FastQCFastQC Report
Mon 16 Nov 2020
HVMCCDRXX_l02_n01_undetermined.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHVMCCDRXX_l02_n01_undetermined.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences23233946
Sequences flagged as poor quality0
Sequence length150
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGGTCA69550.014.5873122
CTAGACT86450.012.5679731
GTCCCAT77350.011.6051499
CGTCCCA83650.010.9028198
TAGACTT105250.010.5281462
AGACTTC107300.09.771343
CGAGGTC107450.09.1072191
ATTCTAG94000.09.0147818
TTCGTAC109250.08.6109463
CCGGGAT45250.08.5867431
CGTACAA127350.08.45855
GGCAAAT65050.08.1853591
GGCGGAT65350.08.1477821
GGCAATT59250.08.1365391
TCTAGAC82800.07.8924547
CCCCATT29950.07.6878771
TCGTACA134000.07.663594
TAATCCC181850.07.50311049
TTCTAGA117950.07.3669629
CATTCTA122150.07.2900297
Produced by FastQC (version 0.11.5)