FastQCFastQC Report
Tue 8 Oct 2019
HVNVGAFXY_n01_PCR2_R3_rc.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHVNVGAFXY_n01_PCR2_R3_rc.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences85
Sequences flagged as poor quality0
Sequence length151
%GC50

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGCGCGGTTCTTGAGGAAAGGACGAAACACCGAACCCCTACCAACTG44.705882352941177No Hit
CGATCAAGATCGTCTACTGGAAAGGACGAAACACCGAACCCCTACCAACT33.5294117647058822No Hit
CGATCAAGATCGTCTTCTGGAAAGGACGAAACACCGAACCCCTACCAACT33.5294117647058822No Hit
ATAAACGATCTCTTGTCGAAAGGACGAAACACCGAACCCCTACCAACTGG22.3529411764705883No Hit
CGATCAAGATCGTCTACTGGAAAGGACGAAACACCGATCCCCTACCAACT11.1764705882352942No Hit
GATCGCGCGGTTCTTGAGGAAAGGACGAAACTCCGAACCCCCACCAACTG11.1764705882352942No Hit
TCGATCCTTACCATCTTGTGGAAAGGACGAAACACCGATCCCCTACCAAC11.1764705882352942No Hit
ATACAGGATCTCTTGAGGCAAGGACGAAACTGCGAACCGCTACCTTCTGG11.1764705882352942No Hit
CGATCAAGATCGTCTACTGGAAAGGACGAAAGACCGATGCCCTAGGAACT11.1764705882352942No Hit
CGATCAAGATCGTCTTGTGGAAAGGACGAAACACCGATCCCCTACGAACT11.1764705882352942No Hit
TAAGTAGAGTCTTGTGGAAAGGACGAAACACCGAACCCCTACCAACTGGT11.1764705882352942No Hit
ATACACGATCTCTTGAGGAAAGGACGAAACTCCGAACCCCTACCTACTGG11.1764705882352942No Hit
CGATCAAGATCGTCTTGTGGAAAGGACGAAACACCGATCGCCTACGAACT11.1764705882352942No Hit
TCGATGGTTACCATCAAGTGGAAAGGACGATTCACCGTTCCCCTTCCAAC11.1764705882352942No Hit
ATACACGATCTCTTGTGGAAAGGACGAAACACCGAACCGGTACCATCTGG11.1764705882352942No Hit
TAAGTACAGTCTTGAGCAAAGGACGAAACACCGAACCCGTACCAACTGGT11.1764705882352942No Hit
GATAGCCCGGTTCTTGAGGAAAGGACGAATCTCCGAACCCCTACGTACTG11.1764705882352942No Hit
ATACACGATCTCTTGTGGAAAGGACGAAACACCGATCCGCTACCTTCTGG11.1764705882352942No Hit
TAAGTTCAGTCTTGTGGAAAGGACGAAACACCGAACCCGTACCAACTGGT11.1764705882352942No Hit
ATACAGGATCTCTTGTCGAAAGGACGAAACAGCGTACCGCTACCTTCTGG11.1764705882352942No Hit
GATCGCGCGGTTCTTGAGGAAAGGACGAATCTCCGAAGGCCTACCAACTG11.1764705882352942No Hit
GATCGGCCGGTTCTTGTGGAAAGGACGAAACACCGAACGCCTACGTACTG11.1764705882352942No Hit
TCGATCGTTACCATCTAGTGGAAAGGACGAATCACCGATCCCCTACCAAC11.1764705882352942No Hit
TCGATCCTTACCATCTTGTGGAAAGGACGATTCACCGATCCCCTTCCAAA11.1764705882352942No Hit
CGATCAAGATCGTCTTGTGGAAAGGACGAATCACCGAACCCCTACCAACT11.1764705882352942No Hit
GATCGGGCGGTTCTTGAGGAAAGGACGAAACACCGAACCCCTACCTACTG11.1764705882352942No Hit
GATCGCGCGGTTCTTGAGGAAAGGACGAAACACCGAACCCCTACCTACTG11.1764705882352942No Hit
CGATCATGATCGTCTTGTGGAAAGGACGAAACACCGAACCCCTACCAACT11.1764705882352942No Hit
ATACACGATCTCTTGTGGCAAGGACGAAACTCCGAACCGCTACCTTCTGG11.1764705882352942No Hit
GATCGCGCGGTTCTTGTGGAAAGGACGAATCTCCGNACCCCTACCAACTG11.1764705882352942No Hit
ATACAGGATCTCTTGTGGAAAGGACGAAACTCCGAACGCCTACCATCTGG11.1764705882352942No Hit
GATCGGGCGGTTCTTGAGGAAAGGACGAAACACCGAACCCCTACCAACTG11.1764705882352942No Hit
GATAGCGCGGTTCTTCAGGAAAGGACGAAACACCGAACCCCTACCAACTG11.1764705882352942No Hit
ATACACGATCTCTTGTCGAAAGGACGAAACACCGAACCGCTACCAACTGG11.1764705882352942No Hit
CGATCATGATCGTCTTCTGGAAAGGACGAAACACCGAACCCCTACCAACT11.1764705882352942No Hit
ATACACGATCTCTTGTGGAAAGGACGAAACACCGAACCGCTACCAACTGG11.1764705882352942No Hit
CGATCAAGATCGTCTACTGGAAAGGACGAATCACCGAACCCCTACCAACT11.1764705882352942No Hit
ATAGACGATCTCTTGACGAAAGGACGAAACGCCGAACCCCTACCTACTGG11.1764705882352942No Hit
ATACACGATCTCTTGACGAAAGGACGAAACTCCGAACCGCTACCTTCTGG11.1764705882352942No Hit
CGATCAAGATCGTCTACTGGAAAGGACGAAACACCGATCCCCTACGAACT11.1764705882352942No Hit
CGATCATGATCGTCTAGTGGAAAGGACGAAAGACCGATCCCCTACCAACT11.1764705882352942No Hit
TAAGTACAGTCTTGTGGAAAGGACGAAACTCCGAACCCCTACCATCTGGT11.1764705882352942No Hit
GATCGCGCGGTTCTTCAGGAAAGGACGAAACACCGAACCCCTACCAACTG11.1764705882352942No Hit
CGATCAAGATCGTCTAGTCCAAAGGACGAAACACCGAACCCCTACCAACT11.1764705882352942No Hit
CGATCTAGATCGTCTTGTGGAAAGGACGAAAGACCGAACCCCTACCAACT11.1764705882352942No Hit
GATCGGGCGGTTCTTGAGGAAAGGACGAAACACCGAACGCCTACGTACTG11.1764705882352942No Hit
GATCGCCCGGTTCTTGTGGAAAGGACGAAACACCGATCGCCTACGTACTG11.1764705882352942No Hit
GGGGGCGATCTCGTGTGGAAAGGCCGAAACACCGAACCGCTACCAACTGG11.1764705882352942No Hit
TCGATCCATACCATCTAGTGGAAAGGACGAAACACCGAACCCCTACCAAC11.1764705882352942No Hit
GATAGCGCGGTTCTTGAGGAAAGGACGAAACACCGAACCCCTACCAACTG11.1764705882352942No Hit
GATCGCGCGGTTCTTGTGGAAAGGACGAAACACCGAACCCCTACCAACTG11.1764705882352942No Hit
GATCGCGCGGTTCTTGTGGAAAGGACGAAACTCCGAACGCCTACCTACTG11.1764705882352942No Hit
TAAGTAGAGTCTAGTGCAAAGGACGAAACACCGAACCCCTACCAACTGGT11.1764705882352942No Hit
CGATCAAGATCGACTTCTGGAAAGGACGAAACACCGAACCCCTACCAACT11.1764705882352942No Hit
CGATATAGATCGTCTTGTGGAAAGGACGAAACACCGAACCCCTACCAACT11.1764705882352942No Hit
TAAGTAGAGTCTTGTCGAAAGGACGAAACACCGAACCCCTACCAACTGGT11.1764705882352942No Hit
ATACACGATCTCTTGTCGAAAGGACGAAACACCGAACCCCTACCAACTGG11.1764705882352942No Hit
ATACACGATCTCTTGACGAAAGGACGAAACACCGAACCGCTACCATCTGG11.1764705882352942No Hit
TAAGTTGAGTCTTGTGCAAAGGACGAAACACCGAACCCATACCATCTGGT11.1764705882352942No Hit
ATACAGGATCTCTTGAGGAAAGGACGAAACTGCGAACGGCTACCTTCTGG11.1764705882352942No Hit
TAAGTAGAGTCTTGTGCAAAGGACGAAACACCGAACCCCTACCATCTGGT11.1764705882352942No Hit
CGATCAAGATCGTCTTCTGGAAAGGACGAACCACCGAACCCCTACCAACT11.1764705882352942No Hit
CGATCTAGATCGTCTTCTGGAAAGGACGATTCACCGATCCCCTACCTACT11.1764705882352942No Hit
ATAAACCATCTCTTGTCGAAAGGACGAAACACCGAACCGCTACCTTCTGG11.1764705882352942No Hit
ATACACGATCTCTTGTGGAAAGGACGAAACACCGATCCGCTACCATCTGG11.1764705882352942No Hit
TAAGTAGAGTCTTGTGGAAAGGACGAAACACCGAACCCGTACCAACTGGT11.1764705882352942No Hit
TAAGTAGAGTCTTGAGGAAAGGACGAAACACCGAACCCCTACCAACTGGT11.1764705882352942No Hit
GGGGGGCGGGGGGGGCCGGGGGGGGGGGGGGTGGGGGGGGGGGGGGGGGG11.1764705882352942No Hit
CGATCTAGATCGTCTTCTGGAAAGGACGAATGACCGATCCCCTAGCAACT11.1764705882352942No Hit
ATACACGATCTCTTGTGGAAAGGACGAAACACCGAACCCCTACCAACTGG11.1764705882352942No Hit
TCGATCCTTACCATCTAGTGGAAAGGACGAAACACCGAACCCCTACCAAC11.1764705882352942No Hit
CGATCATGATCGTCTTCTGGAAAGGACGAATGACCGATCCCCTACGAACT11.1764705882352942No Hit
ATAGACCATCTCTTGAGGAAAGGACGAAACACCGAACGCCTACCATCTGG11.1764705882352942No Hit
TCGATCCTTACCATCAAGTGGAAAGGTCGAATCACCGTTCCCCTAGCAAC11.1764705882352942No Hit
TAAGTAGAGTCTTGTGGAAAGGACGAAACACCGAACCCCTACCATCTGGT11.1764705882352942No Hit
ATACAGGATCTCTTGAGGAAAGGACGAAACACCGAACCGCTACCAACAGG11.1764705882352942No Hit
ATACAGCATCTCTTGTGGAAAGGACGAAACACCGATCCGCTACCTTCTGG11.1764705882352942No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGCGCG50.0145.03
GCGGTTC50.0145.07
GGTTCTT50.0145.09
GATCGGG50.0145.0145
GCGCGGT50.0145.05
ATAGCGC50.0145.02
CGCGGTT50.0145.06
AGCGCGG50.0145.04
CGGTTCT50.0145.08
GATAGCG50.0145.01
Produced by FastQC (version 0.11.5)