FastQCFastQC Report
Wed 16 Oct 2019
HVMNHAFXY_3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHVMNHAFXY_3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences166664679
Sequences flagged as poor quality0
Sequence length91
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACATGGGGAGACCTCAGGTCAGGCGGG11655430.6993341402589567No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG3631620.21789979867299897No Hit
GCAGTGGTATCAACGCAGAGTACATGGGGAGACCTCAGGTCAGGCGGGGC2883650.17302106344920268No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAGACCTCAGGTCAGGCGGGG2460020.1476029603129047No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGCGACCCCAGGTCAGGCGGG2054360.12326307003537325No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGCAGT45793600.083.0447161
AGCAGTG49887900.076.126182
GCAGTGG57734150.065.710343
CAGTGGT61376250.061.756084
AGTGGTA63956550.059.3371435
GTGGTAT67411350.056.236696
TGGTATC67490600.056.0738267
GGTATCA68184700.055.5147258
GTATCAA68597250.055.2165349
CAACGCA68402450.030.4533912-13
ATCAACG68523350.030.42926610-11
ACGCAGA68815100.030.30282414-15
AGAGTAC68821700.030.15998818-19
GCAGAGT69014250.030.13956616-17
AGTACAT68680400.030.09656120-21
TACATGG68567850.030.05302422-23
CATGGGG28938100.029.5527424-25
CATGGGT15076800.029.14101424-25
CATGGGC6116550.028.72285324-25
CATGGGA20362950.028.3647824-25
Produced by FastQC (version 0.11.5)