FastQCFastQC Report
Wed 14 Dec 2016
HVMVCBGXY_n01_brugia6-l3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHVMVCBGXY_n01_brugia6-l3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences76568470
Sequences flagged as poor quality0
Sequence length151
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTTAGTTTTACCCTACTGTGATATGTTGTTGCGATAGTAATCCTGCTCAG11853761.5481254882068298No Hit
CTGCAAGTGATCTTGCGCCCCGACATTGTACCAGAGTAATCCGGTTTTGG8690521.1349998243402277No Hit
GTTTTACCCTACTGTGATATGTTGTTGCGATAGTAATCCTGCTCAGTACG7274380.9500490214836472No Hit
CCGACATCGAAGGATCAAAAAGCAACGTCGCTATGAACGCTTGGCTGCCA5005600.6537416772204016No Hit
CTCACCTGGCCCGGACACCGTGAGGATTGACAGATTGAGAGCTCTTTCAT2960160.38660299729118264No Hit
GCCTTGTCTAGGTGGGATTCTGACTTAGAGGCGTTCAGTCATAATCCCTC2714540.3545245190350545No Hit
GCCAGTTCTAAGTTGACTGTTTAACGCCGGCCGAAATATCAAATAAAACA2489350.32511424088792684No Hit
GCAAGTGATCTTGCGCCCCGACATTGTACCAGAGTAATCCGGTTTTGGCA2381260.3109974640997789No Hit
GTGATTTGTCTGGTTTATTCCGATAACGAGCGAGACTCTAGCCTACTAAA2278960.2976368732456062No Hit
CGCGCGTGCAGCCCTGGACATCTAAGGGCATCACAGACCTGTTATTGCTC2277840.2974905989371343No Hit
GCGGTGTGTACAAAGGGCAGGGACGTAATCAACGTGAGCTGATGACTCAC2253780.2943483133462116No Hit
AAGTGATCTTGCGCCCCGACATTGTACCAGAGTAATCCGGTTTTGGCAAC2219920.2899261275561599No Hit
CTTGTCTAGGTGGGATTCTGACTTAGAGGCGTTCAGTCATAATCCCTCAG2157100.28172170607562097No Hit
GTCGTCTGCAAGTGATCTTGCGCCCCGACATTGTACCAGAGTAATCCGGT2151920.2810451873989385No Hit
CTAGCATATACGCAAGTTATTGGGTAGTAAACCTAATTTAAAAGATAAAA2143320.27992200967317227No Hit
GTCTGCAAGTGATCTTGCGCCCCGACATTGTACCAGAGTAATCCGGTTTT2124360.2774457945940411No Hit
GCGATAGTAATCCTGCTCAGTACGAGAGGAACCGCAGGTTCAGACATTTG2103940.274778900505652No Hit
CCCGAGTCCGTGCCAGTTCTAAGTTGACTGTTTAACGCCGGCCGAAATAT2007400.2621705775236204No Hit
TGGAAATCAAGATCAAGCGAGCTTTTGCCCTTTTACTCTACGAGAGGTTT1959930.25597089768151304No Hit
GGCATTAGCTGTAATAGTATGAATATGTTCATAATTACATAATAGTGCCT1859020.2427918436923188No Hit
GTGATCTTGCGCCCCGACATTGTACCAGAGTAATCCGGTTTTGGCAACCA1847010.24122331293808014No Hit
CAGAAATCAGAGACAGTCAACGCCCGCAAGGGCCATCTGTCTCCTCTGTT1844980.24095819075397482No Hit
GTTGCGATAGTAATCCTGCTCAGTACGAGAGGAACCGCAGGTTCAGACAT1775490.23188265352566143No Hit
GTACAAAGGGCAGGGACGTAATCAACGTGAGCTGATGACTCACACTTACT1759170.2297512278879283No Hit
GACAGGTTAGTTTTACCCTACTGTGATATGTTGTTGCGATAGTAATCCTG1742310.22754927713718193No Hit
CTGTAATAGTATGAATATGTTCATAATTACATAATAGTGCCTCCCGACAT1715860.22409485262014509No Hit
CAAAGGGCAGGGACGTAATCAACGTGAGCTGATGACTCACACTTACTAGG1631720.213105995196195No Hit
CTGACTTAGAGGCGTTCAGTCATAATCCCTCAGATGGTAGCTTCGCACCA1584320.20691545749836715No Hit
CTCCAGTGTAGCGCGCGTGCAGCCCTGGACATCTAAGGGCATCACAGACC1562560.20407355664805632No Hit
AGACAGGTTAGTTTTACCCTACTGTGATATGTTGTTGCGATAGTAATCCT1515450.1979208935479578No Hit
GGCGAATTCTGCTTCGCAATGATAGGAAGAGCCGACATCGAAGGATCAAA1465250.19136467007895025No Hit
CGGAAACCTCTCGTAGAGTAAAAGGGCAAAAGCTCGCTTGATCTTGATTT1463350.1911165261627926No Hit
CGTCTGCAAGTGATCTTGCGCCCCGACATTGTACCAGAGTAATCCGGTTT1425820.1862150308083732No Hit
GCGAAACCACAGCCAAGGGAACGGGCTTGGCAAACACAGCGGGGAAAGAA1363280.17804717790495225No Hit
CCCCAGTCAAACTCCCCGCCTGACACTGTCTTCGGTGCGAGTCGTTAAAT1344220.17555790261970758No Hit
CTCTGTTTTAATTAGACAGTCGGATTCCCCGAGTCCGTGCCAGTTCTAAG1330600.17377910254704057No Hit
TGCAAGTGATCTTGCGCCCCGACATTGTACCAGAGTAATCCGGTTTTGGC1300350.16982839019768844No Hit
CTGGAAATCAAGATCAAGCGAGCTTTTGCCCTTTTACTCTACGAGAGGTT1300120.16979835172362723No Hit
CCCGAAGTTACGGATCTAATTTGCCGACTTCCCTTACCTACATTATTCTA1295190.16915448356222867No Hit
CTCCACTCCTGGTGGTGCCCTTCCGTCAATTTCTTTAAGTTTCAGCTTTG1286850.1680652623723577No Hit
GTCAAAACGTAACGCAGGTGTCCTAAGGCTAGCTCAGAGAGGACGGAAAC1281360.1673482570567232No Hit
CTGGCCCGGACACCGTGAGGATTGACAGATTGAGAGCTCTTTCATGATTC1227360.16029574575540037No Hit
CCCGTCCGTCCCTCTTAACCATTATCTCAGATCAGAAAACCAACAAAATA1211450.15821786696273282No Hit
CTGGTTTATTCCGATAACGAGCGAGACTCTAGCCTACTAAATAGTTACTG1156560.15104911982699928No Hit
GCGAGTCGTTAAATCACCGAGTGATTTAACTTAAATCAAAAATTTCGAAA1154490.15077877356044858No Hit
GTCAAACTCCCCGCCTGACACTGTCTTCGGTGCGAGTCGTTAAATCACCG1141670.14910445513669007No Hit
GCCTGACACTGTCTTCGGTGCGAGTCGTTAAATCACCGAGTGATTTAACT1139040.1487609717159034No Hit
GTTTTAATTAGACAGTCGGATTCCCCGAGTCCGTGCCAGTTCTAAGTTGA1136470.1484253244187849No Hit
CAGCTTTGCAACCATACTTCCCCCGGAACCGAAAAACTTTCGTTTCCGGG1134120.14811840957511624No Hit
CTTGATTAATGAAGACATTCTTGGCAAATGCTTTCGCAGTCGTACGTCTC1129250.1474823775373858No Hit
GGAGTCTAGCATATACGCAAGTTATTGGGTAGTAAACCTAATTTAAAAGA1125780.14702918838524526No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGCTCATTATCTCGTAT1115100.14563435837231695TruSeq Adapter, Index 2 (97% over 37bp)
CGAGAATTGAAATATTATATGTATTTCGAAATTTTTGATTTAAGTTAAAT1077550.14073025097667485No Hit
GCGAGACTCTAGCCTACTAAATAGTTACTGGATAATTTTATCGTCCAGAC1065380.1391408238926545No Hit
GCAAGTTATTGGGTAGTAAACCTAATTTAAAAGATAAAAAGCATAATGAA1051920.13738292014976922No Hit
CTTGTTACGACTTTTGCCCGGTTCAAGCCACTGCGATTAAAGCAGTTTCC1044610.13642821908286792No Hit
CTCGTTAACAGGTTTAAATTGTACCCATTCCGATAACCGGCCTCAAAGAG1043390.13626888456828248No Hit
CGCCCCTATACCAAAGTCAGACGATCGATTTGCACGTCAGAATCGCTTCG1027690.13421843220845345No Hit
CTTGACTCTAGTCCGACTCTGTGAAGAGTCATGAGAGGTGTAGCATAAGT1027200.13415443719849698No Hit
GATTTGTCTGGTTTATTCCGATAACGAGCGAGACTCTAGCCTACTAAATA1025980.13399510268391154No Hit
CTGCTCTACTGCTTACAACACCTTGACCAGTAATCAAGTCGTCTGCAAGT1006130.1314026517703697No Hit
TAGTAATCCTGCTCAGTACGAGAGGAACCGCAGGTTCAGACATTTGGTTC996390.13013058769490887No Hit
GTGTACAAAGGGCAGGGACGTAATCAACGTGAGCTGATGACTCACACTTA988240.12906618089665367No Hit
CCCTTACCTACATTATTCTATCGACTAGAGGCTGTTCACCTTGGAGACCT978110.12774318201734997No Hit
CCCGCCTGACACTGTCTTCGGTGCGAGTCGTTAAATCACCGAGTGATTTA977180.12762172210049383No Hit
CTTAGATGTCCAGGGCTGCACGCGCGCTACACTGGAGGAATCAGCGTGCT975410.12739055645228384No Hit
CAGGGACGTAATCAACGTGAGCTGATGACTCACACTTACTAGGAATTCCT962530.1257084019048572No Hit
GTCTAGGTGGGATTCTGACTTAGAGGCGTTCAGTCATAATCCCTCAGATG958230.12514681304197406No Hit
GCGCGTGCAGCCCTGGACATCTAAGGGCATCACAGACCTGTTATTGCTCA936190.12226834361454525No Hit
GGAAATCAAGATCAAGCGAGCTTTTGCCCTTTTACTCTACGAGAGGTTTC910600.11892623687008504No Hit
GGGGCATTAGCTGTAATAGTATGAATATGTTCATAATTACATAATAGTGC908340.11863107621191855No Hit
GACAGATTGAGAGCTCTTTCATGATTCGGTGGTTGGTGGTGCATGGCCGT891400.11641867729628136No Hit
GCCGTTCTTAGTTGGTGGAGTGATTTGTCTGGTTTATTCCGATAACGAGC885950.1157068960630923No Hit
CTCTTAACCATTATCTCAGATCAGAAAACCAACAAAATAGAACCGAGATC874460.11420627838064415No Hit
CCTCGTTCAAGGGAAATAATTGCAATTCCCGATCCCGATCACGGAGGATT871030.11375831331094902No Hit
CGAGACTCTAGCCTACTAAATAGTTACTGGATAATTTTATCGTCCAGACA866780.11320325455112268No Hit
CTCCGTGTCCAGGTACGGGAATATTAACCCGTTTCCCTTTCGATTTATCA862460.11263905364701686No Hit
GTCCAATCAAAGGATAAGTCTCAACAGATCGCAGTATGGAGGCTGCTCTA855690.11175487769312878No Hit
GTGAAATACCACTACTTTCATCGATTCTTTACTTATTCGGTAAAACGAGA854060.11154199633347772No Hit
CCCCGAGTCCGTGCCAGTTCTAAGTTGACTGTTTAACGCCGGCCGAAATA851510.11120896107758194No Hit
CCGCCTGACACTGTCTTCGGTGCGAGTCGTTAAATCACCGAGTGATTTAA824650.10770098971547949No Hit
CAAACGTTGAGTTAAAGTGCCAAACTTTCCGCTCATGAGACCCCATGAAA820120.10710936237853519No Hit
CGATAACGAGCGAGACTCTAGCCTACTAAATAGTTACTGGATAATTTTAT819100.10697614827617688No Hit
CTCCCACTTATGCTACACCTCTCATGACTCTTCACAGAGTCGGACTAGAG816930.10669274180351258No Hit
GGCTGCTCTACTGCTTACAACACCTTGACCAGTAATCAAGTCGTCTGCAA801610.10469191822691508No Hit
GTGTGTACAAAGGGCAGGGACGTAATCAACGTGAGCTGATGACTCACACT799280.1043876154244691No Hit
GGAACCGCAGGTTCAGACATTTGGTTCATGTGCTTGGCCGATCGGCCAAT776850.10145821119319741No Hit
CTTGCTAGAAACCTTCTAAAAAATTCAAAAGGATCGATAGGCCACGCTTT771470.10075557210428784No Hit
ATTGAGAGCTCTTTCATGATTCGGTGGTTGGTGGTGCATGGCCGTTCTTA766960.10016655680856623No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACCTGGC523800.083.74334
GTTAGTT2130850.083.47421
CCGACAA136600.083.38522145
CACCTGG527050.083.310473
GTCCAAT165600.079.655431
TTAGTTT2251550.079.082412
AGTTTTA2307500.077.3485954
CCTGGCC567800.077.3304145
CTTGTTA212600.077.190581
TAGTTTT2318050.076.941223
TCCAATC171800.076.501942
GGAGTCT227100.074.88051
GAGTCTA239150.071.144362
GGCCGAC41750.067.046151
AGTCTAG258050.066.04543
GTTACGA253750.064.7068944
CTGGCCC708750.061.9514546
TTACGAC266200.061.6533245
CTAGCAT538700.060.4694181
GCGAGCG66900.060.377081
Produced by FastQC (version 0.11.2)