FastQCFastQC Report
Wed 16 Oct 2019
HVMTNAFXY_n01_cDNA_02.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHVMTNAFXY_n01_cDNA_02.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4550326
Sequences flagged as poor quality0
Sequence length26
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGGACCT12350.018.3115181
ACGAGGA11150.017.7694191
GGATTAC15100.017.693641
ACTGAAC10200.017.6585561
CGGACTG12250.017.5624581
GGGAATG13050.017.5593261
GCGCGAT11650.017.3503551
CCGGGAT10750.017.3135991
GATGAAA15500.017.1724741
GGAAAGC9900.017.081831
CTCGAAA10650.017.006381
GAAATGA12650.016.9280191
GAATGAA12600.016.9157771
GACCTGG11400.016.853081
ATGCGAT12400.016.7851241
CACCGAA7950.016.7268498
AAACCTG12500.016.6508431
TGACGGC11200.016.6179641
CAATGGA12650.016.5980998
TGACTAG10200.016.5794221
Produced by FastQC (version 0.11.5)