FastQCFastQC Report
Wed 16 Oct 2019
HVMTNAFXY_n02_cDNA_03.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHVMTNAFXY_n02_cDNA_03.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5692582
Sequences flagged as poor quality0
Sequence length124
%GC50

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT4064047.139185698159464No Hit
GCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT795641.3976785929478046No Hit
AGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT363740.638971911164389No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG221020.3882596684597604No Hit
CAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT127350.2237121924638064No Hit
AGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTT107030.1880166153074299No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT106920.18782338137597315No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGCAGT506500.0108.382821
AGCAGTG550350.099.6479342
CAGTGGT656150.083.383754
AGTGGTA662650.082.180275
GCAGTGG668150.081.902533
GTATCAA678050.080.921799
GGTATCA686600.080.368618
TGGTATC679450.080.3301547
GTGGTAT683700.079.6976556
ATCAACG671600.044.7177810-11
CAACGCA680850.044.52273612-13
ACGCAGA684950.044.2601514-15
AGAGTAC692850.044.15427418-19
GCAGAGT702350.043.3289416-17
AGTACTT710800.042.7544220-21
TACTTTT730650.041.49378622-23
TCAACGC678700.040.7875312-13
TATCAAC684400.040.4469410-11
AACGCAG686150.040.2970314-15
GAGTACT700700.039.64947520-21
Produced by FastQC (version 0.11.5)