FastQCFastQC Report
Mon 16 Nov 2020
HVMCCDRXX_l02_n02_bulk_49_run_3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHVMCCDRXX_l02_n02_bulk_49_run_3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences20606659
Sequences flagged as poor quality0
Sequence length150
%GC46

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CAAAAAT537600.08.7660071
AGTGTAG879200.08.326669140-144
TAGATCT697200.08.267642140-144
GTGTAGA849950.08.084637140-144
TTAAAGT302600.08.0183397
ATCTCGG614800.07.9774528140-144
GTAGATC780950.07.8972936140-144
ATTAAAG365150.07.76964336
TCTCGGT559350.07.761705140-144
GAGTGTA1007700.07.7478585140-144
GGATAGT119400.07.61577461
TGTAGAT867050.07.558161140-144
AGATCTC715850.07.482976140-144
GGTGGTC429950.07.455223140-144
TCGGTGG541550.07.4318466140-144
AGAGTGT1124850.07.4158745140-144
CTCGGTG564550.07.4147425140-144
TAAAGTT307700.07.37066278
TGGTCGC354650.07.3328238140-144
CGGAAAT244250.07.32765441
Produced by FastQC (version 0.11.5)