FastQCFastQC Report
Wed 16 Oct 2019
HVMTNAFXY_n02_HTO.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHVMTNAFXY_n02_HTO.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences29381660
Sequences flagged as poor quality0
Sequence length124
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG822150.2798174099080855No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAACGAC11820200.0117.6804351
ACTGTCT8592400.0117.594381
TAAGGGG4364550.0117.59398118
TGTCTAA8610550.0117.338873
CTGTCTA8626250.0117.219162
GTCTAAC8595950.0117.175694
CTAACGG8915850.0117.159776
ACGACGT11867750.0117.014613
TAACGGC8952550.0116.888617
ACGGCCC8898450.0116.873699
AACGACG11902250.0116.7821962
CATAATG2777900.0116.7570953
GACGTGG11499700.0116.746815
CGACGTG11500050.0116.725614
TGGTCCC11778200.0116.6355749
CATCCAA2848350.0116.590356
AGGACCA2859500.0116.559111
ACGTGGT11843200.0116.555126
TCTAACG9032450.0116.530945
ATCCAAC2870400.0116.469287
Produced by FastQC (version 0.11.5)