FastQCFastQC Report
Wed 14 Dec 2016
HVMVCBGXY_n02_brugia6-l3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHVMVCBGXY_n02_brugia6-l3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences76568470
Sequences flagged as poor quality0
Sequence length151
%GC44

[WARN]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTTAGTTTTACCCTACTGTGATATGTTGTTGCGATAGTAATCCTGCTCAG10285931.3433636586965887No Hit
CTGCAAGTGATCTTGCGCCCCGACATTGTACCAGAGTAATCCGGTTTTGG7790311.0174305428853418No Hit
GTTTTACCCTACTGTGATATGTTGTTGCGATAGTAATCCTGCTCAGTACG7019510.9167624741620146No Hit
CCGACATCGAAGGATCAAAAAGCAACGTCGCTATGAACGCTTGGCTGCCA4731140.6178966355211224No Hit
GCCTTGTCTAGGTGGGATTCTGACTTAGAGGCGTTCAGTCATAATCCCTC2784840.3637058439328878No Hit
GCGGTGTGTACAAAGGGCAGGGACGTAATCAACGTGAGCTGATGACTCAC2473250.32301154770364354No Hit
CTCACCTGGCCCGGACACCGTGAGGATTGACAGATTGAGAGCTCTTTCAT2410080.31476141550170716No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG2314270.3022484320243045No Hit
GCAAGTGATCTTGCGCCCCGACATTGTACCAGAGTAATCCGGTTTTGGCA2280220.2978014318426371No Hit
CGCGCGTGCAGCCCTGGACATCTAAGGGCATCACAGACCTGTTATTGCTC2162730.2824569956798144No Hit
GTCGTCTGCAAGTGATCTTGCGCCCCGACATTGTACCAGAGTAATCCGGT2095500.27367661910966745No Hit
AAGTGATCTTGCGCCCCGACATTGTACCAGAGTAATCCGGTTTTGGCAAC2066370.2698721810687872No Hit
GTCTGCAAGTGATCTTGCGCCCCGACATTGTACCAGAGTAATCCGGTTTT2033200.26554011070091904No Hit
GCGATAGTAATCCTGCTCAGTACGAGAGGAACCGCAGGTTCAGACATTTG2023260.2642419262132311No Hit
GCCAGTTCTAAGTTGACTGTTTAACGCCGGCCGAAATATCAAATAAAACA1981300.258761863727981No Hit
GTGATTTGTCTGGTTTATTCCGATAACGAGCGAGACTCTAGCCTACTAAA1898120.2478983842827211No Hit
TGGAAATCAAGATCAAGCGAGCTTTTGCCCTTTTACTCTACGAGAGGTTT1891300.24700767822577624No Hit
CTAGCATATACGCAAGTTATTGGGTAGTAAACCTAATTTAAAAGATAAAA1888280.2466132600011467No Hit
CTTGTCTAGGTGGGATTCTGACTTAGAGGCGTTCAGTCATAATCCCTCAG1871290.24439433098245267No Hit
GGCATTAGCTGTAATAGTATGAATATGTTCATAATTACATAATAGTGCCT1785000.23312467912706106No Hit
GTGATCTTGCGCCCCGACATTGTACCAGAGTAATCCGGTTTTGGCAACCA1736370.2267735008940364No Hit
GTTGCGATAGTAATCCTGCTCAGTACGAGAGGAACCGCAGGTTCAGACAT1659410.21672236626903998No Hit
GACAGGTTAGTTTTACCCTACTGTGATATGTTGTTGCGATAGTAATCCTG1618740.2114107804426548No Hit
GTACAAAGGGCAGGGACGTAATCAACGTGAGCTGATGACTCACACTTACT1505170.19657830435948373No Hit
CAGAAATCAGAGACAGTCAACGCCCGCAAGGGCCATCTGTCTCCTCTGTT1501410.19608724060961388No Hit
CTGTAATAGTATGAATATGTTCATAATTACATAATAGTGCCTCCCGACAT1487320.19424705756821312No Hit
GGCGAATTCTGCTTCGCAATGATAGGAAGAGCCGACATCGAAGGATCAAA1481750.19351960408768779No Hit
CTGACTTAGAGGCGTTCAGTCATAATCCCTCAGATGGTAGCTTCGCACCA1398230.18261171994164177No Hit
AGACAGGTTAGTTTTACCCTACTGTGATATGTTGTTGCGATAGTAATCCT1383140.18064093483910545No Hit
CAAAGGGCAGGGACGTAATCAACGTGAGCTGATGACTCACACTTACTAGG1378780.18007150985255418No Hit
CCCGAGTCCGTGCCAGTTCTAAGTTGACTGTTTAACGCCGGCCGAAATAT1360740.17771544866966782No Hit
GCGAAACCACAGCCAAGGGAACGGGCTTGGCAAACACAGCGGGGAAAGAA1313390.17153144107489676No Hit
CGTCTGCAAGTGATCTTGCGCCCCGACATTGTACCAGAGTAATCCGGTTT1289700.1684374782465942No Hit
CTCCAGTGTAGCGCGCGTGCAGCCCTGGACATCTAAGGGCATCACAGACC1286730.16804959012502144No Hit
CGGAAACCTCTCGTAGAGTAAAAGGGCAAAAGCTCGCTTGATCTTGATTT1283120.16757811668432188No Hit
GGGGCATTAGCTGTAATAGTATGAATATGTTCATAATTACATAATAGTGC1206110.15752045195626868No Hit
CTGGAAATCAAGATCAAGCGAGCTTTTGCCCTTTTACTCTACGAGAGGTT1204330.1572879802874473No Hit
CTGGCCCGGACACCGTGAGGATTGACAGATTGAGAGCTCTTTCATGATTC1201490.1569170704338222No Hit
CCCGTCCGTCCCTCTTAACCATTATCTCAGATCAGAAAACCAACAAAATA1167840.15252231107660894No Hit
TGCAAGTGATCTTGCGCCCCGACATTGTACCAGAGTAATCCGGTTTTGGC1161730.15172433248307038No Hit
GCGAGTCGTTAAATCACCGAGTGATTTAACTTAAATCAAAAATTTCGAAA1151930.15044443228394141No Hit
GGAGTCTAGCATATACGCAAGTTATTGGGTAGTAAACCTAATTTAAAAGA1143170.1493003582283935No Hit
GGAAATCAAGATCAAGCGAGCTTTTGCCCTTTTACTCTACGAGAGGTTTC1140030.1488902677564277No Hit
GTCAAAACGTAACGCAGGTGTCCTAAGGCTAGCTCAGAGAGGACGGAAAC1134480.14816542631712504No Hit
GCCTGACACTGTCTTCGGTGCGAGTCGTTAAATCACCGAGTGATTTAACT1123310.1467066012942403No Hit
CAGCTTTGCAACCATACTTCCCCCGGAACCGAAAAACTTTCGTTTCCGGG1107690.1446665970993021No Hit
CCCGAAGTTACGGATCTAATTTGCCGACTTCCCTTACCTACATTATTCTA1099000.14353166518803367No Hit
CCCCAGTCAAACTCCCCGCCTGACACTGTCTTCGGTGCGAGTCGTTAAAT1094220.14290738733580546No Hit
CTCCACTCCTGGTGGTGCCCTTCCGTCAATTTCTTTAAGTTTCAGCTTTG1088060.14210287863921012No Hit
GGCGGTGTGTACAAAGGGCAGGGACGTAATCAACGTGAGCTGATGACTCA1054340.1376989771377174No Hit
GCGAGACTCTAGCCTACTAAATAGTTACTGGATAATTTTATCGTCCAGAC1034920.13516268511046386No Hit
CTGGTTTATTCCGATAACGAGCGAGACTCTAGCCTACTAAATAGTTACTG1019200.1331096207094121No Hit
GTGTACAAAGGGCAGGGACGTAATCAACGTGAGCTGATGACTCACACTTA996790.1301828285193631No Hit
GCGCGTGCAGCCCTGGACATCTAAGGGCATCACAGACCTGTTATTGCTCA984270.1285476907139453No Hit
CGCCCCTATACCAAAGTCAGACGATCGATTTGCACGTCAGAATCGCTTCG982540.1283217491481807No Hit
GTCAAACTCCCCGCCTGACACTGTCTTCGGTGCGAGTCGTTAAATCACCG976300.1275067922866945No Hit
GCAAGTTATTGGGTAGTAAACCTAATTTAAAAGATAAAAAGCATAATGAA976180.12749112003935825No Hit
CTGCTCTACTGCTTACAACACCTTGACCAGTAATCAAGTCGTCTGCAAGT946520.1236174629060761No Hit
CTTGATTAATGAAGACATTCTTGGCAAATGCTTTCGCAGTCGTACGTCTC941830.12300493923935009No Hit
GTTTTAATTAGACAGTCGGATTCCCCGAGTCCGTGCCAGTTCTAAGTTGA927070.12107725281698851No Hit
GTCTAGGTGGGATTCTGACTTAGAGGCGTTCAGTCATAATCCCTCAGATG922780.12051696997471674No Hit
CTCGTTAACAGGTTTAAATTGTACCCATTCCGATAACCGGCCTCAAAGAG917260.11979604659724818No Hit
CAGGGACGTAATCAACGTGAGCTGATGACTCACACTTACTAGGAATTCCT909070.11872641571654755No Hit
CCCGCCTGACACTGTCTTCGGTGCGAGTCGTTAAATCACCGAGTGATTTA906180.11834897575986565No Hit
GATTTGTCTGGTTTATTCCGATAACGAGCGAGACTCTAGCCTACTAAATA900800.11764633667095607No Hit
CTCTGTTTTAATTAGACAGTCGGATTCCCCGAGTCCGTGCCAGTTCTAAG899100.11742431316702553No Hit
CGAGAATTGAAATATTATATGTATTTCGAAATTTTTGATTTAAGTTAAAT893190.11665245498571408No Hit
CTTGTTACGACTTTTGCCCGGTTCAAGCCACTGCGATTAAAGCAGTTTCC892240.11652838302763527No Hit
GGCTGCTCTACTGCTTACAACACCTTGACCAGTAATCAAGTCGTCTGCAA892190.11652185292457848No Hit
GCCGTTCTTAGTTGGTGGAGTGATTTGTCTGGTTTATTCCGATAACGAGC891380.11641606525505865No Hit
CTTGACTCTAGTCCGACTCTGTGAAGAGTCATGAGAGGTGTAGCATAAGT882350.1152367286430041No Hit
CTCCGTGTCCAGGTACGGGAATATTAACCCGTTTCCCTTTCGATTTATCA878110.11468297590378912No Hit
TAGTAATCCTGCTCAGTACGAGAGGAACCGCAGGTTCAGACATTTGGTTC864190.11286499521278145No Hit
GGCGGGAGTAACTATGACTCTCTTAAGGTAGCCAAATGCCTCGTCATCTA824740.1077127439009817No Hit
GACAGATTGAGAGCTCTTTCATGATTCGGTGGTTGGTGGTGCATGGCCGT814450.10636884869189629No Hit
GTGTGTACAAAGGGCAGGGACGTAATCAACGTGAGCTGATGACTCACACT790090.10318738248263286No Hit
GCCGACATCGAAGGATCAAAAAGCAACGTCGCTATGAACGCTTGGCTGCC780450.1019283786132856No Hit
GTGAAATACCACTACTTTCATCGATTCTTTACTTATTCGGTAAAACGAGA778000.10160840356350336No Hit
GTCCAATCAAAGGATAAGTCTCAACAGATCGCAGTATGGAGGCTGCTCTA771070.1007033312798336No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGACAA312500.094.22085145
GTTAGTT2011300.079.789211
CACCTGG486900.079.67243
ACCTGGC489100.079.5316544
TTAGTTT2153950.074.47152
GGAGTCT244750.074.226781
CCTGGCC526400.074.028245
GTCCAAT166150.073.999321
AGTTTTA2240150.072.181934
GAGTCTA254700.071.44372
TAGTTTT2254250.071.361213
CTTGTTA194700.071.079151
TCCAATC181850.067.9051062
AGTCTAG292400.062.3072173
CTGGCCC676000.057.766216
CTGCAAG2200500.056.6280331
TACCCTA2875350.055.9171879
TTACCCT2871500.055.847958
GTTACGA252650.054.648944
GCCCGGA716800.054.5058829
Produced by FastQC (version 0.11.2)