FastQCFastQC Report
Wed 16 Oct 2019
HVMTNAFXY_n02_cDNA_02.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHVMTNAFXY_n02_cDNA_02.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4550326
Sequences flagged as poor quality0
Sequence length124
%GC49

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT3432947.5443825343502855No Hit
GCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT669241.470751765917431No Hit
AGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT306990.6746549587875682No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG162630.35740296409531974No Hit
CAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT106750.23459857601411416No Hit
AGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTT95210.20923775571244785No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT90030.19785395595832037No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGCAGT417650.0109.100521
AGCAGTG457500.099.5056462
CAGTGGT549350.082.596154
AGTGGTA558150.080.8524865
GCAGTGG561250.080.843573
GTATCAA571050.079.658719
TGGTATC569450.079.374917
GGTATCA575350.079.360718
GTGGTAT575250.078.503686
ATCAACG566900.043.87234510-11
CAACGCA574150.043.6660112-13
AGAGTAC580800.043.483318-19
ACGCAGA576300.043.42050614-15
GCAGAGT586150.042.81852316-17
AGTACTT597700.041.92338620-21
TACTTTT610650.041.03892522-23
TCAACGC572400.039.98122412-13
AACGCAG576300.039.6485914-15
TATCAAC578200.039.622510-11
GTACTTT585750.038.94625522-23
Produced by FastQC (version 0.11.5)