FastQCFastQC Report
Mon 16 Nov 2020
HVMCCDRXX_l02_n01_bulk_8_run_3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHVMCCDRXX_l02_n01_bulk_8_run_3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences36195187
Sequences flagged as poor quality0
Sequence length150
%GC43

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGAGGTCATAATCCCAATTATATATATACTACAAAATTAAATTATGCTGT637140.17602892892914188No Hit
CTCCTTCATTCTAGACTTCATTTAAAAGACTATTGATTATGCTACCTTTG522250.14428713961334141No Hit
CAAATTTATAAAATTTTTTTATAGATAGAAACCGATCTGGCTTACACCGA486760.13448196855565356No Hit
CTTCATTCTAGACTTCATTTAAAAGACTATTGATTATGCTACCTTTGTAC482570.13332435608082366No Hit
CTAGACTTCATTTAAAAGACTATTGATTATGCTACCTTTGTACAGTCAAT465010.1284728823199615No Hit
CTTTCGTACAAATTTATAAAATTTTTTTATAGATAGAAACCGATCTGGCT463370.12801978340379896No Hit
GTCTTTTCGTCCCATAAATTTATTTAAGTTTTCTTACTTAAAAAATAAAT459570.12696992006147115No Hit
TTTTATTCTTAAAATAAAAAATATTTTTTATAAACAATCCTTTCGTACAA368490.10180635342483518No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTCATA443700.024.7250044
TAATCCC499750.022.2400269
GAGGTCA464450.022.0566482
AGGTCAT476350.021.4889053
GTCATAA618600.018.1882065
CGAGGTC577800.017.3678531
ATAATCC745350.015.3559878
CATAATC758350.014.92188557
TCTAGAC569950.014.0698447
GTCCCAT492000.014.0311859
CGTCCCA514950.013.4617678
TCATAAT900750.012.7946356
TCGTCCC572300.012.5781597
CTAGACT641250.012.3594958
ATTCTAG682900.012.2697788
CATTCTA714450.012.1511194
CGTACAA728700.011.2516395
TTCTAGA753550.011.1385159
TAGACTT742750.010.9031189
TTAAACC268850.010.8974587
Produced by FastQC (version 0.11.5)