FastQCFastQC Report
Wed 14 Dec 2016
HVMVCBGXY_n02_brugia3-l3d6.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHVMVCBGXY_n02_brugia3-l3d6.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences78954245
Sequences flagged as poor quality0
Sequence length151
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCCTCTTGAACTCTCTCTTCAAAGTTCTTTGCAACTTTCCCTCACGGTA7284400.9226103042338002No Hit
GCCTTGTCTAGGTGGGATTCTGACTTAGAGGCGTTCAGTCATAATCCCTC5714750.7238052874801095No Hit
CCCGTCCGTCCCTCTTAACCATTATCTCAGATCAGAAAACCAACAAAATA5337040.6759661877584923No Hit
GTTAGTTTTACCCTACTGTGATATGTTGTTGCGATAGTAATCCTGCTCAG4737110.5999816729296823No Hit
GAAAAAAGTTACGGTAGGTTATTCCTTGGAGTCGGGTTGTTTGAGATTGC4698050.5950345038446001No Hit
CTCCAGTGTAGCGCGCGTGCAGCCCTGGACATCTAAGGGCATCACAGACC4557090.5771811256000231No Hit
AAAAAAGTTACGGTAGGTTATTCCTTGGAGTCGGGTTGTTTGAGATTGCA4141370.5245278452095894No Hit
GCACCTTCTATGGACTGTAGCCCCGCTCAAGGGGACTTTGGGTGTAAAAA4126200.5226064792336371No Hit
AAAGAAACTAACGAGGATTTCCCTAGTAACGGCGAGTGAAAAGGAAAAAG3707460.4695706988268965No Hit
GTTTTACCCTACTGTGATATGTTGTTGCGATAGTAATCCTGCTCAGTACG3465000.43886177367664025No Hit
CTCGCTCGTTATCGGAATAAACCAGACAAATCACTCCACCAACTAAGAAC3384410.4286545960891653No Hit
CTTGTCTAGGTGGGATTCTGACTTAGAGGCGTTCAGTCATAATCCCTCAG3275750.4148921948401888No Hit
CGCGCGTGCAGCCCTGGACATCTAAGGGCATCACAGACCTGTTATTGCTC3036210.3845531041427855No Hit
CGCGCCTGCTGCCTTCCTTGGATGTGGTAGCCGTTTCTCAGGCTCCCTCT2971960.3764154796236732No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG2916510.36939242468850153No Hit
AAAAAGTTACGGTAGGTTATTCCTTGGAGTCGGGTTGTTTGAGATTGCAG2856230.36175762303850795No Hit
GCGCCTGCTGCCTTCCTTGGATGTGGTAGCCGTTTCTCAGGCTCCCTCTC2773410.3512680033860117No Hit
CTCTCTTCAAAGTTCTTTGCAACTTTCCCTCACGGTACTTGTTTGCTATC2609040.3304496167368835No Hit
CTCTTAACCATTATCTCAGATCAGAAAACCAACAAAATAGAACCGAGATC2552590.32329990616717313No Hit
ATTAAAACCAATCGGATTATTAATTTTAATCCGTTAATTGGTGACTCTGA2523840.31965855667418513No Hit
CTCGTTAACAGGTTTAAATTGTACCCATTCCGATAACCGGCCTCAAAGAG2364920.29953044323329286No Hit
CTCACCTGGCCCGGACACCGTGAGGATTGACAGATTGAGAGCTCTTTCAT2363020.2992897975276694No Hit
CGCCCTCTTGAACTCTCTCTTCAAAGTTCTTTGCAACTTTCCCTCACGGT2329560.295051899996004No Hit
GCGGATATGGGTACGATCTGGCACGAAATTCAAATAGCTTCCCTCGGATT2295220.2907025455059446No Hit
CTCCGTGTCCAGGTACGGGAATATTAACCCGTTTCCCTTTCGATTTATCA2186030.27687301677066256No Hit
CTCTTCAAAGTTCTTTGCAACTTTCCCTCACGGTACTTGTTTGCTATCGG2163400.27400679976105147No Hit
CTTGAACTCTCTCTTCAAAGTTCTTTGCAACTTTCCCTCACGGTACTTGT2125010.2691444899511103No Hit
ATTAGATTAAAACCAATCGGATTATTAATTTTAATCCGTTAATTGGTGAC2030020.257113471226278No Hit
CCCGCATTGAGCACTCTAATTTTTTCAAGGTAAACTTGCTAGCCACCATA2026800.2567056400830633No Hit
CTGAATAGCTATGGCTGATCGCATGGTCTTGTACCGGCGACGTATCTATC2006650.2541535290471082No Hit
CTTCTATGGACTGTAGCCCCGCTCAAGGGGACTTTGGGTGTAAAAATATG1985240.2514418319116344No Hit
CGGAAACCTCTCGTAGAGTAAAAGGGCAAAAGCTCGCTTGATCTTGATTT1956990.24786381023591575No Hit
CTCTGAATAGCTATGGCTGATCGCATGGTCTTGTACCGGCGACGTATCTA1938860.2455675435817289No Hit
GCTAGACCTGTACGGGTGAAAAAAGTTACGGTAGGTTATTCCTTGGAGTC1911180.24206171561769732No Hit
CTCCACTCCTGGTGGTGCCCTTCCGTCAATTTCTTTAAGTTTCAGCTTTG1860470.23563900839023919No Hit
CTGCAAGTGATCTTGCGCCCCGACATTGTACCAGAGTAATCCGGTTTTGG1766540.22374224463801787No Hit
CTCCACTTCAGTCTTCAAGGATCTCACTTGAATATTTGCTACTACCACCA1728270.21889513350422132No Hit
GCTACTACCACCAAGATCTGCACCAATGGAAGCTTCAACCGGGCCTACGC1708140.21634555558095706No Hit
GTGGCTAGCAAGTTTACCTTGAAAAAATTAGAGTGCTCAATGCGGGCTAA1652850.2093427655473116No Hit
CAAACGTTGAGTTAAAGTGCCAAACTTTCCGCTCATGAGACCCCATGAAA1611800.20414355174949742No Hit
CTTCAAAGTTCTTTGCAACTTTCCCTCACGGTACTTGTTTGCTATCGGTC1608620.20374078683166436No Hit
CCCTGTGGTAACTTTTCTGACACCTCTTGCTAGAAACCTTCTAAAAAATT1581020.20024509131839588No Hit
AAGAAACTAACGAGGATTTCCCTAGTAACGGCGAGTGAAAAGGAAAAAGC1563610.19804001672107688No Hit
GCGCGCCTGCTGCCTTCCTTGGATGTGGTAGCCGTTTCTCAGGCTCCCTC1540480.19511047189419645No Hit
CCTCGTTAACAGGTTTAAATTGTACCCATTCCGATAACCGGCCTCAAAGA1503810.19046600977566183No Hit
GTCCCTCTTAACCATTATCTCAGATCAGAAAACCAACAAAATAGAACCGA1501970.1902329634081106No Hit
GTTACGGTAGGTTATTCCTTGGAGTCGGGTTGTTTGAGATTGCAGCCCAA1493440.18915259084549538No Hit
GTGACTCTGAATAGCTATGGCTGATCGCATGGTCTTGTACCGGCGACGTA1471450.18636743344198908No Hit
GCGGTGTGTACAAAGGGCAGGGACGTAATCAACGTGAGCTGATGACTCAC1414110.1791049993575393No Hit
TCTCTTCAAAGTTCTTTGCAACTTTCCCTCACGGTACTTGTTTGCTATCG1395890.17679733369624395No Hit
CCCCAGTCAAACTCCCCGCCTGACACTGTCTTCGGTGCGAGTCGTTAAAT1393810.17653388997640343No Hit
GACAGATTGAGAGCTCTTTCATGATTCGGTGGTTGGTGGTGCATGGCCGT1386950.17566503232346786No Hit
GTACGGGTGAAAAAAGTTACGGTAGGTTATTCCTTGGAGTCGGGTTGTTT1332390.16875470090303568No Hit
CCCCGTCCGTCCCTCTTAACCATTATCTCAGATCAGAAAACCAACAAAAT1320200.16721076871800877No Hit
CCCACTTGGAGCACACATTCAATGTCTATGCTTCATAAAAAATTTAAGCA1316900.16679280512403102No Hit
GTCCGTCCCTCTTAACCATTATCTCAGATCAGAAAACCAACAAAATAGAA1302400.16495629842321968No Hit
CCCGTTACAACCATGGTAGGCACATAAACTACCATCGAAAGTTGATAAGG1290150.16340476690012046No Hit
CAGGGACGTAATCAACGTGAGCTGATGACTCACACTTACTAGGAATTCCT1289180.1632819109346179No Hit
CGCCTGCTGCCTTCCTTGGATGTGGTAGCCGTTTCTCAGGCTCCCTCTCC1256370.15912633956540273No Hit
CCCCCGTCCGTCCCTCTTAACCATTATCTCAGATCAGAAAACCAACAAAA1255650.15903514750853484No Hit
CACGCCCTCTTGAACTCTCTCTTCAAAGTTCTTTGCAACTTTCCCTCACG1234300.15633104971113332No Hit
GCGAGACTCTAGCCTACTAAATAGTTACTGGATAATTTTATCGTCCAGAC1229600.15573576822880136No Hit
GTGAGGATTGACAGATTGAGAGCTCTTTCATGATTCGGTGGTTGGTGGTG1219090.1544046175097995No Hit
GTCTAGGTGGGATTCTGACTTAGAGGCGTTCAGTCATAATCCCTCAGATG1207100.15288601645168035No Hit
CTCCGTTACCCGTTACAACCATGGTAGGCACATAAACTACCATCGAAAGT1205590.1526947664435269No Hit
GTCAGATAGGTGATTAACGTTCACATCGAGATGTAACTTTATTGCATACA1174080.14870384739921202No Hit
GCGAGTCGTTAAATCACCGAGTGATTTAACTTAAATCAAAAATTTCGAAA1154690.1462479946455064No Hit
GCCAGTTTTCCCATACGTTACCTTAATTGGTTGCATATGGTGGCTAGCAA1149870.1456375144870298No Hit
GCGCGTGCAGCCCTGGACATCTAAGGGCATCACAGACCTGTTATTGCTCA1144180.1449168439265045No Hit
AAAAGTTACGGTAGGTTATTCCTTGGAGTCGGGTTGTTTGAGATTGCAGC1132820.14347803591814476No Hit
CCCTCTTGAACTCTCTCTTCAAAGTTCTTTGCAACTTTCCCTCACGGTAC1107500.14027111525162453No Hit
GCCCACTAGGCACTCGCATTCCACGCCCGGCTCCACGCCAGCGAGCCGGG1102110.13958844138146087No Hit
GTCTGGTGCCAGCAGCCGCGGTAATTCCAGCTCTCAAAGTGTATATCGTC1101580.13952131389515535No Hit
CCTGTGGTAACTTTTCTGACACCTCTTGCTAGAAACCTTCTAAAAAATTC1071990.13577357366915482No Hit
CTGAAGTTTCCCTCAGGATAGCTGGAGTACGTTAAATAAAGCAGTTATAT1070600.13559752233714603No Hit
GCCGTTCTTAGTTGGTGGAGTGATTTGTCTGGTTTATTCCGATAACGAGC1052540.13331012157737687No Hit
CTCTTGAACTCTCTCTTCAAAGTTCTTTGCAACTTTCCCTCACGGTACTT999990.1266543679823675No Hit
GTCGTATTTAGCCTTAGATGAGGTTTACCACCCTCTTTGGGCTGCAATCT998980.12652644579148342No Hit
CTCAGGACCTGGTCCATCCATTGGATGAGAACTAGGCTCCTAGGCTAATA997960.12639725704425392No Hit
CTTTGGGCTGCAATCTCAAACAACCCGACTCCAAGGAATAACCTACCGTA977750.12383754667022653No Hit
GGCCGTTCTTAGTTGGTGGAGTGATTTGTCTGGTTTATTCCGATAACGAG956430.12113724854186118No Hit
CGAGTATTCAGGCATTAGCCCGCATTGAGCACTCTAATTTTTTCAAGGTA953850.1208104770047513No Hit
CCTTATTCTCCGTTACCCGTTACAACCATGGTAGGCACATAAACTACCAT941320.11922348190398123No Hit
GCCGTTTCTCAGGCTCCCTCTCCGGAGTCGAACCCTTATTCTCCGTTACC937310.11871559280948099No Hit
CTAAGAAGTTGTCTGGACGATAAAATTATCCAGTAACTATTTAGTAGGCT937180.11869912757699096No Hit
CTCAACCAGACGTACCATCGGGAATCAACCCAATGGTGCAATGTGCATTC934160.11831662756068403No Hit
GGAAATGTAGCGTATAGGTATAACTATCCGTAACATATTTTTACACCCAA927000.11740977321738687No Hit
CCTCATTCTGAGAGTGGGTAATTTGCGCGCCTGCTGCCTTCCTTGGATGT926320.11732364738590052No Hit
CGTCCCTCTTAACCATTATCTCAGATCAGAAAACCAACAAAATAGAACCG911940.11550233936123383No Hit
ATTGAGAGCTCTTTCATGATTCGGTGGTTGGTGGTGCATGGCCGTTCTTA910560.11532755458557042No Hit
CTAGACCTGTACGGGTGAAAAAAGTTACGGTAGGTTATTCCTTGGAGTCG902100.1142560479173729No Hit
CTGGTGCCAGCAGCCGCGGTAATTCCAGCTCTCAAAGTGTATATCGTCAT901670.11420158599452127No Hit
AGGTGCTAGACCTGTACGGGTGAAAAAAGTTACGGTAGGTTATTCCTTGG898770.11383428465435899No Hit
GTGATTTGTCTGGTTTATTCCGATAACGAGCGAGACTCTAGCCTACTAAA893860.11321240548877391No Hit
GGAAGTTGGAAACCGCTAAGGAGTGTGTAACAACTCACCTGCCAAATCAA887740.11243727300539699No Hit
TGAGGATTGACAGATTGAGAGCTCTTTCATGATTCGGTGGTTGGTGGTGC884310.1120028441789292No Hit
GCCGCAGGCTCCACTCCTGGTGGTGCCCTTCCGTCAATTTCTTTAAGTTT878490.1112657083859139No Hit
CTTGATTAATGAAGACATTCTTGGCAAATGCTTTCGCAGTCGTACGTCTC854820.10826776951638256No Hit
CCCACATTCAACTGCTGTTCACGTGGAACCCTTCTCCACTTCAGTCTTCA852550.10798026122597969No Hit
GGCCCACTAGGCACTCGCATTCCACGCCCGGCTCCACGCCAGCGAGCCGG851150.1078029433376255No Hit
GCCCGTTACAACCATGGTAGGCACATAAACTACCATCGAAAGTTGATAAG849220.10755849796296577No Hit
GAGGATTGACAGATTGAGAGCTCTTTCATGATTCGGTGGTTGGTGGTGCA848950.10752430094164032No Hit
GGAAACCTCTCGTAGAGTAAAAGGGCAAAAGCTCGCTTGATCTTGATTTC846960.10727225622890828No Hit
CAGATTGAGAGCTCTTTCATGATTCGGTGGTTGGTGGTGCATGGCCGTTC835600.10583344822054849No Hit
GACAGGTTAGTTTTACCCTACTGTGATATGTTGTTGCGATAGTAATCCTG831140.10526856409050585No Hit
CGTTAACAGGTTTAAATTGTACCCATTCCGATAACCGGCCTCAAAGAGAA828690.10495825778588598No Hit
CACGAGTATTCAGGCATTAGCCCGCATTGAGCACTCTAATTTTTTCAAGG824480.104425037564478No Hit
CTCCTCATTCTGAGAGTGGGTAATTTGCGCGCCTGCTGCCTTCCTTGGAT821100.10399694151973717No Hit
GGCTGATCGCATGGTCTTGTACCGGCGACGTATCTATCAAGTGTCTGCCT818590.10367903587704498No Hit
CTCCTACTCGTCGCGGCGTAGCGTCCGCGGGGGCCCGACGCCGCGGGGGG805240.10198818315595318No Hit
CTGGCCCGGACACCGTGAGGATTGACAGATTGAGAGCTCTTTCATGATTC804950.10195145302193695No Hit
CTTAACCATTATCTCAGATCAGAAAACCAACAAAATAGAACCGAGATCCT802230.10160694969599164No Hit
CTCCGGAGTCGAACCCTTATTCTCCGTTACCCGTTACAACCATGGTAGGC800590.10139923445534817No Hit
GGAAAAAGCCCAACGCTGAATCCAATAGACATTATATGGCTATATGGAAA799300.10123584868679321No Hit
AAACTAACGAGGATTTCCCTAGTAACGGCGAGTGAAAAGGAAAAAGCCCA795290.10072795959229297No Hit
CTCTTGCTAGAAACCTTCTAAAAAATTCAAAAGGATCGATAGGCCACGCT794320.10060510362679043No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCCTTGT955350.092.080371
CCTTGTC1133600.077.8360442
CCTGGCC499650.073.175825
CCAGACG202250.071.755956
TCCAGTG1021550.070.419732
TAGTTTT1059400.070.0048753
GTTAGTT1067350.069.350761
CCAGTGT1055000.068.5796053
GTAGCGC1079300.068.010078
ACCTGGC551300.066.811874
CACCTGG557200.066.198713
TTAGTTT1144300.064.771922
AGAAACT973300.064.50073
TTGTCTA1390500.063.5962334
GCCCTCT1928300.062.4243621
CTCTTGA1867800.062.0165634
TGTCTAG1433050.061.8619425
GAAACTA1019300.061.6131634
ACTAACG936650.060.919637
CTCCAGT1192000.060.8397181
Produced by FastQC (version 0.11.2)