FastQCFastQC Report
Mon 16 Nov 2020
HVMCCDRXX_l02_n02_bulk_56_run_3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHVMCCDRXX_l02_n02_bulk_56_run_3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences18779414
Sequences flagged as poor quality0
Sequence length150
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG541680.288443505212676No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGGGAAT87450.08.3342351
ATCTCGG698450.07.974525140-144
TAGATCT804500.07.932816140-144
TCTCGGT640500.07.882182140-144
TGGTCGC399000.07.816984140-144
AGTGTAG1001600.07.6642213140-144
GGAAAAT380650.07.62086771
GTAGATC900700.07.598736140-144
AGATCTC817100.07.584916140-144
TCGGTGG621650.07.5791593140-144
GTGTAGA971100.07.5638876140-144
GGTGGTC489300.07.557419140-144
GGGAAAT189900.07.48592421
CTCGGTG644900.07.399695140-144
CAAAAAT464300.07.36688381
TGTAGAT970500.07.3578725140-144
GGTCGCC399650.07.310649140-144
GAGTGTA1128650.07.2811885140-144
GGATAGT105300.07.2640981
CCGTATC199650.07.226901140-144
Produced by FastQC (version 0.11.5)