FastQCFastQC Report
Mon 16 Nov 2020
HVMCCDRXX_l02_n02_bulk_50_run_3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHVMCCDRXX_l02_n02_bulk_50_run_3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15182421
Sequences flagged as poor quality0
Sequence length150
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG485620.31985676065760527No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGGAAAT161300.08.4106491
CAAAAAT383300.08.0388721
TAGATCT570350.08.0386915140-144
ATCTCGG506400.07.822592140-144
TCTCGGT463050.07.814809140-144
AGTGTAG717600.07.611218140-144
GTAGATC641050.07.610361140-144
AGATCTC588850.07.6100707140-144
GTGTAGA691800.07.576604140-144
TGGTCGC285850.07.526033140-144
CGGAAAT177900.07.4635921
GAGTGTA817550.07.401086140-144
TCGGTGG446700.07.3594217140-144
TGTAGAT695900.07.289866140-144
CTCGGTG468150.07.243686140-144
GGATAGT81300.07.18952941
GGTCGCC284200.07.1339874140-144
ATTAAAG257950.07.0625296
GGGGAAT73750.07.04492761
GGTGGTC356400.07.042282140-144
Produced by FastQC (version 0.11.5)