hit
GATCGCGCGGTTCTTGTGGAAAGGACGAAACTCCGAACGCCTACCTACTG445510.16133306574252718No Hit
TAAGTTGAGTCTTGTGGAAAGGACGAAACACCGAACCCGTACCATCTGGT444990.161144757524561No Hit
ATACACGATCTCTTGTGGAAAGGACGAAACACCGAACCGCTACCATCTGG444220.1608659165094957No Hit
ATACACGATCTCTTGTCGAAAGGACGAAACACCGAACCCCTACCTTCTGG442560.1602647787367575No Hit
TAAGTAGAGTCTTGTCGAAAGGACGAAACACCGAACCCCTACCATCTGGT442060.16008371314255923No Hit
GATAGCGCGGTTCTTGAGGAAAGGACGAAACTCCGAACCCCTACCTACTG437390.15839256049274755No Hit
TCGATCCTTACCATCTAGTGGAAAGGACGATTCACCGATCCCCTACCAAC421790.15274331395376206No Hit
TAAGTTGAGTCTTGTGGAAAGGACGAAACACCGAACCCGTACCAACTGGT404580.14651103620145822No Hit
TAAGTTGAGTCTTGTGGAAAGGACGAAACACCGAACCGGTACCATCTGGT384990.13941688622077067No Hit
TAAGTAGAGTCTTGTGGAAAGGACGAAACTCCGAACCGGTACCAACTGGT377870.13683851215938755No Hit
GATCGCGCGGTTCTTGAGGAAAGGACGAAACTCCGAACGCCTACGTACTG368330.1333837806220849No Hit
GATCGCGCGGTTCTTGAGGAAAGGACGAAACTCCGAACCCCTACCAACTG361260.13082351312012158No Hit
ATACACGATCTCTTGTCGAAAGGACGAAACACCGAACCCCTACCATCTGG359790.13029118027317874No Hit
ATACACCATCTCTTGTCGAAAGGACGAAACACCGAACCCCTACCAACTGG358340.1297660900500038No Hit
CGATCTAGATCGTCTTGTGGAAAGGACGAAACACCGAACCCCTACCAACT358070.12966831462913672No Hit
TAAGTAGAGTCTTGTGGAAAGGACGAAACACCGAACCCCAACCAACTGGT354420.1283465357914895No Hit
TCGATCCTTACCATCTTGTGGAAAGGACGAAACACCGATCCCCTACCAAC348230.12610494373531514No Hit
TAAGTAGAGTCTTGTGGAAAGGACGAAACTCCGAACCCGTACCAACTGGT346280.12539878791794193No Hit
GATCGCGCGGTTCTTGTGGAAAGGACGAAACTCCGAACGCCTACGTACTG339980.12311736143104396No Hit
ATACACGATCTCTTGTGGAAAGGACGAAACACCGAACCCCTACCATCTGG339960.12311011880727603No Hit
ATACAGGATCTCTTGTGGAAAGGACGAAACTCCGAACCGCTACCATCTGG339660.12300147945075708No Hit
TCGATCCTTACCATCAAGTGGAAAGGACGATTCACCGATCCCCTTCCAAC336790.1219621629400591No Hit
ATACACGATCTCTTGTGGAAAGGACGAAACACCGAACCGCTACCAACTGG336710.12193319244498738No Hit
GATCGCGCGGTTCTTGTGGAAAGGACGAATCTCCGAACGCCTACCTACTG336700.12192957113310342No Hit
GATCGGGCGGTTCTTGAGGAAAGGACGAAACTCCGAACCCCTACCTACTG335530.1215058776426795No Hit
CGATCAAGATCGTCTAGTGGAAAGGACGAATCACCGATCCCCTACCAACT334460.12111839727109523No Hit
TCGATCCTTACCATCTTGTGGAAAGGACGATTCACCGATCCCCTTCCAAC331800.12015512830996053No Hit
CGATCAAGATCGTCTTCTGGAAAGGACGATTCACCGATCCCCTACCAACT323200.1170408000897506No Hit
TCGATCCTTACCATCTAGTGGAAAGGACGAATCACCGATCCCCTACCAAC319700.11577334093036283No Hit
ATACAGGATCTCTTGAGGAAAGGACGAAACTCCGAACCGCTACCATCTGG317750.11506718511298963No Hit
ATACACGATCTCTTGTCGAAAGGACGAAACACCGAACCGCTACCTTCTGG315970.11442259159764386No Hit
TAAGTAGAGTCTTGTGGAAAGGACGAAACTCCGAACCCCTACCAACTGGT315920.11440448503822404No Hit
GATCGGGCGGTTCTTGAGGAAAGGACGAAACTCCGAACGCCTACGTACTG311860.11293423241333421No Hit
GATCGGGCGGTTCTTGTGGAAAGGACGAAACTCCGAACCCCTACCTACTG309630.11212667986321002No Hit
ATACACCATCTCTTGACGAAAGGACGAAACACCGAACCCCTACCAACTGG309270.11199631263538727No Hit
TCGATCGTTACCATCTTGTGGAAAGGACGAAACACCGATCCCCTACCAAC308560.11173919949162575No Hit
GATCGCGCGGTTCTTGTGGAAAGGACGAAACTCCGAACCCCTACCAACTG306840.11111633384758376No Hit
CGATCAAGATCGTCTTGTGGAAAGGACGAAACACCGAACCCCTACGAACT304380.11022549112412836No Hit
CGATCATGATCGTCTTCTGGAAAGGACGAAACACCGAACCCCTACCAACT304080.11011685176760941No Hit
ATACACGATCTCTTGACGAAAGGACGAAACACCGAACCCCTACCTTCTGG298910.10824463352359948No Hit
TAAGTAGAGTCTTGTGGAAAGGACGAAACTCCGAACCCGTACCATCTGGT298530.10810702367200879No Hit
GATCGCGCGGTTCTTGTGGAAAGGACGAATCTCCGAACCCCTACCTACTG295620.10705322191377498No Hit
CGATCAAGATCGTCTACTGGAAAGGACGAATCACCGAACCCCTACCAACT293790.10639052183900938No Hit
TAAGTAGAGTCTTGTGGAAAGGACGAAACTCCGAACCGCTACCATCTGGT292980.1060971955764082No Hit
ATACAGGATCTCTTGTGGAAAGGACGAAACACCGAACCGCTACCTTCTGG287840.10423584126805015No Hit
CGATCAAGATCGTCTTGTGGAAAGGACGAATCACCGATCCCCTACCAACT287310.10404391173820002No Hit
CGATCAAGATCGTCTTGTGGAAAGGACGATTCACCGAACCCCTACCAACT287290.10403666911443209No Hit
TAAGTAGAGTCTTGTGGAAAGGACGAAACACCGAACCCCTACCAACAGGT280560.10159952621652359No Hit
ATACACGATCTCTTGTCGAAAGGACGAAACACCGAACCGCTACCAACTGG279560.1012373950281271No Hit
GATCGGGCGGTTCTTGTGGAAAGGACGAATCTCCGAACGCCTACCTACTG277880.10062901463162095No Hit
ATAAACGATCTCTTGACGAAAGGACGAAACACCGAACCCCTACCAACTGG276950.10029223262641221No Hit
ATACACGATCTCTTGTGGAAAGGACGAAACACCGAACCCCTACCTTCTGG276280.10004960473018656No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCGCG2900000.0144.630251
TACACGA3752400.0144.61012
TAGAGTC4344900.0144.390265
GATCAAG4598500.0144.338092
GATCGTC6447600.0144.285138
TAAGTAG4324100.0144.266281
GTAGAGT4348300.0144.094164
AAGTAGA4344450.0144.093492
CACGATC3744300.0144.08354
ATCAAGA4619400.0144.073873
ATCGTCT6443200.0144.037529
GCGGTTC4501150.0144.032557
TCCTTAC2318550.0144.015885
CAAGATC4624500.0143.84455
CCTTACC2321250.0143.829656
AGTCTTG5594050.0143.820248
AGTAGAG4351900.0143.813543
ATCGGGC1005600.0143.799382
TCTCTTG6046450.0143.774149
TCGCGCG2934700.0143.703523
Produced by FastQC (version 0.11.5)