FastQCFastQC Report
Mon 16 Nov 2020
HVMCCDRXX_l02_n02_bulk_47_run_3_corrected.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHVMCCDRXX_l02_n02_bulk_47_run_3_corrected.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences21986451
Sequences flagged as poor quality0
Sequence length150
%GC46

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG495870.2255343529521886No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGATAGT135000.014.7006681
GATAGTT176300.011.1097542
TAGTTAA178050.010.9597424
GTTAAAA326950.09.491291
ATAGTTA213250.08.9143133
CTCCATT114200.08.7013386
CAAAAAT535700.08.5274711
CCATTCA106150.08.47839458
TGGTCGC602050.08.208586140-144
TTAAAAG366500.08.0752452
GGTGGTC738100.08.028017140-144
ATCTCGG1030100.08.012729140-144
TCTCGGT955400.08.000172140-144
GGGGAAT104800.07.98794131
TCGGTGG903850.07.960981140-144
GTTTAAT351900.07.8954991
GGTCGCC582550.07.823375140-144
TCCATTC135500.07.81091077
TAGATCT1178050.07.759376140-144
CTCGGTG950350.07.7578254140-144
Produced by FastQC (version 0.11.5)