FastQCFastQC Report
Mon 16 Nov 2020
HVMCCDRXX_l02_n02_bulk_35_run_3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHVMCCDRXX_l02_n02_bulk_35_run_3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences38705718
Sequences flagged as poor quality0
Sequence length150
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG859330.22201629226978817No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCCATT197650.08.8529396
GGGGAAT187500.08.5058271
GGATAGT232800.08.4316291
GGAAAAT801500.07.9142711
CAAAAAT1052150.07.83960441
CCATTCA208500.07.8386878
GGGAAAT386700.07.78193241
TCCATTC242550.07.74752147
AGTGTAG760850.07.6403494140-144
CGGGAAT250700.07.5705421
CGGCTCT239650.07.37758541
GTAGGGA1228000.07.2221313140-144
GAATTGA487700.07.2199484
TGTAGGG1250350.07.216263140-144
CGGAAAT453050.07.19975761
GTGTAGA769350.07.1928325140-144
GAGTGTA916700.07.0435557140-144
GAATCCA325300.07.03916264
GTGTAGG1381150.06.9019322140-144
GTAGATC727050.06.880485140-144
Produced by FastQC (version 0.11.5)