FastQCFastQC Report
Mon 16 Nov 2020
HVMCCDRXX_l02_n02_bulk_7_run_3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHVMCCDRXX_l02_n02_bulk_7_run_3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences40901130
Sequences flagged as poor quality0
Sequence length150
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG583990.14278089627352594No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGGGAAT254950.011.8032411
GTTAAAA722400.010.9085021
GGATAGT320050.010.3719451
GGTCATA204600.09.9951654
CAAAAAT1253600.09.8782161
GTTTAAT863800.09.7911711
GTTGAAT798550.09.60619451
TAATCCC214800.08.9830579
ATTAAAG921000.08.8113316
AAAGTTC753450.08.7531569
CTCCATT204300.08.7409896
AATTAAG921150.08.7395435
GATAGTT394600.08.4308662
GACCCTA245750.08.3204817
TTAAAGT871700.08.27607257
CGGGAAT245950.08.2741331
CCTCGAT588050.08.2588781
TTAAAAG917000.08.0882632
GGGAATT382350.08.0229832
CCATTCA200350.07.9778758
Produced by FastQC (version 0.11.5)