FastQCFastQC Report
Thu 12 Nov 2020
HVMCCDRXX_l02_n02_bulk_48_run_3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHVMCCDRXX_l02_n02_bulk_48_run_3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences927017
Sequences flagged as poor quality0
Sequence length150
%GC50

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG83870.904729902472123No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGCTATT2952.2180863E-417.1076551
AACCTGG3559.1562E-414.197055
CGGTTTC6252.806948E-612.6718828
AATTGGT7701.770004E-712.155735
GGAACAT7009.790998E-611.3294571
AATGTCC4550.005898381611.0768195
CTCGAAT7251.4450861E-510.93878651
ATCGGTT6758.547818E-510.6665666
AACTTGC4750.00811992410.6104275
GTTGTAC5450.001865308110.5687085
TTGATGC4800.008775542510.4999017
ATTTACG7652.6554842E-510.3528456
GGCAAAC5750.002878575610.0308231
GTTGGAC5800.00309264739.944351
GGGGAAT5850.00332041629.8593571
TCGGATT5900.00360260289.7626217
CTTTGAT6100.00469157579.4552851
GGATAGT7652.982017E-49.4243851
GGAATTG9201.9452296E-59.3912153
GGGAAAT10005.0229846E-69.3725511
Produced by FastQC (version 0.11.5)