FastQCFastQC Report
Wed 16 Oct 2019
HVMTNAFXY_n02_cDNA_01.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHVMTNAFXY_n02_cDNA_01.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4520300
Sequences flagged as poor quality0
Sequence length124
%GC49

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT3487437.715041037099307No Hit
GCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT670371.4830210384266531No Hit
AGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT310950.6878968210074552No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG159710.3533172577041347No Hit
CAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT109330.24186447802137023No Hit
AGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTT96520.21352565095237044No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT92050.20363692675264916No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGCAGT424950.0109.510721
AGCAGTG465650.099.836142
CAGTGGT557450.083.1843644
GCAGTGG564400.082.112313
AGTGGTA565950.081.719315
GTATCAA580700.080.1298459
GGTATCA584700.079.835638
TGGTATC581650.079.6078957
GTGGTAT584250.079.1965566
ATCAACG575600.044.16085410-11
CAACGCA581800.043.931912-13
AGAGTAC589800.043.70517318-19
ACGCAGA584350.043.68491414-15
GCAGAGT594550.043.0720216-17
AGTACTT602700.042.40313320-21
TACTTTT619050.041.18881622-23
TCAACGC579950.040.1205112-13
TATCAAC585050.039.8735510-11
AACGCAG584500.039.7327214-15
GTACTTT592650.039.1811422-23
Produced by FastQC (version 0.11.5)