FastQCFastQC Report
Thu 12 Nov 2020
HVMCCDRXX_l02_n02_bulk_47_run_3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHVMCCDRXX_l02_n02_bulk_47_run_3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences21771626
Sequences flagged as poor quality0
Sequence length150
%GC46

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG485970.2232125427838968No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGATAGT136250.013.6536221
TAGTTAA170550.010.4274734
GATAGTT174750.010.4240672
CAAAAAT533900.09.426711
GTTTAAT350850.09.412581
GTTAAAA335400.09.1582231
TTAAAGT322950.08.9845777
ATAGTTA208650.08.4543753
ATTAAAG375950.08.3500736
TAAAGTT330300.08.2396958
TGGTCGC603700.08.186231140-144
ATCTCGG1031550.08.029462140-144
TTAAAAG358800.08.0267812
GGTCGCC579100.08.016768140-144
AAAGTTC332050.08.001129
GGTGGTC738850.07.9575796140-144
TCTCGGT961500.07.9509916140-144
TCGGTGG905150.07.827128140-144
GGAAAAT440350.07.8269861
GCCGTAT312550.07.7954054140-144
Produced by FastQC (version 0.11.5)