FastQCFastQC Report
Mon 16 Nov 2020
HVMCCDRXX_l02_n02_bulk_51_run_3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHVMCCDRXX_l02_n02_bulk_51_run_3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17700836
Sequences flagged as poor quality0
Sequence length150
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG254250.14363728357236913No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGGGAAT86000.09.062461
GGGAAAT184250.08.2249371
ATCTCGG644700.08.15246140-144
CGGAAAT208600.08.0259131
TCTCGGT594150.07.963885140-144
TAGATCT737300.07.8531404140-144
AGTGTAG921550.07.809637140-144
GAATTGA216750.07.74065974
GGATAGT102700.07.72935061
TGGTCGC367500.07.719024140-144
CTCGGTG591850.07.6955748140-144
GTGTAGA891950.07.681348140-144
GGTCGCC357250.07.6664047140-144
TCGGTGG569000.07.6477857140-144
GTAGATC825150.07.615622140-144
CAAAAAT438800.07.59794431
GTTTAAT265950.07.5705071
GGTGGTC448700.07.528795140-144
GGAAAAT362700.07.52081731
AAATCCA212550.07.48707774
Produced by FastQC (version 0.11.5)