FastQCFastQC Report
Mon 16 Nov 2020
HVMCCDRXX_l02_n02_undetermined.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHVMCCDRXX_l02_n02_undetermined.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences23233946
Sequences flagged as poor quality0
Sequence length150
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG6878732.960637852907121No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA412970.17774423681625154No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATGCCGT46550.014.1785574
GGGGAAT76800.07.68858341
CTGGAAT66650.06.56256531
CGGAAAT106450.06.50579641
CGGCTCT62750.06.5057421
GGAGAAT144800.06.44412471
CCGGAAT53100.06.31518131
GGGAATT71800.06.0711692
GTAGATC310450.05.9984665140-144
AGATCTC293750.05.9230595140-144
AGTGTAG362900.05.8929057140-144
TCTCGGT244100.05.8897295140-144
TTCACCT53800.05.8872377
TAGATCT306000.05.8693604140-144
GTGTAGA348750.05.8307657140-144
GGGAAAC79750.05.6673951
CGAGAAT157000.05.6647761
GAGTGTA412450.05.6629777140-144
GGGAAAT104350.05.5888191
AATTGAA241050.05.573515
Produced by FastQC (version 0.11.5)