FastQCFastQC Report
Wed 16 Oct 2019
HVMTNAFXY_n01_cDNA_01.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHVMTNAFXY_n01_cDNA_01.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4520300
Sequences flagged as poor quality0
Sequence length26
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGGACCT13200.017.7380851
CAATGGA13800.017.6055988
CTGCGGA9650.017.5236361
GGAAAGC11900.017.4055671
GACCTGG11800.017.3834781
CCGGGAT11700.017.2754881
ACTGAAC10600.017.2746811
CGGACGT12950.017.2305811
CGCTGGA10050.017.224431
AAGGAAG6800.017.20285610
GGGAATG11000.017.1922991
AGGATGG8450.017.1567468
GATGAAA12900.017.1422331
GAATGAA10100.017.139161
TGCGGGT10650.017.0056721
TACGGAT13200.016.9800471
GCGCGAT11500.016.9668661
GGATTAC14700.016.9491081
ACGAGGA11400.016.9401511
ATGCGAT13300.016.8523791
Produced by FastQC (version 0.11.5)