FastQCFastQC Report
Mon 16 Nov 2020
HVMCCDRXX_l02_n02_bulk_11_run_3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHVMCCDRXX_l02_n02_bulk_11_run_3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences37014841
Sequences flagged as poor quality0
Sequence length150
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG1698910.45898076395897525No Hit
GTTAAAAGAAAAGAAACTTTAATAAATATTTTTTCCATAGATGAATGATT561800.1517769588690115No Hit
GTTTAATAAAAACATTGTTTTTAGAGTATAATTTAAAATTAGTTCTGCTC436070.11780950241012789No Hit
CAAAAATTAAAGTTCAACTGTTTAATAAAAACATTGTTTTTAGAGTATAA425080.1148404230616579No Hit
GCATAATTTAATTTTGTAGTATATATATAATTGGGATTATGACCTCGATG408740.11042597751534311No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTTAAAA732700.014.7538651
TAATCCC166450.014.663569
GGATAGT338900.013.5214211
GGTCATA198200.013.2244124
GGGGAAT256850.012.7835661
GATAGTT401500.011.1377692
TTAAAAG969900.010.6466832
GTTTAAT892250.010.5384811
AGGTCAT222300.010.5274353
AAAGTTC735500.010.3666119
TAGTTAA450050.010.1599514
GTCATAA247350.010.1308525
GGGAATT379550.09.9818371
CAAAAAT1182050.09.9694351
ATTAAAG915950.09.9212466
GAGGTCA249000.09.8615952
GTTGAAT871250.09.127651
TTAAAGT918850.09.042427
CCTCGAT658350.08.8677181
CGGCTCT191250.08.7540041
Produced by FastQC (version 0.11.5)