FastQCFastQC Report
Wed 16 Oct 2019
HVMNHAFXY_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHVMNHAFXY_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences166664679
Sequences flagged as poor quality0
Sequence length8
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ACTGTGGC3996833523.98128700082877No Hit
CTAAGTTT3780737222.684693737657515No Hit
GGGTCAAG3739842522.43932261136146No Hit
TACCACCA3723593522.341827448634152No Hit
GGGGGGGG72915324.374971375908629No Hit
CTAAGTTA4370390.2622265273135647No Hit
TACCCCCA2571800.1543098402991554No Hit
ACTGTGCA2101300.12607950362416023No Hit
NNNNNNNN1896750.11380635725461662No Hit
TACCACAA1874010.11244194098258815No Hit
GGGTAAAG1766940.10601766436666524No Hit
GGTCAAGA1726950.10361823575107956No Hit
CAAGTTTA1667460.10004879318190749No Hit

[WARN]Adapter Content

Can't analyse adapters as read length is too short

[OK]Kmer Content

No overrepresented Kmers

Produced by FastQC (version 0.11.5)