FastQCFastQC Report
Wed 16 Oct 2019
HVMTNAFXY_n02_undetermined.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHVMTNAFXY_n02_undetermined.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17717107
Sequences flagged as poor quality0
Sequence length124
%GC48

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG6071193.4267389139773217No Hit
GGGGTGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG278930.1574354097426854No Hit
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT187530.10584685185905353No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCTCTGA1412700.0116.594859
TGCTGTA1495200.0116.550642
TAGCTCT1500050.0116.1451957
GCTGTAG1526050.0116.144123
AGCTCTG1534150.0116.132528
GTAGCTC1519300.0116.0041666
CTAACGG106950.0113.275636
CTAACTC161850.0111.495936
TAACGGC110350.0110.920397
GACGTGG140250.0110.568085
CTGTCTA108800.0109.900862
GCTCTGG261750.0109.853599
ACTGTCT108400.0109.091961
ACGTGGT146850.0109.008036
CTGTAGC1633450.0108.858054
GTGGTCC146700.0108.8124548
TCTAACG114250.0108.460435
CTCTAAC181950.0108.351694
TGTAGCT1635400.0108.27995
CAATGGG61300.0108.02853118
Produced by FastQC (version 0.11.5)