FastQCFastQC Report
Mon 16 Nov 2020
HVMCCDRXX_l02_n02_bulk_25_run_3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHVMCCDRXX_l02_n02_bulk_25_run_3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences34401946
Sequences flagged as poor quality0
Sequence length150
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG551710.1603717417613527No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCATTCA167950.08.8739118
GTTAAAA537800.08.81434251
GGGGAAT174600.08.34742451
CTCCATT190350.08.2464586
TCCATTC211400.08.2420477
AGTGTAG708700.08.194463140-144
GGATAGT226900.08.1086971
CAAAAAT919950.07.88219551
TTAAAAG578150.07.7592242
GTGTAGA711600.07.728023140-144
GGGAAAT352850.07.64762971
TGTAGGG1221500.07.517573140-144
GGAAAAT693050.07.50612931
GTAGGGA1187200.07.488545140-144
GAGTGTA850500.07.461453140-144
CGGGAAT231850.07.3442981
GTAGATC668050.07.339439140-144
TAGATCT602600.07.2906756140-144
CGGAAAT397200.07.28417731
TAGGGAA1125500.07.1621284140-144
Produced by FastQC (version 0.11.5)