FastQCFastQC Report
Mon 16 Nov 2020
HVMCCDRXX_l02_n02_bulk_54_run_3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHVMCCDRXX_l02_n02_bulk_54_run_3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences19760680
Sequences flagged as poor quality0
Sequence length150
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG207680.10509759785594422No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGGGAAT92300.09.303751
CAAAAAT510750.08.4772631
TCTCGGT705650.08.168645140-144
GGATAGT113300.08.1525381
ATCTCGG762200.08.102907140-144
TCGGTGG662150.07.9963355140-144
GGTGGTC530150.07.8768578140-144
TGGTCGC436500.07.8348947140-144
TAGATCT864600.07.8277392140-144
CTCGGTG707400.07.796281140-144
GGGAAAT204500.07.76322461
GCCGTAT234200.07.7224755140-144
GGTCGCC429850.07.5909605140-144
AGATCTC875200.07.497656140-144
CGCCGTA283850.07.4877477140-144
CCGTATC221100.07.4310584140-144
GGAGAAT320700.07.42553571
AGTGTAG1083500.07.4171467140-144
GTGTAGA1050950.07.4153132140-144
GTAGATC973200.07.402564140-144
Produced by FastQC (version 0.11.5)