FastQCFastQC Report
Wed 16 Oct 2019
HVMTNAFXY_n01_cDNA_04.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHVMTNAFXY_n01_cDNA_04.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3227988
Sequences flagged as poor quality0
Sequence length26
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGGGAT9100.017.9212651
CGGACTG8950.017.7744641
GCGCGAT8400.017.747181
GGGACCT8700.017.7102181
GTAACTG9150.017.385951
TCTGGAA8100.017.1692851
GATGAAA9800.017.1516381
AGGATGG6650.017.1410128
CAATGGA10150.017.141018
CTCGAAA7900.017.0973631
TTGAACG9350.017.014061
GAAATGA10000.017.0087091
ACGAGGA7650.017.002171
GGAAAGC7950.016.9898321
TACGGAT9100.016.9317461
TGACGGC7450.016.9214151
GAATGAA9300.016.890371
ATGCGAT9150.016.8392221
TATTGAG6000.016.8362163
CTGCGGA7250.016.8362061
Produced by FastQC (version 0.11.5)