FastQCFastQC Report
Wed 16 Oct 2019
HVMNHAFXY_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHVMNHAFXY_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences166664679
Sequences flagged as poor quality0
Sequence length28
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGG2005310.12032003493673667No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAACGCT614050.018.0697171
GTCTGAC492550.017.5135547
ACGTAGT898800.017.4833811
AGGCTAG584350.017.4145373
TGGGAAG504300.017.3883081
GCTAGAC471750.017.3110075
GGGGTGC238350.017.2281675
ACGCTTC559400.017.1628153
GGGAAGG417750.017.1525212
GTGCAAA483650.017.135265
GCGGAGG220500.017.1212314
TGCAAAT757200.016.9984236
CCTAAGA589800.016.978241
AGGAATA590400.016.884551
TAGTAGT850250.016.8704724
GCAAATG796450.016.793967
TGCGGAG323300.016.7808383
CGGAGGA214150.016.7556845
CGTGCAA697850.016.6313614
CAAATGG412400.016.6127688
Produced by FastQC (version 0.11.5)