FastQCFastQC Report
Tue 8 Oct 2019
HVNVGAFXY_n01_PCR2_R5_rc.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHVNVGAFXY_n01_PCR2_R5_rc.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences25143561
Sequences flagged as poor quality0
Sequence length151
%GC49

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TAAGTAGAGTCTTGTGGAAAGGACGAAACACCGAACCCCTACCAACTGGT9219013.6665490620043837No Hit
CGATCAAGATCGTCTTCTGGAAAGGACGAAACACCGAACCCCTACCAACT7420282.951165111417591No Hit
ATACACGATCTCTTGTCGAAAGGACGAAACACCGAACCCCTACCAACTGG6257872.488855894358003No Hit
GATCGCGCGGTTCTTGAGGAAAGGACGAAACACCGAACCCCTACCAACTG5459812.1714545525194304No Hit
ATACACGATCTCTTGTGGAAAGGACGAAACACCGAACCCCTACCAACTGG5251862.088749481427869No Hit
CGATCAAGATCGTCTACTGGAAAGGACGAAACACCGAACCCCTACCAACT5105592.0305755417858276No Hit
TCGATCCTTACCATCTAGTGGAAAGGACGAAACACCGAACCCCTACCAAC5069792.0163373040119494No Hit
CGATCAAGATCGTCTTGTGGAAAGGACGAAACACCGAACCCCTACCAACT4827171.9198434143835077No Hit
GATCGCGCGGTTCTTGTGGAAAGGACGAAACACCGAACCCCTACCAACTG3803351.5126536770189394No Hit
ATACACGATCTCTTGACGAAAGGACGAAACACCGAACCCCTACCAACTGG3343401.3297241389157248No Hit
TCGATCCATACCATCTAGTGGAAAGGACGAAACACCGAACCCCTACCAAC2704501.075623297750068No Hit
TAAGTAGAGTCTTGTCGAAAGGACGAAACACCGAACCCCTACCAACTGGT2306220.9172209139349833No Hit
GATCGCGCGGTTCTTCAGGAAAGGACGAAACACCGAACCCCTACCAACTG2219640.8827866506259793No Hit
TCGATCCTTACCATCAAGTGGAAAGGACGAAACACCGAACCCCTACCAAC1935940.769954582010082No Hit
TAAGTAGAGTCTTGTGCAAAGGACGAAACACCGAACCCCTACCAACTGGT1849450.7355561131535823No Hit
TAAGTAGAGTCTTGTGGAAAGGACGAAACACCGAACCCCTACCATCTGGT1464910.5826183490874661No Hit
TCGATCCTTACCATCTTGTGGAAAGGACGAAACACCGAACCCCTACCAAC1399550.5566236222466658No Hit
TAAGTAGAGTCTTGTGGAAAGGACGAAACACCGAACCCGTACCATCTGGT1096640.4361514266018246No Hit
CGATCAAGATCGTCTAGTGGAAAGGACGAAACACCGAACCCCTACCAACT1072660.4266141935901601No Hit
TAAGTAGAGTCTTGTGGAAAGGACGAAACACCGAACCCGTACCAACTGGT952900.37898370879128856No Hit
TAAGTAGAGTCTTGTGGAAAGGACGAAACACCGAACCGCTACCAACTGGT881690.3506623425377177No Hit
TCGATCCATACCATCAAGTGGAAAGGACGAAACACCGAACCCCTACCAAC860230.34212735419616974No Hit
CGATCAAGATCGTCTTGTGGAAAGGACGAAACACCGATCCCCTACCAACT819650.32598803327818204No Hit
ATACACGATCTCTTGAGGAAAGGACGAAACACCGAACCCCTACCAACTGG818300.32545111649061964No Hit
TAAGTAGAGTCTTGTGGAAAGGACGAAACACCGAACCGCTACCATCTGGT801590.3188052798090135No Hit
TCGATCCATACCATCTTGTGGAAAGGACGAAACACCGAACCCCTACCAAC764290.30397046782673304No Hit
TAAGTAGAGTCTTGTGGAAAGGACGAAACACCGAACCGGTACCATCTGGT737490.29331167530327146No Hit
CGATCAAGATCGTCTACTGGAAAGGACGAAACACCGATCCCCTACCAACT667250.26537609370446774No Hit
ATAAACGATCTCTTGTCGAAAGGACGAAACACCGAACCCCTACCAACTGG653050.25972852453158884No Hit
TCGATCCTTACCATCTAGTGGAAAGGACGATTCACCGATCCCCTTCCAAC632910.2517185214934352No Hit
GATAGCGCGGTTCTTGAGGAAAGGACGAAACACCGAACCCCTACCAACTG620820.24691013337370948No Hit
CGATCAAGATCGTCTTCTGGAAAGGACGAAACACCGATCCCCTACCAACT610830.24293694914574748No Hit
CGATCAAGATCGTCTAGTGGAAAGGACGAAACACCGATCCCCTACCAACT573760.2281936118754221No Hit
ATACACGATCTCTTGTGGAAAGGACGAAACTCCGAACCGCTACCTTCTGG545770.21706153714662774No Hit
GATAGCGCGGTTCTTGTGGAAAGGACGAAACACCGAACCCCTACCAACTG542410.21572521091980565No Hit
ATACACGATCTCTTGTCGAAAGGACGAAACTCCGAACCGCTACCTTCTGG535240.21287358620364077No Hit
ATAAACGATCTCTTGTGGAAAGGACGAAACACCGAACCCCTACCAACTGG517240.20571469570280837No Hit
GATCGCGCGGTTCTTGAGGAAAGGACGAAACTCCGAACCCCTACCTACTG496550.19748594878824044No Hit
TCGATCCTTACCATCTAGTGGAAAGGACGAAACACCGATCCCCTACCAAC481000.1913014628277991No Hit
CGATCAAGATCGTCTTGTGGAAAGGACGATTCACCGATCCCCTACCAACT476600.18955151181648455No Hit
GATCGCGCGGTTCTTGAGGAAAGGACGAATCTCCGAACGCCTACCTACTG473520.18832654610856434No Hit
CGATCAAGATCGTCTTCTGGAAAGGACGAATCACCGAACCCCTACCAACT472650.18798053306769077No Hit
TAAGTAGAGTCTTGTGGAAAGGACGAAACACCGAACCGGTACCAACTGGT463600.18438120201032782No Hit
GATCGGGCGGTTCTTGAGGAAAGGACGAAACTCCGAACGCCTACCTACTG458900.1825119361573327No Hit
GATCGGGCGGTTCTTGTGGAAAGGACGAAACTCCGAACGCCTACCTACTG456580.18158923471500318No Hit
GATCGCGCGGTTCTTGAGGAAAGGACGAAACTCCGAACCCCTACCAACTG454030.1805750585607186No Hit
GATCGCGCGGTTCTTGTGGAAAGGACGAAACTCCGAACCCCTACCTACTG447560.17800183514180828No Hit
GATCGCGCGGTTCTTGAGGAAAGGACGAAACTCCGAACGCCTACCTACTG434380.17275993643064322No Hit
CGATCAAGATCGTCTTGTGGAAAGGACGAATCACCGAACCCCTACCAACT431920.1717815547288628No Hit
CGATCATGATCGTCTTGTGGAAAGGACGAAACACCGAACCCCTACCAACT431270.17152303923855494No Hit
GATCGGGCGGTTCTTGAGGAAAGGACGAATCTCCGAACGCCTACCTACTG430530.17122872929574295No Hit
ATACACGATCTCTTGTGGAAAGGACGAAACACCGAACCGCTACCTTCTGG427790.17013898707506067No Hit
GATCGCGCGGTTCTTGAGGAAAGGACGAAACACCGAACCCCTACCTACTG414470.16484140810444473No Hit
CGATCAAGATCGTCTAGTGGAAAGGACGATTCACCGATCCCCTACCAACT413640.16451130370912856No Hit
TAAGTTGAGTCTTGTGGAAAGGACGAAACACCGAACCCCTACCAACTGGT391770.1558132517506172No Hit
ATAAACGATCTCTTGACGAAAGGACGAAACACCGAACCCCTACCAACTGG388320.15444113107129098No Hit
ATACACGATCTCTTGTCGAAAGGACGAAACACCGAACCCCTACCTTCTGG388130.1543655650048933No Hit
ATACACGATCTCTTGTGGAAAGGACGAAACACCGAACCGCTACCATCTGG387850.15425420448599148No Hit
TAAGTAGAGTCTTGTGGAAAGGACGAAACACCGAACCCCTACCAACAGGT379550.15095316053282984No Hit
TCGATCCTTACCATCTAGTGGAAAGGACGATTCACCGATCCCCTACCAAC372980.14834016550002602No Hit
CGATCAAGATCGTCTACTGGAAAGGACGAATCACCGAACCCCTACCAACT372290.14806574136416079No Hit
GATCGCGCGGTTCTTGAGGAAAGGACGAATCTCCGAACCCCTACCTACTG365730.1454567234927463No Hit
ATACACGATCTCTTGTGGAAAGGACGAAACACCGAACCGCTACCAACTGG359240.1428755457510573No Hit
TCGATCCATACCATCTAGTGGAAAGGACGATTCACCGATCCCCTACCAAC356670.141853415273994No Hit
TAAGTACAGTCTTGTGGAAAGGACGAAACACCGAACCCCTACCAACTGGT349100.13884270410225505No Hit
TCGATCCTTACCATCTTGTGGAAAGGACGAAACACCGATCCCCTACCAAC347870.13835351325136483No Hit
CGATCAAGATCGTCTTGTGGAAAGGACGAATCACCGATCCCCTACCAACT347160.1380711347927209No Hit
ATACACGATCTCTTGTCGAAAGGACGAAACACCGAACCCCTACCATCTGG342210.136102439904992No Hit
GATCGCGCGGTTCTTGTGGAAAGGACGAAACTCCGAACGCCTACCTACTG339880.13517576130127312No Hit
GATCGGGCGGTTCTTGAGGAAAGGACGAAACTCCGAACCCCTACCTACTG334310.13296048240740443No Hit
TAAGTAGAGTCTTGTGGAAAGGACGAAACTCCGAACCGGTACCATCTGGT332330.13217300445231286No Hit
TAAGTAGAGTCTTGTGGAAAGGACGAAACTCCGAACCCCTACCAACTGGT329860.1311906455891431No Hit
ATACACGATCTCTTGTGGAAAGGACGAAACACCGAACCCCTACCATCTGG329120.1308963356463311No Hit
CGATCAAGATCGTCTAGTGGAAAGGACGAATCACCGATCCCCTACCAACT327050.13007306323873535No Hit
TAAGTAGAGTCTTGTGGAAAGGACGAAACTCCGAACCCGTACCAACTGGT325500.1294566032233859No Hit
TCGATCCTTACCATCAAGTGGAAAGGACGATTCACCGATCCCCTTCCAAC323570.12868901107524108No Hit
GATCGCGCGGTTCTTGTGGAAAGGACGAAACTCCGAACCCCTACCAACTG322490.12825947764519113No Hit
TAAGTAGAGTCTTGTCCAAAGGACGAAACACCGAACCCCTACCAACTGGT318390.12662884147555709No Hit
CGATCAAGATCGTCTTGTGGAAAGGACGAAACACCGAACCCCTACGAACT314850.12522092634372672No Hit
CGATCAAGATCGTCTTCTGGAAAGGACGATTCACCGATCCCCTACCAACT314410.12504593124259528No Hit
ATACACGATCTCTTGTCGAAAGGACGAAACACCGAACCGCTACCAACTGG309030.12290621841512427No Hit
GATAGCGCGGTTCTTGAGGAAAGGACGAAACTCCGAACCCCTACCTACTG307920.12246475350090626No Hit
CGATCAAGATCGTCTTCTGGAAAGGACGATTCACCGAACCCCTACCAACT306910.12206306020058177No Hit
TCGATCCTTACCATCTTGTGGAAAGGACGATTCACCGATCCCCTACCAAC301940.12008641099007415No Hit
TCGATCCTTACCATCTTGTGGAAAGGACGATTCACCGATCCCCTTCCAAC300980.11970460349669643No Hit
TAAGTAGAGTCTTGTCGAAAGGACGAAACACCGAACCCCTACCATCTGGT297220.11820919081430031No Hit
ATACACGATCTCTTGTGGAAAGGACGAAACTCCGAACCGCTACCATCTGG293050.11655071451494083No Hit
GATAGCGCGGTTCTTCAGGAAAGGACGAAACACCGAACCCCTACCAACTG293050.11655071451494083No Hit
CGATAAAGATCGTCTACTGGAAAGGACGAAACACCGAACCCCTACCAACT291470.11592232301542331No Hit
CGATCATGATCGTCTTCTGGAAAGGACGAAACACCGAACCCCTACCAACT288070.11457008814304386No Hit
TAAGTTGAGTCTTGTGGAAAGGACGAAACACCGAACCCGTACCATCTGGT284180.11302297236258618No Hit
ATACACCATCTCTTGTCGAAAGGACGAAACACCGAACCCCTACCAACTGG281570.11198493323996549No Hit
GATCGCGCGGTTCTTGTGGAAAGGACGAATCTCCGAACGCCTACCTACTG279150.11102246018374247No Hit
CGATCAAGATCGTCTTGTGGAAAGGACGAAACACCGATCCCCTACGAACT278310.11068837862703695No Hit
CGATCAAGATCGTCTTGTGGAAAGGACGATTCACCGAACCCCTACCAACT276090.10980544879860096No Hit
TCGATCCTTACCATCTAGTGGAAAGGACGAATCACCGATCCCCTACCAAC275950.10974976853915006No Hit
TCGATCGTTACCATCTTGTGGAAAGGACGAAACACCGATCCCCTACCAAC271880.10813106385368404No Hit
ATACAGGATCTCTTGTGGAAAGGACGAAACTCCGAACCGCTACCATCTGG270400.10754244396806005No Hit
CGATCTAGATCGTCTTGTGGAAAGGACGAAACACCGAACCCCTACCAACT269180.10705723027855918No Hit
GATCGCGCGGTTCTTGAGGAAAGGACGAAACTCCGAACGCCTACGTACTG267850.10652826781377546No Hit
ATACACGATCTCTTGTGGAAAGGACGAAACTCCGAACCGCTACCAACTGG267120.10623793503235282No Hit
TAAGTTGAGTCTTGTGGAAAGGACGAAACACCGAACCGGTACCATCTGGT267000.10619020909568061No Hit
ATACACGATCTCTTGTCGAAAGGACGAAACACCGAACCGCTACCTTCTGG266200.10587203618453249No Hit
ATACAGGATCTCTTGTGGAAAGGACGAAACACCGAACCGCTACCATCTGG264620.10524364468501499No Hit
GATCGCGCGGTTCTTGTGGAAAGGACGAATCTCCGAACCCCTACCTACTG264490.10519194158695343No Hit
GATCGGGCGGTTCTTGAGGAAAGGACGAAACTCCGAACGCCTACGTACTG264060.1050209236472113No Hit
ATACACCATCTCTTGACGAAAGGACGAAACACCGAACCCCTACCAACTGG263890.10495331190359233No Hit
TAAGTAGAGTCTTGTGGAAAGGACGAAACTCCGAACCGGTACCAACTGGT263570.1048260427391331No Hit
TAAGTTGAGTCTTGTGGAAAGGACGAAACACCGAACCCGTACCAACTGGT261690.10407833639793504No Hit
ATACACGATCTCTTGTCGAAAGGACGAAACACCGAACCGCTACCATCTGG259710.10329085844284348No Hit
ATACAGGATCTCTTGAGGAAAGGACGAAACTCCGAACCGCTACCATCTGG259100.10304825159809304No Hit
ATACACGATCTCTTGTGGAAAGGACGAAACACCGAACCCCTACCTTCTGG256030.10182726305156219No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACACGA3579850.0144.623952
GATCGCG2550450.0144.618091
GATCAAG4346550.0144.372472
TAGAGTC3559000.0144.307345
GATCGTC5923500.0144.229458
TAAGTAG3544800.0144.148571
CACGATC3579150.0144.049294
GCGGTTC3966550.0144.023917
TCCTTAC2346850.0144.01465
CCTTACC2348350.0143.94116
GTAGAGT3563700.0143.940224
AAGTAGA3561400.0143.937622
TCTCTTG5785150.0143.934199
ATCAAGA4371650.0143.901353
ATCGTCT5925300.0143.89859
TTACCAT3454300.0143.822688
AGATCGT5240350.0143.76397
CTTACCA2801650.0143.729027
TCGCGCG2584500.0143.691913
ATCCTTA2350950.0143.67414
Produced by FastQC (version 0.11.5)