FastQCFastQC Report
Wed 16 Oct 2019
HVMTNAFXY_n01_HTO.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHVMTNAFXY_n01_HTO.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences29381660
Sequences flagged as poor quality0
Sequence length26
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGATTAC180300.018.2869431
ATAACGC148500.018.011671
GATTACG131650.017.8635392
GAAATGA113000.017.834561
AGGACGG80950.017.76113910
TTGAACG111550.017.5909561
CAATGGA101650.017.5574048
TATGAGA52200.017.44924410
ATTACGT147050.017.3937823
TCGCGAC141000.017.37303510
GAATGAA82750.017.3647231
GAGGACG83150.017.2912399
GGGAATG65400.017.2588861
TGGACGC105150.017.2532121
CATATGA53050.017.2262338
ACGAGGA74650.017.1980111
AAATGCC105150.017.1865961
GCGCGAT75750.016.9482731
TGACGGC69900.016.834941
GATGAAA66500.016.7777841
Produced by FastQC (version 0.11.5)