FastQCFastQC Report
Mon 16 Nov 2020
HVMCCDRXX_l02_n02_bulk_12_run_3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHVMCCDRXX_l02_n02_bulk_12_run_3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences38409874
Sequences flagged as poor quality0
Sequence length150
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG600950.15645716515498073No Hit
GTTAAAAGAAAAGAAACTTTAATAAATATTTTTTCCATAGATGAATGATT385740.1004273015839625No Hit
CAAAAATTAAAGTTCAACTGTTTAATAAAAACATTGTTTTTAGAGTATAA385160.10027629874547361No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAATCCC169950.012.2009829
GTTAAAA698850.011.5965851
GGGGAAT240200.011.5670361
GGTCATA208300.010.9238884
CAAAAAT1145800.010.4300371
AAAGTTC692600.09.9795789
GGATAGT303100.09.8808991
ATTAAAG857400.09.6161546
GTTTAAT804150.09.1985861
GGGAATT364150.09.17561151
AGGTCAT234100.09.0432663
TTAAAGT809100.09.032077
GTTGAAT803650.08.8810371
GTCATAA261650.08.6690055
TTAAAAG890550.08.6438192
CTCCATT204250.08.5668986
CGGCTCT203200.08.3815171
TAAAGTT836750.08.3722198
TCCATTC231650.08.3607157
GGAAAAT782250.08.3490551
Produced by FastQC (version 0.11.5)