FastQCFastQC Report
Wed 16 Oct 2019
HVMTNAFXY_n01_GDO2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHVMTNAFXY_n01_GDO2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences111939601
Sequences flagged as poor quality0
Sequence length26
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGACTAG575000.018.4466921
CTTGAGA549400.018.3697369
TTGAGAC551850.018.29180510
TCTTGAG594750.018.2836538
CACCGAA508350.018.2324568
ATCGAAG557200.018.1934722
TGGACGC559050.018.156791
ACCGAAA412850.017.9333349
GGACGCT445400.017.902282
GACTAGC511150.017.8735522
TGAGCCG523700.017.7276951
GAGAACG390850.017.645810
AGGACGA245950.017.6388848
GGACGAA245950.017.6226229
GGGTACT613300.017.6201576
TTGGAAC384000.017.5946921
TGGTAAC602150.017.5940389
GTAACTG440050.017.5638921
CCGTGGA465950.017.5131861
CCGGGAT306450.017.4953881
Produced by FastQC (version 0.11.5)