FastQCFastQC Report
Wed 16 Oct 2019
HVMTNAFXY_n02_cDNA_04.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHVMTNAFXY_n02_cDNA_04.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3227988
Sequences flagged as poor quality0
Sequence length124
%GC49

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT2496777.734756139118238No Hit
GCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT479251.484670946732144No Hit
AGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT223830.6934040646991253No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG110220.34145108346127684No Hit
CAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT78240.24238008319733528No Hit
AGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTT68730.21291900713385553No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT66250.20523620286072936No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGCAGT301000.0109.374381
AGCAGTG323450.0101.744572
CAGTGGT393650.083.4474954
GCAGTGG395550.082.973
AGTGGTA397350.082.410345
GTATCAA406850.081.032789
TGGTATC406050.080.720547
GGTATCA410100.080.670168
GTGGTAT408950.080.104866
TAAGGGC10700.044.665268118
ATCAACG402100.044.25241510-11
CAACGCA407350.043.89307812-13
ACGCAGA408650.043.67991314-15
AGAGTAC413750.043.47244318-19
GCAGAGT416200.043.03836416-17
AGTACTT421950.042.30671720-21
TACTTTT433050.041.223922-23
TCAACGC404700.040.8279612-13
AACGCAG408600.040.3720514-15
TATCAAC411150.040.27261710-11
Produced by FastQC (version 0.11.5)