FastQCFastQC Report
Mon 16 Nov 2020
HVMCCDRXX_l02_n02_bulk_55_run_3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHVMCCDRXX_l02_n02_bulk_55_run_3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences19502122
Sequences flagged as poor quality0
Sequence length150
%GC46

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG218320.11194679225163293No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CAAAAAT492000.09.475341
GGATAGT114050.08.5421241
TGGTCGC400700.08.153973140-144
ATCTCGG702750.08.026141140-144
TAGATCT809600.08.014469140-144
TCTCGGT650750.07.992593140-144
GTGTAGA988300.07.90433140-144
GTTTAAT311950.07.8654091
AGTGTAG1017900.07.8414054140-144
ATTAAAG338950.07.83887626
GGTGGTC501700.07.8126454140-144
TCGGTGG624250.07.785192140-144
GTAGATC911850.07.763232140-144
CTCGGTG655900.07.727858140-144
GGTCGCC382400.07.7119846140-144
AGATCTC822150.07.636415140-144
GGGAAAT199800.07.62104231
TTAAAGT290550.07.58271367
GAGTGTA1146400.07.503833140-144
GGAAAAT396350.07.4832521
Produced by FastQC (version 0.11.5)