FastQCFastQC Report
Mon 16 Nov 2020
HVMCCDRXX_l02_n02_bulk_53_run_3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHVMCCDRXX_l02_n02_bulk_53_run_3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16805637
Sequences flagged as poor quality0
Sequence length150
%GC46

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG196330.11682389664848765No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGGAAAT172850.08.2663131
GGGGAAT83900.08.1710461
GGAAAAT344300.08.0274531
ATCTCGG637750.07.9974575140-144
CAAAAAT419900.07.99140451
GGATAGT94100.07.89884571
TCTCGGT596250.07.870636140-144
TAGATCT729900.07.840041140-144
TGGTCGC371000.07.7975807140-144
GCCGTAT202550.07.770371140-144
TCGGTGG561750.07.759311140-144
GTGTAGA879050.07.6826863140-144
GGTGGTC449100.07.675999140-144
AGTGTAG907750.07.6428337140-144
CTCGGTG595100.07.614837140-144
GTAGATC811500.07.5698304140-144
GGTCGCC353750.07.550945140-144
GTTAAAA247950.07.50881861
CGCCGTA244000.07.5008335140-144
AGATCTC736350.07.4897676140-144
Produced by FastQC (version 0.11.5)