FastQCFastQC Report
Wed 14 Dec 2016
HVMVCBGXY_n02_brugia1-l3d9.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHVMVCBGXY_n02_brugia1-l3d9.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences92584749
Sequences flagged as poor quality0
Sequence length151
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTTAGTTTTACCCTACTGTGATATGTTGTTGCGATAGTAATCCTGCTCAG7179970.7755024534332323No Hit
GCCTTGTCTAGGTGGGATTCTGACTTAGAGGCGTTCAGTCATAATCCCTC6406710.6919832984587991No Hit
GCCCTCTTGAACTCTCTCTTCAAAGTTCTTTGCAACTTTCCCTCACGGTA3904670.42174008594007206No Hit
GTTTTACCCTACTGTGATATGTTGTTGCGATAGTAATCCTGCTCAGTACG3796220.41002649367230015No Hit
CTCACCTGGCCCGGACACCGTGAGGATTGACAGATTGAGAGCTCTTTCAT3304810.35694971749612886No Hit
GAAAAAAGTTACGGTAGGTTATTCCTTGGAGTCGGGTTGTTTGAGATTGC2599790.28080110688640525No Hit
CTCCAGTGTAGCGCGCGTGCAGCCCTGGACATCTAAGGGCATCACAGACC2362300.2551500139618027No Hit
CTTGTCTAGGTGGGATTCTGACTTAGAGGCGTTCAGTCATAATCCCTCAG2336910.25240766165494494No Hit
CTCGTTAACAGGTTTAAATTGTACCCATTCCGATAACCGGCCTCAAAGAG2309520.2494492910490042No Hit
AAAAAAGTTACGGTAGGTTATTCCTTGGAGTCGGGTTGTTTGAGATTGCA2273470.24555556120803437No Hit
GTACGATCTGGCACGAAATTCAAATAGCTTCCCTCGGATTTTCATGGATC2270970.24528553833418074No Hit
CTGCAAGTGATCTTGCGCCCCGACATTGTACCAGAGTAATCCGGTTTTGG2212800.23900264610535368No Hit
CAAACGTTGAGTTAAAGTGCCAAACTTTCCGCTCATGAGACCCCATGAAA2177600.2352007240414941No Hit
CTTGATTAATGAAGACATTCTTGGCAAATGCTTTCGCAGTCGTACGTCTC2168000.23416383620589606No Hit
CTCCGTTACCCGTTACAACCATGGTAGGCACATAAACTACCATCGAAAGT2145910.2317779140925251No Hit
GTCAGATAGGTGATTAACGTTCACATCGAGATGTAACTTTATTGCATACA2058280.22231307231820652No Hit
CCGACATCGAAGGATCAAAAAGCAACGTCGCTATGAACGCTTGGCTGCCA2039590.22029438131327658No Hit
GCGCCTGCTGCCTTCCTTGGATGTGGTAGCCGTTTCTCAGGCTCCCTCTC2024340.21864724178276923No Hit
CTCTCTTCAAAGTTCTTTGCAACTTTCCCTCACGGTACTTGTTTGCTATC1972220.21301780490866803No Hit
CTCCGTGTCCAGGTACGGGAATATTAACCCGTTTCCCTTTCGATTTATCA1964440.21217749372523545No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG1793520.19371656988560826No Hit
CCTTATTCTCCGTTACCCGTTACAACCATGGTAGGCACATAAACTACCAT1785480.1928481763232949No Hit
CGCGCCTGCTGCCTTCCTTGGATGTGGTAGCCGTTTCTCAGGCTCCCTCT1768560.1910206615130533No Hit
GTGAAATACCACTACTTTCATCGATTCTTTACTTATTCGGTAAAACGAGA1713540.1850779981052819No Hit
CTCGCTCGTTATCGGAATAAACCAGACAAATCACTCCACCAACTAAGAAC1680100.1814661721446153No Hit
ATTAAAACCAATCGGATTATTAATTTTAATCCGTTAATTGGTGACTCTGA1514960.16362954118933778No Hit
GTCTGGTGCCAGCAGCCGCGGTAATTCCAGCTCTCAAAGTGTATATCGTC1482920.16016892803802923No Hit
GACAGGTTAGTTTTACCCTACTGTGATATGTTGTTGCGATAGTAATCCTG1460030.1576965986050251No Hit
GGCATTAGCTGTAATAGTATGAATATGTTCATAATTACATAATAGTGCCT1454410.1570895871846021No Hit
GCTACTACCACCAAGATCTGCACCAATGGAAGCTTCAACCGGGCCTACGC1446060.15618771078593086No Hit
AAAAAGTTACGGTAGGTTATTCCTTGGAGTCGGGTTGTTTGAGATTGCAG1403580.15159948211340943No Hit
CCCTTACCTACATTATTCTATCGACTAGAGGCTGTTCACCTTGGAGACCT1382220.14929240667920374No Hit
CCCCAGTCAAACTCCCCGCCTGACACTGTCTTCGGTGCGAGTCGTTAAAT1372730.14826739985005521No Hit
CCTCGTTAACAGGTTTAAATTGTACCCATTCCGATAACCGGCCTCAAAGA1306120.14107291039909825No Hit
GTCGTCTGCAAGTGATCTTGCGCCCCGACATTGTACCAGAGTAATCCGGT1305140.14096706143254759No Hit
CAGCTTTGCAACCATACTTCCCCCGGAACCGAAAAACTTTCGTTTCCGGG1289930.13932424226802193No Hit
GTCGGCGGTTATATGCTTGTCTCAAAGGCTAAGCCATGCATGTCTAAGTT1277330.13796332698379946No Hit
GGGGCATTAGCTGTAATAGTATGAATATGTTCATAATTACATAATAGTGC1266170.13675794487491671No Hit
GTGATTTGTCTGGTTTATTCCGATAACGAGCGAGACTCTAGCCTACTAAA1233380.13321632486145207No Hit
GGCCGTTCTTAGTTGGTGGAGTGATTTGTCTGGTTTATTCCGATAACGAG1228020.13263739581990983No Hit
ATTAGATTAAAACCAATCGGATTATTAATTTTAATCCGTTAATTGGTGAC1194740.12904285132316987No Hit
GCGGTGTGTACAAAGGGCAGGGACGTAATCAACGTGAGCTGATGACTCAC1193260.1288829977818485No Hit
CTAGCATATACGCAAGTTATTGGGTAGTAAACCTAATTTAAAAGATAAAA1187770.12829002755086585No Hit
CGCCCTCTTGAACTCTCTCTTCAAAGTTCTTTGCAACTTTCCCTCACGGT1180440.1274983204847269No Hit
GCGTAACATAACCCCGTTCATGTTGCATGCAATAGGGCGGAGGTAGAGCG1155710.1248272542165665No Hit
GCCAGTTCTAAGTTGACTGTTTAACGCCGGCCGAAATATCAAATAAAACA1143140.12346957920683027No Hit
CCCGAAGTTACGGATCTAATTTGCCGACTTCCCTTACCTACATTATTCTA1140850.1232222382543803No Hit
CGCGCGTGCAGCCCTGGACATCTAAGGGCATCACAGACCTGTTATTGCTC1131970.12226311700645211No Hit
GCGAGTCGTTAAATCACCGAGTGATTTAACTTAAATCAAAAATTTCGAAA1113270.12024334591002671No Hit
GGAATAATGAAATAGGATCTCGGTTCTATTTTGTTGGTTTTCTGATCTGA1093340.11809072355966532No Hit
GAAATTCTTGGACCGTAGCGAGACGTACGACTGCGAAAGCATTTGCCAAG1078410.11647814695701125No Hit
CTCTGACTTTCGTTCTTGATTAATGAAGACATTCTTGGCAAATGCTTTCG1065160.11504702572558684No Hit
CTCCACTTCAGTCTTCAAGGATCTCACTTGAATATTTGCTACTACCACCA1059190.1144022111028243No Hit
CGGAAACCTCTCGTAGAGTAAAAGGGCAAAAGCTCGCTTGATCTTGATTT1047130.11309962075935422No Hit
GCGGATATGGGTACGATCTGGCACGAAATTCAAATAGCTTCCCTCGGATT1033200.11159505330624162No Hit
CCCGTCCGTCCCTCTTAACCATTATCTCAGATCAGAAAACCAACAAAATA1031230.11138227528164493No Hit
GGAGTCTAGCATATACGCAAGTTATTGGGTAGTAAACCTAATTTAAAAGA1024640.11067049498616668No Hit
ACGAAATTCAAATAGCTTCCCTCGGATTTTCATGGATCGAACAAAGCGCA1024520.1106575338882217No Hit
CTCTTAACCATTATCTCAGATCAGAAAACCAACAAAATAGAACCGAGATC1021960.11038103046539555No Hit
CCCGCATTGAGCACTCTAATTTTTTCAAGGTAAACTTGCTAGCCACCATA1021180.11029678332875321No Hit
ATGAAATAGGATCTCGGTTCTATTTTGTTGGTTTTCTGATCTGAGATAAT1016090.10974701675758715No Hit
GTTACGGTAGGTTATTCCTTGGAGTCGGGTTGTTTGAGATTGCAGCCCAA1014500.10957528220981623No Hit
GTACGGGTGAAAAAAGTTACGGTAGGTTATTCCTTGGAGTCGGGTTGTTT1013740.10949319525616472No Hit
GTTGCGATAGTAATCCTGCTCAGTACGAGAGGAACCGCAGGTTCAGACAT1004660.1085124721783282No Hit
GTGCGAGTCGTTAAATCACCGAGTGATTTAACTTAAATCAAAAATTTCGA997500.1077391266676113No Hit
GCGCGCCTGCTGCCTTCCTTGGATGTGGTAGCCGTTTCTCAGGCTCCCTC993310.10728656833103258No Hit
GGAAGTTGGAAACCGCTAAGGAGTGTGTAACAACTCACCTGCCAAATCAA991110.10704894820204136No Hit
GTGAAATTCTTGGACCGTAGCGAGACGTACGACTGCGAAAGCATTTGCCA944620.10202760283985865No Hit
GTGAGGATTGACAGATTGAGAGCTCTTTCATGATTCGGTGGTTGGTGGTG944490.10201356165041825No Hit
GGCACTTTAACTCAACGTTTGGTTCATCCCACAGCGCCAGTTCTGCTTAC933970.10087730539724205No Hit
GTCAATTTCTTTAAGTTTCAGCTTTGCAACCATACTTCCCCCGGAACCGA932330.10070017039199404No Hit
GCACTCTAATTTTTTCAAGGTAAACTTGCTAGCCACCATATGCAACCAAT931270.10058568069348009No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCTGGCC723750.072.7909855
ACCTGGC779650.067.731444
CACCTGG808400.065.34913
GTTAGTT1720800.064.468621
TGGCCCG833050.063.0208477
CTGGCCC838850.062.7803736
AGAACCC204100.062.57705145
TAGTTTT1790300.061.9344643
GGCGGTT333450.060.055254
TTAGTTT1850200.059.9720732
GAGTCTA265700.059.7567062
GCCCGGA903000.059.7172439
GGAGTCT281350.056.4028471
AGTTTTA2024400.055.1920054
CCAGACG257250.054.271716
TTACCCT2045350.053.939158
AGTCTAG287200.053.2650343
TACCCTA2093250.052.8489389
GCCTTGT1855350.052.5531351
GGTTATA384400.052.5216837
Produced by FastQC (version 0.11.2)