FastQCFastQC Report
Mon 16 Nov 2020
HVMCCDRXX_l02_n02_bulk_34_run_3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHVMCCDRXX_l02_n02_bulk_34_run_3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences41036590
Sequences flagged as poor quality0
Sequence length150
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG642930.15667237458083141No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGGGAAT233650.09.0188981
GGAAAAT838800.08.0615321
CGGGAAT275950.07.97638131
AGTGTAG841500.07.888607140-144
CAAAAAT1070750.07.68339541
CGGAAAT485450.07.50729131
GTGTAGA846900.07.454043140-144
TGTAGGG1421550.07.305452140-144
GTAGGGA1395000.07.2886233140-144
GGATAGT252400.07.20522641
GAGTGTA1024900.07.1879086140-144
GGGAAAT414650.07.1009251
TAGGGAA1318200.07.048011140-144
GTGTAGG1568300.07.034121140-144
GTAGATC803350.07.0300155140-144
CCGGAAT249650.06.9666161
GGGAATT347050.06.94529771
TAGATCT724200.06.937387140-144
CTCCATT223600.06.92380246
AATCGAA654400.06.91025975
Produced by FastQC (version 0.11.5)