FastQCFastQC Report
Mon 16 Nov 2020
HVMCCDRXX_l02_n02_bulk_48_run_3_corrected.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHVMCCDRXX_l02_n02_bulk_48_run_3_corrected.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17002589
Sequences flagged as poor quality0
Sequence length150
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG439940.25874882936945665No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGGGAAT81400.08.9541
GGATAGT93400.08.8852791
TGGTCGC433950.07.900849140-144
TAGATCT872750.07.881691140-144
ATCTCGG770800.07.838768140-144
TCTCGGT708550.07.818181140-144
GCCGTAT239200.07.7837954140-144
CCGTATC229250.07.6568193140-144
TCGGTGG676500.07.6074796140-144
GGTGGTC527850.07.589281140-144
GGTCGCC426000.07.575064140-144
GTAGATC983750.07.5515275140-144
AATCCAA160500.07.5370115
AGATCTC883200.07.5340934140-144
GTGTAGA1065450.07.529292140-144
CTCGGTG703000.07.5255437140-144
AGTGTAG1104900.07.4859257140-144
CAAAAAT385250.07.38029341
CCATTCA78050.07.3797418
TGTAGAT1054600.07.324114140-144
Produced by FastQC (version 0.11.5)