FastQCFastQC Report
Wed 14 Dec 2016
HVMVCBGXY_n02_brugia4-l3d6.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHVMVCBGXY_n02_brugia4-l3d6.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences74980141
Sequences flagged as poor quality0
Sequence length151
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTTAGTTTTACCCTACTGTGATATGTTGTTGCGATAGTAATCCTGCTCAG8047171.0732401796897126No Hit
GCCTTGTCTAGGTGGGATTCTGACTTAGAGGCGTTCAGTCATAATCCCTC7727911.0306609052655689No Hit
GCCCTCTTGAACTCTCTCTTCAAAGTTCTTTGCAACTTTCCCTCACGGTA6496570.8664387547630779No Hit
GTTTTACCCTACTGTGATATGTTGTTGCGATAGTAATCCTGCTCAGTACG5556780.7411002334604838No Hit
CTTGTCTAGGTGGGATTCTGACTTAGAGGCGTTCAGTCATAATCCCTCAG4375960.5836158670333789No Hit
CTCCAGTGTAGCGCGCGTGCAGCCCTGGACATCTAAGGGCATCACAGACC3618860.4826424639558893No Hit
CTCGTTAACAGGTTTAAATTGTACCCATTCCGATAACCGGCCTCAAAGAG3273430.4365729320247611No Hit
CTCCGTGTCCAGGTACGGGAATATTAACCCGTTTCCCTTTCGATTTATCA2998630.3999232276717111No Hit
GAAAAAAGTTACGGTAGGTTATTCCTTGGAGTCGGGTTGTTTGAGATTGC2945920.3928933662581403No Hit
CTGCAAGTGATCTTGCGCCCCGACATTGTACCAGAGTAATCCGGTTTTGG2832700.3777933679799295No Hit
GCGCCTGCTGCCTTCCTTGGATGTGGTAGCCGTTTCTCAGGCTCCCTCTC2677890.35714656764915925No Hit
CTCTGAATAGCTATGGCTGATCGCATGGTCTTGTACCGGCGACGTATCTA2625740.3501913926782293No Hit
AAAAAAGTTACGGTAGGTTATTCCTTGGAGTCGGGTTGTTTGAGATTGCA2484350.3313343995978882No Hit
CTGAATAGCTATGGCTGATCGCATGGTCTTGTACCGGCGACGTATCTATC2446390.3262717257360186No Hit
CCCGTCCGTCCCTCTTAACCATTATCTCAGATCAGAAAACCAACAAAATA2443660.3259076293281443No Hit
CGCGCCTGCTGCCTTCCTTGGATGTGGTAGCCGTTTCTCAGGCTCCCTCT2374750.3167171958238916No Hit
CTCACCTGGCCCGGACACCGTGAGGATTGACAGATTGAGAGCTCTTTCAT2068240.2758383716563029No Hit
GCTAGACCTGTACGGGTGAAAAAAGTTACGGTAGGTTATTCCTTGGAGTC2055110.27408724131366996No Hit
GCGGATATGGGTACGATCTGGCACGAAATTCAAATAGCTTCCCTCGGATT2053280.27384317668861147No Hit
CTCGCTCGTTATCGGAATAAACCAGACAAATCACTCCACCAACTAAGAAC2041360.27225342240954176No Hit
CGCCCTCTTGAACTCTCTCTTCAAAGTTCTTTGCAACTTTCCCTCACGGT1963670.26189201217959834No Hit
CCTCGTTAACAGGTTTAAATTGTACCCATTCCGATAACCGGCCTCAAAGA1929750.25736814765392346No Hit
CGCGCGTGCAGCCCTGGACATCTAAGGGCATCACAGACCTGTTATTGCTC1910710.25482880860413426No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG1883130.25115050130407196No Hit
CTCTCTTCAAAGTTCTTTGCAACTTTCCCTCACGGTACTTGTTTGCTATC1870770.24950206482007015No Hit
CCCCAGTCAAACTCCCCGCCTGACACTGTCTTCGGTGCGAGTCGTTAAAT1741830.23230551140201242No Hit
CTCTTAACCATTATCTCAGATCAGAAAACCAACAAAATAGAACCGAGATC1740880.23217881118681813No Hit
GCTACTACCACCAAGATCTGCACCAATGGAAGCTTCAACCGGGCCTACGC1736230.23155864697560385No Hit
GTCTAGGTGGGATTCTGACTTAGAGGCGTTCAGTCATAATCCCTCAGATG1702720.2270894635954339No Hit
GTGACTCTGAATAGCTATGGCTGATCGCATGGTCTTGTACCGGCGACGTA1692020.2256624190664032No Hit
GCGAGTCGTTAAATCACCGAGTGATTTAACTTAAATCAAAAATTTCGAAA1673120.22314175162727423No Hit
AAAAAGTTACGGTAGGTTATTCCTTGGAGTCGGGTTGTTTGAGATTGCAG1660090.2214039581493985No Hit
CTCCACTTCAGTCTTCAAGGATCTCACTTGAATATTTGCTACTACCACCA1619340.21596918576080032No Hit
CTTGAACTCTCTCTTCAAAGTTCTTTGCAACTTTCCCTCACGGTACTTGT1616120.21553973871561538No Hit
CAAACGTTGAGTTAAAGTGCCAAACTTTCCGCTCATGAGACCCCATGAAA1538640.20520633590166226No Hit
CGGAAACCTCTCGTAGAGTAAAAGGGCAAAAGCTCGCTTGATCTTGATTT1490700.19881264293701448No Hit
CTCCACTCCTGGTGGTGCCCTTCCGTCAATTTCTTTAAGTTTCAGCTTTG1451820.19362726991937773No Hit
GCACCTTCTATGGACTGTAGCCCCGCTCAAGGGGACTTTGGGTGTAAAAA1425670.19013967978534477No Hit
ATTAAAACCAATCGGATTATTAATTTTAATCCGTTAATTGGTGACTCTGA1346390.17956621340576032No Hit
GTACGGGTGAAAAAAGTTACGGTAGGTTATTCCTTGGAGTCGGGTTGTTT1316770.1756158340646492No Hit
CTCCGTTACCCGTTACAACCATGGTAGGCACATAAACTACCATCGAAAGT1292320.17235497063149027No Hit
CGCCTGCTGCCTTCCTTGGATGTGGTAGCCGTTTCTCAGGCTCCCTCTCC1288330.17182282972767415No Hit
CAGGGACGTAATCAACGTGAGCTGATGACTCACACTTACTAGGAATTCCT1255610.16745900757908685No Hit
GACAGATTGAGAGCTCTTTCATGATTCGGTGGTTGGTGGTGCATGGCCGT1254450.16730429994790222No Hit
GTGGCTAGCAAGTTTACCTTGAAAAAATTAGAGTGCTCAATGCGGGCTAA1233050.16445021088984083No Hit
GCGCGCCTGCTGCCTTCCTTGGATGTGGTAGCCGTTTCTCAGGCTCCCTC1218620.16252570130536298No Hit
CTTGATTAATGAAGACATTCTTGGCAAATGCTTTCGCAGTCGTACGTCTC1161800.1549476947502673No Hit
GTGAGGATTGACAGATTGAGAGCTCTTTCATGATTCGGTGGTTGGTGGTG1152300.15368069259832415No Hit
CCCGCATTGAGCACTCTAATTTTTTCAAGGTAAACTTGCTAGCCACCATA1148500.15317389173754686No Hit
GTCGTCTGCAAGTGATCTTGCGCCCCGACATTGTACCAGAGTAATCCGGT1137800.1517468472085162No Hit
GACAGGTTAGTTTTACCCTACTGTGATATGTTGTTGCGATAGTAATCCTG1136390.15155879741543832No Hit
CTACCACCAAGATCTGCACCAATGGAAGCTTCAACCGGGCCTACGCCCAA1106850.147619087566133No Hit
GGCTGATCGCATGGTCTTGTACCGGCGACGTATCTATCAAGTGTCTGCCT1100870.1468215430536467No Hit
CCCTGTGGTAACTTTTCTGACACCTCTTGCTAGAAACCTTCTAAAAAATT1087770.1450744137704409No Hit
CCTTATTCTCCGTTACCCGTTACAACCATGGTAGGCACATAAACTACCAT1083500.14450492964530434No Hit
GTTACGGTAGGTTATTCCTTGGAGTCGGGTTGTTTGAGATTGCAGCCCAA1060480.14143478337817478No Hit
GCGGTGTGTACAAAGGGCAGGGACGTAATCAACGTGAGCTGATGACTCAC1057040.14097599523052376No Hit
GTACGATCTGGCACGAAATTCAAATAGCTTCCCTCGGATTTTCATGGATC1052550.1403771700029212No Hit
GTCCCTCTTAACCATTATCTCAGATCAGAAAACCAACAAAATAGAACCGA1045860.13948493375065804No Hit
ATTAGATTAAAACCAATCGGATTATTAATTTTAATCCGTTAATTGGTGAC1026810.13694426101439314No Hit
CTAAGAAGTTGTCTGGACGATAAAATTATCCAGTAACTATTTAGTAGGCT1019150.13592265717398425No Hit
CCGACATCGAAGGATCAAAAAGCAACGTCGCTATGAACGCTTGGCTGCCA1012370.13501841774343956No Hit
AAAGAAACTAACGAGGATTTCCCTAGTAACGGCGAGTGAAAAGGAAAAAG1007470.13436491137033205No Hit
AGACAGGTTAGTTTTACCCTACTGTGATATGTTGTTGCGATAGTAATCCT1004300.13394213275752576No Hit
GTGCGAGTCGTTAAATCACCGAGTGATTTAACTTAAATCAAAAATTTCGA995360.13274981704822347No Hit
GTGGGATTCTGACTTAGAGGCGTTCAGTCATAATCCCTCAGATGGTAGCT978670.13052389432023073No Hit
CGTTAACAGGTTTAAATTGTACCCATTCCGATAACCGGCCTCAAAGAGAA976070.13017713583654106No Hit
GTCAAACTCCCCGCCTGACACTGTCTTCGGTGCGAGTCGTTAAATCACCG966810.1289421421600154No Hit
CTAGACCTGTACGGGTGAAAAAAGTTACGGTAGGTTATTCCTTGGAGTCG963260.1284686834611314No Hit
GCCAGTTCTAAGTTGACTGTTTAACGCCGGCCGAAATATCAAATAAAACA952750.12706697897513958No Hit
CCCTTACCTACATTATTCTATCGACTAGAGGCTGTTCACCTTGGAGACCT950970.12682958278245968No Hit
GTCTGGTGCCAGCAGCCGCGGTAATTCCAGCTCTCAAAGTGTATATCGTC944200.12592667703839075No Hit
ATTGAGAGCTCTTTCATGATTCGGTGGTTGGTGGTGCATGGCCGTTCTTA937680.12505711345621504No Hit
CCCGTTACAACCATGGTAGGCACATAAACTACCATCGAAAGTTGATAAGG933530.12450363356878724No Hit
CTCTTCAAAGTTCTTTGCAACTTTCCCTCACGGTACTTGTTTGCTATCGG928740.12386479774691274No Hit
CCCTCTTGAACTCTCTCTTCAAAGTTCTTTGCAACTTTCCCTCACGGTAC916440.12222436338176532No Hit
GCCCCAGTCAAACTCCCCGCCTGACACTGTCTTCGGTGCGAGTCGTTAAA916310.12220702545758083No Hit
CTCTTGAACTCTCTCTTCAAAGTTCTTTGCAACTTTCCCTCACGGTACTT911120.12151484217667716No Hit
CGCAAATGAACTCGGAAACATTGAGGAAAGCCCGAGCCAGAGTTCTCTTT911000.12149883793896839No Hit
CCCAGTCAAACTCCCCGCCTGACACTGTCTTCGGTGCGAGTCGTTAAATC900360.12007979552879208No Hit
GTGAAATACCACTACTTTCATCGATTCTTTACTTATTCGGTAAAACGAGA896350.1195449872520245No Hit
GGCCGTTCTTAGTTGGTGGAGTGATTTGTCTGGTTTATTCCGATAACGAG884570.11797390458361502No Hit
CAACGGTTGAGTATAAGTCTCCCGCTCAAGCGCCATCCATTTTCAGGGCT880310.11740575414495419No Hit
GCCGTTCTTAGTTGGTGGAGTGATTTGTCTGGTTTATTCCGATAACGAGC878990.11722970753015789No Hit
GTCTGCAAGTGATCTTGCGCCCCGACATTGTACCAGAGTAATCCGGTTTT869160.11591869372451567No Hit
AGGTGCTAGACCTGTACGGGTGAAAAAAGTTACGGTAGGTTATTCCTTGG862650.11505046382881567No Hit
CTCGGATTTTCATGGATCGAACAAAGCGCACGAGACACCACAGGAACCGT861290.11486908246811647No Hit
CTGCTGCCTTCCTTGGATGTGGTAGCCGTTTCTCAGGCTCCCTCTCCGGA852390.11368210150471708No Hit
GTCAGATAGGTGATTAACGTTCACATCGAGATGTAACTTTATTGCATACA841890.112281730705201No Hit
CCCGCCTGACACTGTCTTCGGTGCGAGTCGTTAAATCACCGAGTGATTTA840620.11211235252278334No Hit
CCTCATTCTGAGAGTGGGTAATTTGCGCGCCTGCTGCCTTCCTTGGATGT838890.11188162476248212No Hit
GCCGCAGGCTCCACTCCTGGTGGTGCCCTTCCGTCAATTTCTTTAAGTTT838560.11183761310878304No Hit
CACGCCCTCTTGAACTCTCTCTTCAAAGTTCTTTGCAACTTTCCCTCACG828110.11044391074164557No Hit
GGCATTAGCTGTAATAGTATGAATATGTTCATAATTACATAATAGTGCCT827340.11034121688301439No Hit
CGTCTGCAAGTGATCTTGCGCCCCGACATTGTACCAGAGTAATCCGGTTT826540.11023452196495603No Hit
ATTTTATTTTAAAAAACGGCACTAGCCAATATCTTAGGATCGACTGACCC826290.1102011798030628No Hit
GCGCGGTAACGCAAATGAACTCGGAAACATTGAGGAAAGCCCGAGCCAGA823750.10986242343822747No Hit
CTAGCATATACGCAAGTTATTGGGTAGTAAACCTAATTTAAAAGATAAAA822270.10966503783981947No Hit
CTTCAAAGTTCTTTGCAACTTTCCCTCACGGTACTTGTTTGCTATCGGTC821380.10954633974347955No Hit
CCCCCGTCCGTCCCTCTTAACCATTATCTCAGATCAGAAAACCAACAAAA814740.10866077192359508No Hit
CCCACTTGGAGCACACATTCAATGTCTATGCTTCATAAAAAATTTAAGCA810250.10806194669599248No Hit
CCCACATTCAACTGCTGTTCACGTGGAACCCTTCTCCACTTCAGTCTTCA797680.10638550279600036No Hit
GCGCGTGCAGCCCTGGACATCTAAGGGCATCACAGACCTGTTATTGCTCA794520.1059640578696698No Hit
CCGTGTCCAGGTACGGGAATATTAACCCGTTTCCCTTTCGATTTATCAGA793460.10582268710324244No Hit
CTCCTCATTCTGAGAGTGGGTAATTTGCGCGCCTGCTGCCTTCCTTGGAT783550.10450100380579438No Hit
CCCGAGTCCGTGCCAGTTCTAAGTTGACTGTTTAACGCCGGCCGAAATAT779640.1039795323937841No Hit
GCGAGACTCTAGCCTACTAAATAGTTACTGGATAATTTTATCGTCCAGAC771880.10294459168861793No Hit
GTGATTTGTCTGGTTTATTCCGATAACGAGCGAGACTCTAGCCTACTAAA771770.1029299211373849No Hit
GTTGCGATAGTAATCCTGCTCAGTACGAGAGGAACCGCAGGTTCAGACAT765700.10212037344661702No Hit
GCCGTTTCTCAGGCTCCCTCTCCGGAGTCGAACCCTTATTCTCCGTTACC765020.10202968276626741No Hit
GTCCGTCCCTCTTAACCATTATCTCAGATCAGAAAACCAACAAAATAGAA754380.10061064035609109No Hit
GCGATAGTAATCCTGCTCAGTACGAGAGGAACCGCAGGTTCAGACATTTG753810.10053462022697451No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCCTTGT1386650.084.380391
GTTAGTT1498750.082.9254461
TAGTTTT1575200.078.919153
TTAGTTT1594150.077.953642
CCTGGCC434750.076.679675
CCTTGTC1578400.074.3422242
ACCTGGC457700.072.8590854
AGTTTTA1730900.072.240364
CACCTGG482950.069.190363
CCGACAA152750.068.95118145
CCAGACG168450.067.950696
GAGTCTA144100.065.478562
GTAGCGC884750.064.8227548
GCCCTCT1653800.064.718121
AGACAGG241500.063.5489771
CCAGTGT901950.062.9599953
TCCAGTG900900.062.663262
CTCTTGA1661700.062.146074
TGGCCCG538500.061.804577
CTGGCCC539600.061.703156
Produced by FastQC (version 0.11.2)