FastQCFastQC Report
Wed 16 Oct 2019
HVMTNAFXY_n02_GDO2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHVMTNAFXY_n02_GDO2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences111939601
Sequences flagged as poor quality0
Sequence length124
%GC51

[WARN]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGCTGTAGCTCTGAAACTCGCTGGCCGCACCGGCAGCCCCCATATAAGA3765940.3364260696266016No Hit
CTGCTGTAGCTCTGAAACGATCGTTCGGAGCCGGCTGACCCCATATAAGA3594240.321087440717249No Hit
CTGCTGTAGCTCTGAAACGCCTGTGTACGCGCGTTGCCCCCCATATAAGA2714870.24252989788662907No Hit
CTGCTGTAGCTCTGAAACCTTGGAGCTCGTGTGGGGTCCCCCATATAAGA2552960.22806584776016844No Hit
CTGCTGTAGCTCTGAAACACTTACCCACAGGGGCTCCCCCCATATAAGAA2484290.22193128953532718No Hit
CTGCTGTAGCTCTGAAACCTGGTTCACTCCTCCTGAACCCCCATATAAGA2438070.21780227714050904No Hit
CTGCTGTAGCTCTGAAACTGCAGAGTGAGCGGCACCGCCCCCATATAAGA2365850.21135058360624315No Hit
CTGCTGTAGCTCTGAAACTCACCGCGAAGCCCAGGAGCCCCCATATAAGA2262880.2021518729551305No Hit
CTGCTGTAGCTCTGAAACCCATGGGGACTGTGGGCAGCCCCCATATAAGA2067650.1847112176145777No Hit
CTGCTGTAGCTCTGAAACCCGCGAAAGTTGCTGCTGCCCCCCATATAAGA2042250.18244213680911728No Hit
CTGCTGTAGCTCTGAAACAAGCAGAATTGCAGCTGCCGCCCCATATAAGA1930530.1724617546206905No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG1872010.1672339353791336No Hit
CTGCTGTAGCTCTGAAACCATAACTTCACTACTACGTGCCCCATATAAGA1868100.16688463986931668No Hit
CTGCTGTAGCTCTGAAACTCACCCCCTCAGACACGGGCCCCCATATAAGA1861250.16627270272296218No Hit
CTGCTGTAGCTCTGAAACGAGCTTCTCCTCCTTGGGCCCCCCATATAAGA1672080.14937341075568064No Hit
CTGCTGTAGCTCTGAAACCGTGGTGCCGGCCCCTGTGCCCCCATATAAGA1543200.1378600590152184No Hit
CTGCTGTAGCTCTGAAACTGGCACTTCCAGGCTGCGCCCCCCATATAAGA1528030.13650486390424066No Hit
CTGCTGTAGCTCTGAAACGCGTGAGTCACCTCTTGCCTCCCCATATAAGA1483770.13255094593378083No Hit
CTGCTGTAGCTCTGAAACGAACAAGCACTGTAAGGGGACCCCATATAAGA1478370.13206854292789555No Hit
CTGCTGTAGCTCTGAAACTGTATGTTGTCACGCAGCACCCCCATATAAGA1455630.1300370902697786No Hit
CTGCTGTAGCTCTGAAACTTGACATTCGATCGCTACTGCCCCATATAAGA1418980.12676300320205716No Hit
CTGCTGTAGCTCTGAAACCTCTGGTAGTGGAAGAAGCCCCCCATATAAGA1390320.12420269391526598No Hit
CTGCTGTAGCTCTGAAACCGGTAGGCTTGGCCCGCGGCCCCCATATAAGA1338180.11954482489177357No Hit
CTGCTGTAGCTCTGAAACTGTCACATGATGGTCCGTCCCCCCATATAAGA1324820.11835132412165736No Hit
CTGCTGTAGCTCTGAAACTCCTGAGCTGCGCCGTGCCACCCCATATAAGA1317960.11773849363640308No Hit
CTGCTGTAGCTCTGAAACGTTCAGATGTTGCTGTTTCCCCCCATATAAGA1287850.1150486502091427No Hit
CTGCTGTAGCTCTGAAACCGCCATGTGGGGGCTCCTGCCCCCATATAAGA1284440.11474402164431514No Hit
CTGCTGTAGCTCTGAAACCTCTCAGGCCCGGGCTGCTGCCCCCATATAAG1280310.11437507267870285No Hit
CTGCTGTAGCTCTGAAACTCTTTTGCTGCTGGTGCTCCCCCCATATAAGA1261450.1126902355137035No Hit
CTGCTGTAGCTCTGAAACTTCTGAAGCCGGTGCTGCTGCCCCATATAAGA1259130.11248298088895278No Hit
CTGCTGTAGCTCTGAAACATTTTCCAAGGCTCCGGGCCCCCCATATAAGA1254680.11208544507854731No Hit
CTGCTGTAGCTCTGAAACCTGTCTTCGTCGAGGCAACCCCCCATATAAGA1249860.1116548557288497No Hit
CTGCTGTAGCTCTGAAACTTGTTACTGTAGAGCTATTCCCCCATATAAGA1222230.10918656034873664No Hit
CTGCTGTAGCTCTGTTTAGAAGATGACAGGGCTCTCGAAGTCTCACCTGT1214410.10848796932910276No Hit
CTGCTGTAGCTCTGAAACCTGAGCCGCCTCGGTCCGGCCCCCATATAAGA1169520.10447777100795633No Hit
CTGCTGTAGCTCTGAAACCCGCGCGCGTGCGGCCGGAGCCCCATATAAGA1164550.10403378157476191No Hit
CTGCTGTAGCTCTGAAACTTGGGTGAGGCGAGCGCGGGCCCCATATAAGA1161450.10375684651582776No Hit
CTGCTGTAGCTCTGAAACCAGTAGCAGAGGCCATGTTCCCCCATATAAGA1137990.10166107345692611No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTGTAGC108958650.0117.6320654
CTGCTGT108965400.0117.5229641
GTAGCTC109387100.0117.301646
TGTAGCT109152250.0117.292855
TAGCTCT109247150.0117.284227
TGCTGTA109373000.0117.233712
GCTCTGA108314800.0117.2108469
GCTGTAG109331750.0117.141193
AGCTCTG109288300.0116.967968
CGGTGGG17376700.0112.482704118
GTAGCAC486700.092.979546
CGGTGGT78161000.092.80079118
TAGCACT490550.089.528797
TGCAGTA639300.081.7744752
CGATGGT597400.078.72225118
ATGTAGC477150.070.48774
TAACTCT314250.067.052887
GTAGCTA291200.067.035096
TGTAACT310350.066.744855
GCAGTAG765900.065.576023
Produced by FastQC (version 0.11.5)