FastQCFastQC Report
Wed 14 Dec 2016
HVMVCBGXY_n02_brugia2-l3d9.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHVMVCBGXY_n02_brugia2-l3d9.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences73142895
Sequences flagged as poor quality0
Sequence length151
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCCTCTTGAACTCTCTCTTCAAAGTTCTTTGCAACTTTCCCTCACGGTA8500991.162244125010365No Hit
GCCTTGTCTAGGTGGGATTCTGACTTAGAGGCGTTCAGTCATAATCCCTC4551870.6223256544603546No Hit
GTTAGTTTTACCCTACTGTGATATGTTGTTGCGATAGTAATCCTGCTCAG4526050.6187955781624449No Hit
GAAAAAAGTTACGGTAGGTTATTCCTTGGAGTCGGGTTGTTTGAGATTGC4031840.5512278396965282No Hit
AAAAAAGTTACGGTAGGTTATTCCTTGGAGTCGGGTTGTTTGAGATTGCA3407180.4658251495240925No Hit
GTTTTACCCTACTGTGATATGTTGTTGCGATAGTAATCCTGCTCAGTACG3386520.4630005416110478No Hit
CTCTCTTCAAAGTTCTTTGCAACTTTCCCTCACGGTACTTGTTTGCTATC3368730.46056831630741435No Hit
CTTGTCTAGGTGGGATTCTGACTTAGAGGCGTTCAGTCATAATCCCTCAG3167670.4330796586599423No Hit
CTCGCTCGTTATCGGAATAAACCAGACAAATCACTCCACCAACTAAGAAC2790730.3815449197081959No Hit
CTCCTACTCGTCGCGGCGTAGCGTCCGCGGGGGCCCGACGCCGCGGGGGG2714780.37116113602011513No Hit
AAAAAGTTACGGTAGGTTATTCCTTGGAGTCGGGTTGTTTGAGATTGCAG2663570.36415977245636777No Hit
GCTAGACCTGTACGGGTGAAAAAAGTTACGGTAGGTTATTCCTTGGAGTC2446990.3345492409071312No Hit
CTTGAACTCTCTCTTCAAAGTTCTTTGCAACTTTCCCTCACGGTACTTGT2438380.3333720930788971No Hit
CGCCCTCTTGAACTCTCTCTTCAAAGTTCTTTGCAACTTTCCCTCACGGT2407380.32913381402253766No Hit
CTGAATAGCTATGGCTGATCGCATGGTCTTGTACCGGCGACGTATCTATC2252110.3079055046973462No Hit
CTCCGTGTCCAGGTACGGGAATATTAACCCGTTTCCCTTTCGATTTATCA2180450.298108244143194No Hit
GCGCCTGCTGCCTTCCTTGGATGTGGTAGCCGTTTCTCAGGCTCCCTCTC2148120.2936881292434487No Hit
CTCTGAATAGCTATGGCTGATCGCATGGTCTTGTACCGGCGACGTATCTA2141580.2927939890812361No Hit
CGCGCCTGCTGCCTTCCTTGGATGTGGTAGCCGTTTCTCAGGCTCCCTCT2073050.28342465799309696No Hit
GCGGATATGGGTACGATCTGGCACGAAATTCAAATAGCTTCCCTCGGATT2033410.2780051295481263No Hit
GTCGGAATCCGCTAAGGAGTGTGTAACAACTCACCTGCCGAATCAACTAG1985810.27149732041642594No Hit
CTCCAGTGTAGCGCGCGTGCAGCCCTGGACATCTAAGGGCATCACAGACC1961870.26822427523548253No Hit
GTACGGGTGAAAAAAGTTACGGTAGGTTATTCCTTGGAGTCGGGTTGTTT1832080.2504795578572601No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG1743920.23842643909569072No Hit
GTTACGGTAGGTTATTCCTTGGAGTCGGGTTGTTTGAGATTGCAGCCCAA1725180.23586433104678178No Hit
CTCTTCAAAGTTCTTTGCAACTTTCCCTCACGGTACTTGTTTGCTATCGG1606760.2196741050514886No Hit
CTCCACTTCAGTCTTCAAGGATCTCACTTGAATATTTGCTACTACCACCA1596300.21824402766666534No Hit
CTCGTTAACAGGTTTAAATTGTACCCATTCCGATAACCGGCCTCAAAGAG1578570.21582000548378624No Hit
GTTTTATCCGGTAAAGCGAATGATTAGAGGTCTTGGGGCCGAAACGATCT1564000.21382801432729726No Hit
CTTCAAAGTTCTTTGCAACTTTCCCTCACGGTACTTGTTTGCTATCGGTC1433920.196043648532096No Hit
GTGGCGTTCAGCCACCCGAGATTGAGCAATAACAGGTCTGTGATGCCCTT1413100.19319716563037326No Hit
CTCACCTGGCCCGGACACCGTGAGGATTGACAGATTGAGAGCTCTTTCAT1404220.19198310375874514No Hit
CAAACGTTGAGTTAAAGTGCCAAACTTTCCGCTCATGAGACCCCATGAAA1395240.19075537001919324No Hit
GCGGTGTGTACAAAGGGCAGGGACTTAATCAACGCAAGCTTATGACCCGC1353570.1850583026553707No Hit
GCCCACTAGGCACTCGCATTCCACGCCCGGCTCCACGCCAGCGAGCCGGG1351210.18473564657237043No Hit
CCCGTTCCGACTGCCGGCGACGGCCGGGTATGGGCCCGACGCTCCAGCGC1329400.1817538121782027No Hit
GTCTAGGTGGGATTCTGACTTAGAGGCGTTCAGTCATAATCCCTCAGATG1318080.1802061567292353No Hit
CTCTTGAACTCTCTCTTCAAAGTTCTTTGCAACTTTCCCTCACGGTACTT1312130.17939268058777275No Hit
CTTGATTAATGAAGACATTCTTGGCAAATGCTTTCGCAGTCGTACGTCTC1280100.17501358129179873No Hit
GTGACTCTGAATAGCTATGGCTGATCGCATGGTCTTGTACCGGCGACGTA1279400.17491787821633256No Hit
CGCCTGCTGCCTTCCTTGGATGTGGTAGCCGTTTCTCAGGCTCCCTCTCC1266730.17318565255039467No Hit
GGCGTTCAGCCACCCGAGATTGAGCAATAACAGGTCTGTGATGCCCTTAG1247740.1705893648316764No Hit
GGAAGTCGGAATCCGCTAAGGAGTGTGTAACAACTCACCTGCCGAATCAA1243240.1699741307751081No Hit
CCCTCTTGAACTCTCTCTTCAAAGTTCTTTGCAACTTTCCCTCACGGTAC1216980.1663838982583339No Hit
CTAGACCTGTACGGGTGAAAAAAGTTACGGTAGGTTATTCCTTGGAGTCG1214900.16609952340552012No Hit
CGGAAACCTCTCGTAGAGTAAAAGGGCAAAAGCTCGCTTGATCTTGATTT1203810.16458331325277734No Hit
AGGTGCTAGACCTGTACGGGTGAAAAAAGTTACGGTAGGTTATTCCTTGG1179050.16119815875485924No Hit
TCTCTTCAAAGTTCTTTGCAACTTTCCCTCACGGTACTTGTTTGCTATCG1129930.15448253723071803No Hit
AAAAGTTACGGTAGGTTATTCCTTGGAGTCGGGTTGTTTGAGATTGCAGC1052350.14387590209548037No Hit
CGCGCGTGCAGCCCTGGACATCTAAGGGCATCACAGACCTGTTATTGCTC1052080.14383898805208625No Hit
GGCTGATCGCATGGTCTTGTACCGGCGACGTATCTATCAAGTGTCTGCCT1048110.14329621489551378No Hit
GTGAAATACCACTACTTTCATCGATTCTTTACTTATTCGGTAAAACGAGA1045400.1429257072747804No Hit
CACGCCCTCTTGAACTCTCTCTTCAAAGTTCTTTGCAACTTTCCCTCACG1041040.14232961383330534No Hit
CTCCACTCCTGGTGGTGCCCTTCCGTCAATTTCTTTAAGTTTCAGCTTTG1021900.13971281831270144No Hit
ATTAAAACCAATCGGATTATTAATTTTAATCCGTTAATTGGTGACTCTGA981900.13424407114320538No Hit
CTTGGAGTCGGGTTGTTTGAGATTGCAGCCCAAAGAGGGTGGTAAACCTC969620.13256516576217006No Hit
GCTACTACCACCAAGATCTGCACCAATGGAAGCTTCAACCGGGCCTACGC963500.13172844744523715No Hit
CTGGGGTCTGATGAGCGTCGGCATCGGGCGCCTTAACCCGGCGTTCGGTT948480.12967493288309137No Hit
CCCGTCCGTCCCTCTTAACCATTATCTCAGATCAGAAAACCAACAAAATA947310.12951497202838363No Hit
CTGCAAGTGATCTTGCGCCCCGACATTGTACCAGAGTAATCCGGTTTTGG942610.12887239423596783No Hit
GACAGATTGAGAGCTCTTTCATGATTCGGTGGTTGGTGGTGCATGGCCGT926860.12671907503797875No Hit
GGCCCACTAGGCACTCGCATTCCACGCCCGGCTCCACGCCAGCGAGCCGG921380.12596985667575777No Hit
GTTAGTTTTACCCTACTGATGATGTGTTGTTGCCATGGTAATCCTGCTCA910310.12445638089659972No Hit
GCGCGCCTGCTGCCTTCCTTGGATGTGGTAGCCGTTTCTCAGGCTCCCTC908920.12426634193245974No Hit
CAGGGACGTAATCAACGTGAGCTGATGACTCACACTTACTAGGAATTCCT894010.12222786642503006No Hit
CCTCGTTAACAGGTTTAAATTGTACCCATTCCGATAACCGGCCTCAAAGA887310.12131185127413947No Hit
CGCAAATGAACTCGGAAACATTGAGGAAAGCCCGAGCCAGAGTTCTCTTT882780.12069251565719405No Hit
CCCGTTACAACCATGGTAGGCACATAAACTACCATCGAAAGTTGATAAGG854610.11684115046307642No Hit
CTCCGTTACCCGTTACAACCATGGTAGGCACATAAACTACCATCGAAAGT850940.11633939291027515No Hit
GTGAGGATTGACAGATTGAGAGCTCTTTCATGATTCGGTGGTTGGTGGTG844680.11548353397824902No Hit
CCTTATTCTCCGTTACCCGTTACAACCATGGTAGGCACATAAACTACCAT830570.11355443341420927No Hit
GTACAAAGGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACT825810.11290365250103923No Hit
AAAGAAACTAACCAGGATTCCCTCAGTAACGGCGAGTGAACAGGGAAGAG819890.11209427791995383No Hit
GGAATAATGAAATAGGATCTCGGTTCTATTTTGTTGGTTTTCTGATCTGA818750.11193841862562318No Hit
CCCACATTCAACTGCTGTTCACGTGGAACCCTTCTCCACTTCAGTCTTCA818660.11192611394449181No Hit
CCCCAGTCAAACTCCCCGCCTGACACTGTCTTCGGTGCGAGTCGTTAAAT815290.11146537199546176No Hit
CTCTTAACCATTATCTCAGATCAGAAAACCAACAAAATAGAACCGAGATC809620.11069017708418569No Hit
ATTAGATTAAAACCAATCGGATTATTAATTTTAATCCGTTAATTGGTGAC806490.11026224761817262No Hit
GTGGGATTCTGACTTAGAGGCGTTCAGTCATAATCCCTCAGATGGTAGCT798710.10919857629370563No Hit
CCCTGTGGTAACTTTTCTGACACCTCTTGCTAGAAACCTTCTAAAAAATT798000.10910150603144707No Hit
GTACGATCTGGCACGAAATTCAAATAGCTTCCCTCGGATTTTCATGGATC796930.10895521704466304No Hit
CCTCCTACTCGTCGCGGCGTAGCGTCCGCGGGGGCCCGACGCCGCGGGGG795860.10880892805787902No Hit
GCACCTTCTATGGACTGTAGCCCCGCTCAAGGGGACTTTGGGTGTAAAAA788770.10783959262208585No Hit
ATTTTATTTTAAAAAACGGCACTAGCCAATATCTTAGGATCGACTGACCC786660.10755111620889493No Hit
GTGCTAGACCTGTACGGGTGAAAAAAGTTACGGTAGGTTATTCCTTGGAG782150.10693451496553426No Hit
CTCGGATTTTCATGGATCGAACAAAGCGCACGAGACACCACAGGAACCGT776460.10615658568067343No Hit
GGAAGTTGGAAACCGCTAAGGAGTGTGTAACAACTCACCTGCCAAATCAA775260.10599252326558854No Hit
CTTGGATGTGGTAGCCGTTTCTCAGGCTCCCTCTCCGGAGTCGAACCCTT767820.10497533629206227No Hit
GGTAAAGCGAATGATTAGAGGTCTTGGGGCCGAAACGATCTCAACCTATT746840.10210697840166157No Hit
TAGACCTGTACGGGTGAAAAAAGTTACGGTAGGTTATTCCTTGGAGTCGG744860.10183627541677151No Hit
CTTGATTAATGAAAACATTCTTGGCAAATGCTTTCGCTCTGGTCCGTCTT744600.10180072856016979No Hit
CTCGTTCATGGGGAATAATTGCAATCCCCGATCCCCATCACGAATGGGGT737680.10085463529984695No Hit
CTGCTGCCTTCCTTGGATGTGGTAGCCGTTTCTCAGGCTCCCTCTCCGGA736970.10075756503758841No Hit
CTACCACCAAGATCTGCACCAATGGAAGCTTCAACCGGGCCTACGCCCAA735750.10059076824891877No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCCTTGT826850.084.156381
TAGTTTT1109950.074.4226763
CCTGGCC311600.072.4617545
AGACAGG190150.067.897751
AGAACCC192450.067.45688145
CCTTGTC1038950.067.216942
CCAGACG149400.066.7639856
GTTAGTT1250200.066.2351461
GCCCTCT2186700.065.082341
TTAGTTT1321350.062.5967142
TGGCGTT357700.062.2956432
CTCTTGA2260650.062.069014
GTGTCCA588300.058.783965
TCTTGAA2400050.058.684465
TGGCCCG384750.058.2952737
CGGTGTG526400.058.2312362
ATAACCA390550.057.052647145
CTGGCCC397350.056.7363746
CACCTGG400850.056.657713
TCCGTGT615300.056.330682
Produced by FastQC (version 0.11.2)