FastQCFastQC Report
Wed 16 Oct 2019
HVMTNAFXY_n01_cDNA_03.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHVMTNAFXY_n01_cDNA_03.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5692582
Sequences flagged as poor quality0
Sequence length26
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGAGGA13650.017.8863241
GATGAAA17750.017.7008931
GACTAAC17450.017.5464761
GGGACCT16050.017.5184291
CCGGGAT13850.017.4112991
GGGAATG17250.017.343871
GAATGAA15350.017.3395231
ATGCGAT17450.017.317111
GCGCGAT12550.017.3013461
GGATTAC19200.017.2500611
CAATGGA16250.017.1668428
ACTGATG10550.017.0719811
ACTGAAC15250.017.0595441
CTCGAAA13500.016.9732741
CTGCGGA13100.016.9568651
TACGGGC14950.016.7995031
TACGGAT15700.016.7617741
CGGACTG13850.016.6888371
ATTGGTG14600.016.6539541
CGCTGGA13000.016.6254841
Produced by FastQC (version 0.11.5)