FastQCFastQC Report
Wed 14 Dec 2016
HVMVCBGXY_n01_brugia1-l3d9.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHVMVCBGXY_n01_brugia1-l3d9.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences92584749
Sequences flagged as poor quality0
Sequence length151
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTTAGTTTTACCCTACTGTGATATGTTGTTGCGATAGTAATCCTGCTCAG7737280.835697032564186No Hit
GCCTTGTCTAGGTGGGATTCTGACTTAGAGGCGTTCAGTCATAATCCCTC6288980.6792673812832825No Hit
GTTTTACCCTACTGTGATATGTTGTTGCGATAGTAATCCTGCTCAGTACG3913170.42265816371117454No Hit
CTCACCTGGCCCGGACACCGTGAGGATTGACAGATTGAGAGCTCTTTCAT3859930.41690775658958695No Hit
GCCCTCTTGAACTCTCTCTTCAAAGTTCTTTGCAACTTTCCCTCACGGTA3766080.40677109790512045No Hit
CTCTCTTCAAAGTTCTTTGCAACTTTCCCTCACGGTACTTGTTTGCTATC3259950.3521044270476988No Hit
CTCCAGTGTAGCGCGCGTGCAGCCCTGGACATCTAAGGGCATCACAGACC2870940.31008778778457347No Hit
CTTGTCTAGGTGGGATTCTGACTTAGAGGCGTTCAGTCATAATCCCTCAG2629340.28399277725535554No Hit
CTTGATTAATGAAGACATTCTTGGCAAATGCTTTCGCAGTCGTACGTCTC2590980.2798495462789449No Hit
CAAACGTTGAGTTAAAGTGCCAAACTTTCCGCTCATGAGACCCCATGAAA2577030.2783428186428415No Hit
GAAAAAAGTTACGGTAGGTTATTCCTTGGAGTCGGGTTGTTTGAGATTGC2569780.27755975230866586No Hit
CTCGTTAACAGGTTTAAATTGTACCCATTCCGATAACCGGCCTCAAAGAG2549970.2754200910562494No Hit
CCTTATTCTCCGTTACCCGTTACAACCATGGTAGGCACATAAACTACCAT2488440.2687742880849631No Hit
CTGCAAGTGATCTTGCGCCCCGACATTGTACCAGAGTAATCCGGTTTTGG2468550.26662598610058336No Hit
GTACGATCTGGCACGAAATTCAAATAGCTTCCCTCGGATTTTCATGGATC2366890.25564577595819804No Hit
CTCCGTTACCCGTTACAACCATGGTAGGCACATAAACTACCATCGAAAGT2362270.2551467736873165No Hit
AAAAAAGTTACGGTAGGTTATTCCTTGGAGTCGGGTTGTTTGAGATTGCA2193000.23686406494443268No Hit
GTCAGATAGGTGATTAACGTTCACATCGAGATGTAACTTTATTGCATACA2185370.23603995513343132No Hit
CCGACATCGAAGGATCAAAAAGCAACGTCGCTATGAACGCTTGGCTGCCA2148490.2320565776983421No Hit
CTCCGTGTCCAGGTACGGGAATATTAACCCGTTTCCCTTTCGATTTATCA2015880.2177334843776484No Hit
GCGCCTGCTGCCTTCCTTGGATGTGGTAGCCGTTTCTCAGGCTCCCTCTC1963010.21202304064139116No Hit
CTCGCTCGTTATCGGAATAAACCAGACAAATCACTCCACCAACTAAGAAC1822860.19688555833315488No Hit
CCCTTACCTACATTATTCTATCGACTAGAGGCTGTTCACCTTGGAGACCT1763520.1904762953993643No Hit
CGCGCCTGCTGCCTTCCTTGGATGTGGTAGCCGTTTCTCAGGCTCCCTCT1747850.18878379202604956No Hit
GTGAAATACCACTACTTTCATCGATTCTTTACTTATTCGGTAAAACGAGA1722200.186013357340311No Hit
ATTAAAACCAATCGGATTATTAATTTTAATCCGTTAATTGGTGACTCTGA1642670.17742338967727828No Hit
CCCCAGTCAAACTCCCCGCCTGACACTGTCTTCGGTGCGAGTCGTTAAAT1639590.17709072149669056No Hit
GACAGGTTAGTTTTACCCTACTGTGATATGTTGTTGCGATAGTAATCCTG1550360.16745306508310565No Hit
CCTCGTTAACAGGTTTAAATTGTACCCATTCCGATAACCGGCCTCAAAGA1541000.16644209944339752No Hit
GGCATTAGCTGTAATAGTATGAATATGTTCATAATTACATAATAGTGCCT1479140.1597606534527625No Hit
GCCAGTTCTAAGTTGACTGTTTAACGCCGGCCGAAATATCAAATAAAACA1434580.15494776574919483No Hit
GTCTGGTGCCAGCAGCCGCGGTAATTCCAGCTCTCAAAGTGTATATCGTC1423070.15370458043797258No Hit
CCCGAAGTTACGGATCTAATTTGCCGACTTCCCTTACCTACATTATTCTA1346060.14538679583178435No Hit
AAAAAGTTACGGTAGGTTATTCCTTGGAGTCGGGTTGTTTGAGATTGCAG1344060.1451707775327014No Hit
CTAGCATATACGCAAGTTATTGGGTAGTAAACCTAATTTAAAAGATAAAA1327330.14336378446087272No Hit
GTCGTCTGCAAGTGATCTTGCGCCCCGACATTGTACCAGAGTAATCCGGT1314780.14200826963412733No Hit
CTCTGACTTTCGTTCTTGATTAATGAAGACATTCTTGGCAAATGCTTTCG1314360.14196290579131993No Hit
GTGATTTGTCTGGTTTATTCCGATAACGAGCGAGACTCTAGCCTACTAAA1309920.1414833451673558No Hit
CAGCTTTGCAACCATACTTCCCCCGGAACCGAAAAACTTTCGTTTCCGGG1292910.1396461095336555No Hit
CGCCCTCTTGAACTCTCTCTTCAAAGTTCTTTGCAACTTTCCCTCACGGT1290100.13934260382344396No Hit
CTCTTAACCATTATCTCAGATCAGAAAACCAACAAAATAGAACCGAGATC1277560.137988169088194No Hit
ATTAGATTAAAACCAATCGGATTATTAATTTTAATCCGTTAATTGGTGAC1245340.13450811428996798No Hit
CGCGCGTGCAGCCCTGGACATCTAAGGGCATCACAGACCTGTTATTGCTC1191010.1286399771953802No Hit
GCGTAACATAACCCCGTTCATGTTGCATGCAATAGGGCGGAGGTAGAGCG1189120.12843583990274685No Hit
CGGAAACCTCTCGTAGAGTAAAAGGGCAAAAGCTCGCTTGATCTTGATTT1162990.12561356082522837No Hit
CCCGCATTGAGCACTCTAATTTTTTCAAGGTAAACTTGCTAGCCACCATA1159870.125276572278659No Hit
GGAATAATGAAATAGGATCTCGGTTCTATTTTGTTGGTTTTCTGATCTGA1147940.1239880231246293No Hit
CCCGAGTCCGTGCCAGTTCTAAGTTGACTGTTTAACGCCGGCCGAAATAT1132810.12235384469206695No Hit
GTCGGCGGTTATATGCTTGTCTCAAAGGCTAAGCCATGCATGTCTAAGTT1130020.12205249916484626No Hit
ACGAAATTCAAATAGCTTCCCTCGGATTTTCATGGATCGAACAAAGCGCA1104860.119334988962383No Hit
GCGAGTCGTTAAATCACCGAGTGATTTAACTTAAATCAAAAATTTCGAAA1097270.11851519951736326No Hit
ATGAAATAGGATCTCGGTTCTATTTTGTTGGTTTTCTGATCTGAGATAAT1090350.11776777620253634No Hit
GCGGTGTGTACAAAGGGCAGGGACGTAATCAACGTGAGCTGATGACTCAC1086600.11736274189175584No Hit
CCCGTCCGTCCCTCTTAACCATTATCTCAGATCAGAAAACCAACAAAATA1081430.11680433458862646No Hit
GAAATTCTTGGACCGTAGCGAGACGTACGACTGCGAAAGCATTTGCCAAG1063760.11489581291622879No Hit
CTCAACCAGACGTACCATCGGGAATCAACCCAATGGTGCAATGTGCATTC1046000.11297757042037238No Hit
CTCCCACTTATGCTACACCTCTCATGACTCTTCACAGAGTCGGACTAGAG1045070.1128771219112988No Hit
GGCCGTTCTTAGTTGGTGGAGTGATTTGTCTGGTTTATTCCGATAACGAG1032750.11154644918894795No Hit
GTTACGGTAGGTTATTCCTTGGAGTCGGGTTGTTTGAGATTGCAGCCCAA1030380.11129046750453467No Hit
GTTGCGATAGTAATCCTGCTCAGTACGAGAGGAACCGCAGGTTCAGACAT1027730.11100424325824979No Hit
CGCAAATGAACTCGGAAACATTGAGGAAAGCCCGAGCCAGAGTTCTCTTT1022570.11044691604661584No Hit
GTCAATTTCTTTAAGTTTCAGCTTTGCAACCATACTTCCCCCGGAACCGA1016620.10980426160684413No Hit
CCCTGTGGTAACTTTTCTGACACCTCTTGCTAGAAACCTTCTAAAAAATT1010990.10919617009492567No Hit
GGAAGTTGGAAACCGCTAAGGAGTGTGTAACAACTCACCTGCCAAATCAA1002210.1082478497619516No Hit
GTGAAATTCTTGGACCGTAGCGAGACGTACGACTGCGAAAGCATTTGCCA1001430.10816360262530927No Hit
GCACTCTAATTTTTTCAAGGTAAACTTGCTAGCCACCATATGCAACCAAT990980.10703490701260096No Hit
GGAGTCTAGCATATACGCAAGTTATTGGGTAGTAAACCTAATTTAAAAGA990440.10697658207184857No Hit
GTACGGGTGAAAAAAGTTACGGTAGGTTATTCCTTGGAGTCGGGTTGTTT981730.1060358223793424No Hit
CTTAGATGTCCAGGGCTGCACGCGCGCTACACTGGAGGAATCAGCGTGCT969190.10468138764409243No Hit
GGCACTTTAACTCAACGTTTGGTTCATCCCACAGCGCCAGTTCTGCTTAC962850.10399660963599955No Hit
CTCTGTTTTAATTAGACAGTCGGATTCCCCGAGTCCGTGCCAGTTCTAAG962600.1039696073486142No Hit
CGCCCCTATACCAAAGTCAGACGATCGATTTGCACGTCAGAATCGCTTCG961770.10387995975449478No Hit
CGCCGACGGAACGCTAGTTGGTATCGTTTACGGTCAGAACTAGGGCGGTA958860.10356565312932911No Hit
CCCACTTGGAGCACACATTCAATGTCTATGCTTCATAAAAAATTTAAGCA957160.10338203757510862No Hit
GCGGATATGGGTACGATCTGGCACGAAATTCAAATAGCTTCCCTCGGATT956130.10327078815108091No Hit
CCCGTTACAACCATGGTAGGCACATAAACTACCATCGAAAGTTGATAAGG954340.10307745177340168No Hit
CTTGAACTCTCTCTTCAAAGTTCTTTGCAACTTTCCCTCACGGTACTTGT942600.10180942435778488No Hit
GTGCGAGTCGTTAAATCACCGAGTGATTTAACTTAAATCAAAAATTTCGA936140.10111168525174703No Hit
CAACGGTTGAGTATAAGTCTCCCGCTCAAGCGCCATCCATTTTCAGGGCT931570.10061808343834254No Hit
GGGGCATTAGCTGTAATAGTATGAATATGTTCATAATTACATAATAGTGC930010.10044958916505785No Hit
CTTCCGTCAATTTCTTTAAGTTTCAGCTTTGCAACCATACTTCCCCCGGA926600.10008127796512145No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCTGGCC780400.075.538875
ACCTGGC834700.070.8684544
CACCTGG865050.068.39933
GTTAGTT1749050.066.959471
AGAACCC114750.065.77476145
TAGTTTT1785600.065.688693
CTGGCCC901400.065.358596
GAGTCTA246700.065.029952
GCCCGGA923700.064.96299
GGCGGTT274950.063.673194
TTAGTTT1862950.062.993132
AGTCTAG248350.061.3279083
CCAGACG265100.059.3663066
TGGCCCG1004900.058.6820077
GGAGTCT274950.058.4463961
AGTTTTA2018550.058.409174
GGTTATA306500.057.068587
GTCGTCT359650.056.3765951
TTACCCT2108100.055.509078
TACCCTA2150700.054.439919
Produced by FastQC (version 0.11.2)