FastQCFastQC Report
Wed 16 Oct 2019
HVMTNAFXY_n01_undetermined.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHVMTNAFXY_n01_undetermined.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17717107
Sequences flagged as poor quality0
Sequence length26
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTTGAG14250.018.0998158
TGACTAG15700.017.6483651
GACCTGG11250.017.605041
AGGACGA7450.017.5786028
TTGAGAC13400.017.4576810
GACTAGC12850.017.3538112
CTTGAGA13600.017.2742129
CACCGAA16500.017.267538
GGGTACT15600.017.1148246
TCTATCG16450.016.9553818
GTAACTG12700.016.933981
ACTGAGG4850.016.9066144
CCGGGAT8000.016.8798331
CTCGAGG7200.016.8103681
ATGCGAT8250.016.7320611
ACGAGGA9450.016.7243651
GGGAATG8350.016.6514741
AGCTGAG9750.016.50808510
GGTACTC15900.016.4724717
CGTAACA17300.016.299863
Produced by FastQC (version 0.11.5)