FastQCFastQC Report
Mon 16 Nov 2020
HVMCCDRXX_l02_n02_bulk_8_run_3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHVMCCDRXX_l02_n02_bulk_8_run_3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences36195187
Sequences flagged as poor quality0
Sequence length150
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG548280.1514787035082869No Hit
CAAAAATTAAAGTTCAACTGTTTAATAAAAACATTGTTTTTAGAGTATAA411110.11358139964852233No Hit
GTTAAAAGAAAAGAAACTTTAATAAATATTTTTTCCATAGATGAATGATT370620.1023948294561926No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTTAAAA643900.011.6439381
GGGGAAT231400.011.1328831
CAAAAAT1147000.010.5441931
GGTCATA189300.010.3845724
TAATCCC184150.010.2045189
GTTGAAT734800.09.6535521
AAAGTTC697000.09.6170239
ATTAAAG854750.09.3763256
GTTTAAT812950.09.2581291
TTAAAGT821450.08.9576387
GGATAGT286300.08.8216421
TTAAAAG827300.08.7650512
AATTAAG848700.08.6541135
GTCATAA235600.08.5883075
GGAAAAT730450.08.5354411
GGGAAAT362050.08.4109811
TAAAGTT840950.08.381788
CGGGAAT212900.08.3380061
CGGAAAT395650.08.097941
CCTCGAT544900.08.0914651
Produced by FastQC (version 0.11.5)