FastQCFastQC Report
Mon 16 Nov 2020
HVMCCDRXX_l02_n02_bulk_52_run_3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHVMCCDRXX_l02_n02_bulk_52_run_3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17694364
Sequences flagged as poor quality0
Sequence length150
%GC46

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG221490.12517545134710692No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AATCCAA171750.08.6785395
TAGATCT717000.08.0774975140-144
ATCTCGG633850.07.9898705140-144
CAAAAAT435700.07.98304751
GGGGAAT85300.07.95221421
TCTCGGT591250.07.932325140-144
TGGTCGC359800.07.808183140-144
TCGGTGG559000.07.722781140-144
AGTGTAG905100.07.685894140-144
GGTGGTC443250.07.6798487140-144
CGGGAAT121650.07.65220981
GTAGATC803450.07.649261140-144
AGATCTC737500.07.6421140-144
GTGTAGA875500.07.6167994140-144
CTCGGTG591450.07.581488140-144
CCGGGAT74700.07.5351
AAATCCA209600.07.52360254
CGGAAAT206750.07.5041621
GGAGAAT283200.07.4659251
GGTCGCC358900.07.422534140-144
Produced by FastQC (version 0.11.5)